pCMV-FANCA-3×FLAG-Neo plasmid

pCMV-FANCA-3×FLAG-Neo plasmid

PVT95562

pCMV-FANCA-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-FANCA(human)-3×FLAG-Neo
Alias: NM_000135.4;FA; FA-H; FA1; FAA; FACA; FAH; FANCH
Gene lenght: 4368bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FANCA-3×FLAG-Neo plasmid Description

pCMV-FANCA-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FANCA-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgtc cgactcgtgg gtcccgaact 781 ccgcctcggg ccaggaccca gggggccgcc ggagggcctg ggccgagctg ctggcgggaa 841 gggtcaagag ggaaaaatat aatcctgaaa gggcacagaa attaaaggaa tcagctgtgc 901 gcctcctgcg aagccatcag gacctgaatg cccttttgct tgaggtagaa ggtccactgt 961 gtaaaaaatt gtctctcagc aaagtgattg actgtgacag ttctgaggcc tatgctaatc 1021 attctagttc atttataggc tctgctttgc aggatcaagc ctcaaggctg ggggttcccg 1081 tgggtattct ctcagccggg atggttgcct ctagcgtggg acagatctgc acggctccag 1141 cggagaccag tcaccctgtg ctgctgactg tggagcagag aaagaagctg tcttccctgt 1201 tagagtttgc tcagtattta ttggcacaca gtatgttctc ccgtctttcc ttctgtcaag 1261 aattatggaa aatacagagt tctttgttgc ttgaagcggt gtggcatctt cacgtacaag 1321 gcattgtgag cctgcaagag ctgctggaaa gccatcccga catgcatgct gtgggatcgt 1381 ggctcttcag gaatctgtgc tgcctttgtg aacagatgga agcatcctgc cagcatgctg 1441 acgtcgccag ggccatgctt tctgattttg ttcaaatgtt tgttttgagg ggatttcaga 1501 aaaactcaga tctgagaaga actgtggagc ctgaaaaaat gccgcaggtc acggttgatg 1561 tactgcagag aatgctgatt tttgcacttg acgctttggc tgctggagta caggaggagt 1621 cctccactca caagatcgtg aggtgctggt tcggagtgtt cagtggacac acgcttggca 1681 gtgtaatttc cacagatcct ctgaagaggt tcttcagtca taccctgact cagatactca 1741 ctcacagccc tgtgctgaaa gcatctgatg ctgttcagat gcagagagag tggagctttg 1801 cgcggacaca ccctctgctc acctcactgt accgcaggct ctttgtgatg ctgagtgcag 1861 aggagttggt tggccatttg caagaagttc tggaaacgca ggaggttcac tggcagagag 1921 tgctctcctt tgtgtctgcc ctggttgtct gctttccaga agcgcagcag ctgcttgaag 1981 actgggtggc gcgtttgatg gcccaggcat tcgagagctg ccagctggac agcatggtca 2041 ctgcgttcct ggttgtgcgc caggcagcac tggagggccc ctctgcgttc ctgtcatatg 2101 cagactggtt caaggcctcc tttgggagca cacgaggcta ccatggctgc agcaagaagg 2161 ccctggtctt cctgtttacg ttcttgtcag aactcgtgcc ttttgagtct ccccggtacc 2221 tgcaggtgca cattctccac ccacccctgg ttcccggcaa gtaccgctcc ctcctcacag 2281 actacatctc attggccaag acacggctgg ccgacctcaa ggtttctata gaaaacatgg 2341 gactctacga ggatttgtca tcagctgggg acattactga gccccacagc caagctcttc 2401 aggatgttga aaaggccatc atggtgtttg agcatacggg gaacatccca gtcaccgtca 2461 tggaggccag catattcagg aggccttact acgtgtccca cttcctcccc gccctgctca 2521 cacctcgagt gctccccaaa gtccctgact cccgtgtggc gtttatagag tctctgaaga 2581 gagcagataa aatcccccca tctctgtact ccacctactg ccaggcctgc tctgctgctg 2641 aagagaagcc agaagatgca gccctgggag tgagggcaga acccaactct gctgaggagc 2701 ccctgggaca gctcacagct gcactgggag agctgagagc ctccatgaca gaccccagcc 2761 agcgtgatgt tatatcggca caggtggcag tgatttctga aagactgagg gctgtcctgg 2821 gccacaatga ggatgacagc agcgttgaga tatcaaagat tcagctcagc atcaacacgc 2881 cgagactgga gccacgggaa cacatggctg tggacctcct gctgacgtct ttctgtcaga 2941 acctgatggc tgcctccagt gtcgctcccc cggagaggca gggtccctgg gctgccctct 3001 tcgtgaggac catgtgtgga cgtgtgctcc ctgcagtgct cacccggctc tgccagctgc 3061 tccgtcacca gggcccgagc ctgagtgccc cacatgtgct ggggttggct gccctggccg 3121 tgcacctggg tgagtccagg tctgcgctcc cagaggtgga tgtgggtcct cctgcacctg 3181 gtgctggcct tcctgtccct gcgctctttg acagcctcct gacctgtagg acgagggatt 3241 ccttgttctt ctgcctgaaa ttttgtacag cagcaatttc ttactctctc tgcaagtttt 3301 cttcccagtc acgagatact ttgtgcagct gcttatctcc aggccttatt aaaaagtttc 3361 agttcctcat gttcagattg ttctcagagg cccgacagcc tctttctgag gaggacgtag 3421 ccagcctttc ctggagaccc ttgcaccttc cttctgcaga ctggcagaga gctgccctct 3481 ctctctggac acacagaacc ttccgagagg tgttgaaaga ggaagatgtt cacttaactt 3541 accaagactg gttacacctg gagctggaaa ttcaacctga agctgatgct ctttcagata 3601 ctgaacggca ggacttccac cagtgggcga tccatgagca ctttctccct gagtcctcgg 3661 cttcaggggg ctgtgacgga gacctgcagg ctgcgtgtac cattcttgtc aacgcactga 3721 tggatttcca ccaaagctca aggagttatg accactcaga aaattctgat ttggtctttg 3781 gtggccgcac aggaaatgag gatattattt ccagattgca ggagatggta gctgacctgg 3841 agctgcagca agacctcata gtgcctctcg gccacacccc ttcccaggag cacttcctct 3901 ttgagatttt ccgcagacgg ctccaggctc tgacaagcgg gtggagcgtg gctgccagcc 3961 ttcagagaca gagggagctg ctaatgtaca aacggatcct cctccgcctg ccttcgtctg 4021 tcctctgcgg cagcagcttc caggcagaac agcccatcac tgccagatgc gagcagttct 4081 tccacttggt caactctgag atgagaaact tctgctccca cggaggtgcc ctgacacagg 4141 acatcactgc ccacttcttc aggggcctcc tgaacgcctg tctgcggagc agagacccct 4201 ccctgatggt cgacttcata ctggccaagt gccagacgaa atgcccctta attttgacct 4261 ctgctctggt gtggtggccg agcctggagc ctgtgctgct ctgccggtgg aggagacact 4321 gccagagccc gctgccccgg gaactgcaga agctacaaga aggccggcag tttgccagcg 4381 atttcctctc ccctgaggct gcctccccag cacccaaccc ggactggctc tcagctgctg 4441 cactgcactt tgcgattcaa caagtcaggg aagaaaacat caggaagcag ctaaagaagc 4501 tggactgcga gagagaggag ctattggttt tccttttctt cttctccttg atgggcctgc 4561 tgtcgtcaca tctgacctca aatagcacca cagacctgcc aaaggctttc cacgtttgtg 4621 cagcaatcct cgagtgttta gagaagagga agatatcctg gctggcactc tttcagttga 4681 cagagagtga cctcaggctg gggcggctcc tcctccgtgt ggccccggat cagcacacca 4741 ggctgctgcc tttcgctttt tacagtcttc tctcctactt ccatgaagac gcggccatca 4801 gggaagaggc cttcctgcat gttgctgtgg acatgtactt gaagctggtc cagctcttcg 4861 tggctgggga tacaagcaca gtttcacctc cagctggcag gagcctggag ctcaagggtc 4921 agggcaaccc cgtggaactg ataacaaaag ctcgtctttt tctgctgcag ttaatacctc 4981 ggtgcccgaa aaagagcttc tcacacgtgg cagagctgct ggctgatcgt ggggactgcg 5041 acccagaggt gagcgccgcc ctccagagca gacagcaggc tgcccctgac gctgacctgt 5101 cccaggagcc tcatctcttc GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC 5161 ATGACATCGA CTACAAGGAT GACGATGACA AGTAGTGAGG GCCCGATATC TCGACAATCA 5221 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 5281 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 5341 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 5401 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 5461 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 5521 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 5581 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG 5641 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 5701 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT 5761 TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG 5821 CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA 5881 AGACAGAATA AAACGCACGG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA 5941 GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC 6001 TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT 6061 CGGGGCGGCA GGCCCTGCCA TAGCAGATCT GCGCAGCTGG GGCTCTAGGG GGTATCCCCA 6121 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC 6181 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC 6241 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG 6301 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC 6361 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG 6421 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 6481 AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 6541 CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 6601 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 6661 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 6721 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 6781 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG 6841 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 6901 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 6961 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 7021 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 7081 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 7141 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 7201 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 7261 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 7321 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 7381 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 7441 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 7501 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 7561 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 7621 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 7681 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 7741 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA CGCCCAACCT 7801 GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT 7861 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC 7921 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 7981 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 8041 TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC 8101 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 8161 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 8221 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 8281 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 8341 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 8401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 8461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 8521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 8581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 8641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC 8701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 8761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 8821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 8881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 8941 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 9001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 9061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 9121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 9181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 9241 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 9301 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 9361 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 9421 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 9481 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 9541 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 9601 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 9661 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 9721 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 9781 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 9841 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 9901 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 9961 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 10021 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 10081 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 10141 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 10201 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG 10261 ATCGGGAGAT CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA 10321 TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC 10381 AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.