pLV3-CMV-Ltbp3-3×FLAG-CopGFP-P

pLV3-CMV-Ltbp3-3×FLAG-CopGFP-Puro plasmid

PVT95559

pLV3-CMV-Ltbp3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ltbp3(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_008520.3;Ltbp2; mFLJ00070
Gene lenght: 3759bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ltbp3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Ltbp3-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Ltbp3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccATGC CCGGGCCCCG AGGGGCTGCC 2341 CACGGCCTGG CCCCTGCGAT GCGCCAGGCC GGGGCATTGG GGCTGCTGGC ACTACTCCTG 2401 CTGGCGCTGC TGGGCCCCGG CGGCGGGGCC GAGGGTGGGC CGGCCGGCGA GCGGGGCACA 2461 GGCGGGGGCG GGGCGCTGGC CCGCGAACGC TTCAAGGTGG TCTTTGCGCC AGTGATCTGC 2521 AAGCGGACCT GTCTGAAGGG CCAGTGTCGG GACAGCTGTC AGCAGGGCTC CAACATGACG 2581 CTCATCGGAG AGAACGGCCA CAGCACCGAC ACGCTCACCG GTTCTGGCTT CCGCGTGGTG 2641 GTGTGCCCTC TACCCTGCAT GAACGGTGGC CAGTGCTCTT CCCGAAACCA GTGCCTGTGT 2701 CCCCCGGATT TCACGGGGCG CTTCTGCCAG GTGCCTGCTG CAGGAACCGG AGCTGGCACC 2761 GGGAGTTCAG GCCCCGGCCT GGCCCGGACC GGGGCCATGT CCACAGGCCC GCTGCCGCCC 2821 CTTGCCCCAG AAGGAGAGTC TGTGGCTAGC AAACACGCCA TTTACGCGGT GCAGGTGATC 2881 GCAGATCCTC CCGGGCCGGG GGAGGGTCCT CCTGCACAAC ATGCAGCCTT CTTGGTGCCC 2941 CTGGGGCCAG GACAAATCTC GGCAGAAGTG CAGGCTCCGC CCCCCGTGGT GAACGTGCGT 3001 GTCCATCACC CTCCTGAAGC TTCCGTTCAG GTGCACCGCA TCGAGGGGCC GAACGCTGAA 3061 GGCCCAGCCT CTTCCCAGCA CTTGCTGCCG CATCCCAAGC CCCAGCACCC GAGGCCACCC 3121 ACCCAAAAGC CACTGGGCCG CTGCTTCCAG GACACATTGC CCAAGCAGCC TTGTGGCAGC 3181 AACCCTTTGC CTGGCCTTAC CAAGCAGGAA GATTGCTGCG GTAGCATCGG TACTGCCTGG 3241 GGACAAAGCA AGTGTCACAA GTGCCCACAG CTTCAGTATA CAGGGGTGCA GAAGCCTGGA 3301 CCTGTACGTG GGGAGGTGGG TGCTGACTGC CCCCAGGGCT ACAAGAGGCT CAACAGCACC 3361 CACTGCCAGG ATATCAACGA ATGTGCGATG CCTGGCATGT GTCGCCATGG TGACTGCCTC 3421 AACAACCCTG GCTCTTATCG CTGTGTCTGC CCGCCCGGTC ATAGCTTGGG TCCCTCGCGC 3481 ACACAGTGCA TTGCCGACAA ACCAGAGGAG AAGAGCCTGT GTTTCCGCCT TGTGAGCACC 3541 GAACACCAGT GCCAGCACCC TCTGACCACA CGCCTAACCC GCCAGCTCTG CTGCTGTAGT 3601 GTGGGTAAAG CCTGGGGTGC CCGGTGCCAG CGCTGCCCGG CAGATGGTAC AGCAGCCTTC 3661 AAGGAGATCT GCCCGGCTGG GAAAGGGTAC CATATCCTCA CCTCCCACCA GACGCTCACC 3721 ATCCAGGGGG AAAGCGACTT CTCCCTCTTC CTGCACCCCG ACGGGCCACC CAAACCCCAG 3781 CAGCTTCCTG AAAGCCCCAG CCGAGCACCA CCCCTCGAGG ACACAGAGGA AGAGAGAGGA 3841 GTGACCATGG ATCCACCAGT GAGTGAGGAG CGATCGGTGC AGCAGAGCCA CCCCACTACC 3901 ACCACCTCAC CCCCCCGGCC TTACCCAGAG CTCATCTCTC GCCCCTCCCC ACCTACCTTC 3961 CACCGGTTCC TGCCAGACTT GCCCCCATCC CGAAGTGCAG TGGAGATCGC CCCCACTCAG 4021 GTCACAGAGA CCGATGAGTG CCGATTGAAC CAGAATATCT GTGGCCATGG ACAGTGTGTG 4081 CCTGGCCCCT CGGATTACTC CTGCCACTGC AACGCTGGCT ACCGGTCACA CCCGCAGCAC 4141 CGCTACTGTG TTGATGTGAA CGAGTGCGAG GCAGAGCCCT GCGGCCCCGG GAAAGGCATC 4201 TGTATGAACA CTGGTGGCTC CTACAATTGT CACTGCAACC GAGGCTACCG CCTCCACGTG 4261 GGTGCAGGGG GCCGCTCGTG CGTGGACCTG AACGAGTGCA CCAAGCCTCA CCTGTGTGGG 4321 GACGGTGGCT TCTGCATCAA CTTCCCTGGT CACTACAAAT GCAACTGCTA TCCTGGCTAC 4381 CGGCTCAAGG CCTCCCGACC GCCCATTTGC GAAGACATCG ACGAGTGTCG CGACCCTAGC 4441 ACCTGCCCTG ATGGCAAATG TGAAAACAAA CCTGGCAGCT TCAAGTGCAT CGCCTGCCAG 4501 CCTGGCTACC GTAGCCAGGG GGGCGGGGCC TGTCGTGATG TCAACGAATG CTCCGAGGGT 4561 ACCCCCTGCT CTCCTGGATG GTGTGAGAAC CTTCCGGGTT CTTACCGTTG CACGTGTGCC 4621 CAGGGATACG AACCCGCACA GGACGGCCTC AGTTGCATAG ACGTGGATGA GTGTGAGGCT 4681 GGGAAAGTGT GCCAAGATGG CATCTGCACG AACACACCAG GCTCTTTCCA GTGTCAGTGC 4741 CTCTCCGGCT ATCATCTGTC AAGGGATCGG AGCCGCTGTG AGGACATTGA TGAATGTGAC 4801 TTCCCTGCGG CCTGCATCGG GGGTGACTGC ATCAATACCA ATGGTTCCTA CAGATGTCTC 4861 TGTCCCCAGG GTCATCGGTT GGTGGGCGGT AGGAAGTGCC AAGATATAGA TGAGTGCAGC 4921 CAGGACCCAG GCCTGTGCCT GCCCCACGGG GCCTGCGAGA ACCTCCAGGG CTCCTATGTC 4981 TGTGTCTGTG ATGAGGGTTT CACACTCACC CAGGACCAGC ATGGGTGTGA GGAGGTGGAG 5041 CAGCCCCACC ACAAGAAGGA GTGCTACCTT AACTTCGATG ACACAGTGTT CTGTGACAGC 5101 GTATTGGCTA CCAATGTCAC TCAGCAGGAA TGCTGTTGCT CTCTGGGAGC TGGTTGGGGA 5161 GACCACTGCG AAATCTATCC CTGTCCAGTC TACAGCTCAG CCGAATTTCA CAGCCTGTGT 5221 CCTGATGGGA AAGGCTACAC TCAGGACAAC AACATTGTGA ACTATGGCAT TCCTGCCCAC 5281 CGTGACATCG ACGAATGCAT ATTGTTTGGG GCAGAGATCT GCAAGGAGGG CAAGTGTGTG 5341 AACACGCAGC CCGGCTACGA GTGCTACTGC AAGCAGGGCT TCTACTACGA TGGCAACCTG 5401 CTGGAGTGCG TGGACGTGGA TGAGTGCTTG GATGAGTCTA ACTGCAGGAA CGGAGTGTGT 5461 GAGAACACAC GTGGCGGCTA CCGCTGTGCC TGCACGCCGC CGGCAGAGTA CAGTCCCGCA 5521 CAGCGCCAGT GTCTGAGCCC GGAGGAGATG GAGCACGCCC CAGAGAGACG TGAAGTGTGC 5581 TGGGGCCAGC GAGGAGAGGA CGGCATGTGT ATGGGGCCCC TGGCGGGACC TGCCCTCACT 5641 TTTGATGACT GCTGCTGCCG CCAGGGCCGC GGCTGGGGTA CCCAGTGCAG ACCGTGCCCG 5701 CCACGTGGCA CCGGGTCCCA GTGCCCGACT TCACAGAGTG AGAGCAATTC ATTCTGGGAC 5761 ACAAGCCCCC TGCTACTGGG GAAGTCTCCG CGAGACGAAG ACAGCTCAGA GGAGGATTCA 5821 GATGAGTGCC GTTGTGTGAG CGGACGCTGT GTGCCACGGC CAGGCGGGGC GGTATGCGAG 5881 TGTCCTGGAG GCTTTCAGCT GGACGCCTCC CGTGCCCGCT GCGTGGACAT TGATGAGTGC 5941 CGAGAACTGA ACCAGCGGGG ACTACTGTGT AAGAGCGAGC GGTGCGTGAA CACCAGTGGA 6001 TCCTTCCGCT GTGTCTGCAA AGCTGGCTTC ACGCGCAGCC GCCCTCACGG GGCCTGCGTG 6061 CCTCAGCGCC GCCGCggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 6121 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 6181 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 6241 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 6301 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 6361 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 6421 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 6481 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 6541 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 6601 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 6661 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 6721 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 6781 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 6841 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 6901 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 6961 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 7021 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 7081 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 7141 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 7201 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 7261 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 7321 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 7381 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 7441 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 7501 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 7561 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 7621 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 7681 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 7741 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 7801 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 7861 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 7921 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 7981 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 8041 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 8101 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 8161 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 8221 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 8281 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 8341 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 8401 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 8461 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 8521 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 8581 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 8641 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 8701 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 8761 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 8821 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 8881 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 8941 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 9001 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 9061 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 9121 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 9181 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 9241 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 9301 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 9361 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 9421 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 9481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 9541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 9601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 9661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 9721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 9781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 9841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 9901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 9961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 10021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 10081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 10141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 10201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 10261 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 10321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 10381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 10441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 10501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 10561 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 10621 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 10681 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 10741 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 10801 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 10861 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 10921 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 10981 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 11041 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 11101 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 11161 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 11221 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 11281 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 11341 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 11401 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 11461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 11521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 11581 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 11641 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 11701 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 11761 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 11821 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 11881 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 11941 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 12001 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.