pCMV-TSC1-3×Myc-Neo plasmid

pCMV-TSC1-3×Myc-Neo plasmid

PVT95372

pCMV-TSC1-3×Myc-Neo plasmid Information

Plasmid name: pCMV-TSC1(human)-3×Myc-Neo
Alias: NM_000368.5;LAM; TSC
Gene lenght: 3492bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TSC1-3×Myc-Neo plasmid Description

pCMV-TSC1-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TSC1-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCg ccaccatggc ccaacaagca 721 aatgtcgggg agcttcttgc catgctggac tcccccatgc tgggtgtgcg ggacgacgtg 781 acagctgtct ttaaagagaa cctcaattct gaccgtggcc ctatgcttgt aaacaccttg 841 gtggattatt acctggaaac cagctctcag ccggcattgc acatcctgac caccttgcaa 901 gagccacatg acaagcacct cttggacagg attaacgaat atgtgggcaa agccgccact 961 cgtttatcca tcctctcgtt actgggtcat gtcataagac tgcagccatc ttggaagcat 1021 aagctctctc aagcacctct tttgccttct ttactaaaat gtctcaagat ggacactgac 1081 gtcgttgtcc tcacaacagg cgtcttggtg ttgataacca tgctaccaat gattccacag 1141 tctgggaaac agcatcttct tgatttcttt gacatttttg gccgtctgtc atcatggtgc 1201 ctgaagaaac caggccacgt ggcggaagtc tatctcgtcc atctccatgc cagtgtgtac 1261 gcactctttc atcgccttta tggaatgtac ccttgcaact tcgtctcctt tttgcgttct 1321 cattacagta tgaaagaaaa cctggagact tttgaagaag tggtcaagcc aatgatggag 1381 catgtgcgaa ttcatccgga attagtgact ggatccaagg accatgaact ggaccctcga 1441 aggtggaaga gattagaaac tcatgatgtt gtgatcgagt gtgccaaaat ctctctggat 1501 cccacagaag cctcatatga agatggctat tctgtgtctc accaaatctc agcccgcttt 1561 cctcatcgtt cagccgatgt caccaccagc ccttatgctg acacacagaa tagctatggg 1621 tgtgctactt ctacccctta ctccacgtct cggctgatgt tgttaaatat gccagggcag 1681 ctacctcaga ctctgagttc cccatcgaca cggctgataa ctgaaccacc acaagctact 1741 ctttggagcc catctatggt ttgtggtatg accactcctc caacttctcc tggaaatgtc 1801 ccacctgatc tgtcacaccc ttacagtaaa gtctttggta caactgcagg tggaaaagga 1861 actcctctgg gaaccccagc aacctctcct cctccagccc cactctgtca ttcggatgac 1921 tacgtgcaca tttcactccc ccaggccaca gtcacacccc ccaggaagga agagagaatg 1981 gattctgcaa gaccatgtct acacagacaa caccatcttc tgaatgacag aggatcagaa 2041 gagccacctg gcagcaaagg ttctgtcact ctaagtgatc ttccagggtt tttaggtgat 2101 ctggcctctg aagaagatag tattgaaaaa gataaagaag aagctgcaat atctagagaa 2161 ctttctgaga tcaccacagc agaggcagag cctgtggttc ctcgaggagg ctttgactct 2221 cccttttacc gagacagtct cccaggttct cagcggaaga cccactcggc agcctccagt 2281 tctcagggcg ccagcgtgaa ccctgagcct ttacactcct ccctggacaa gcttgggcct 2341 gacacaccaa agcaagcctt tactcccata gacctgccct gcggcagtgc tgatgaaagc 2401 cctgcgggag acagggaatg ccagacttct ttggagacca gtatcttcac tcccagtcct 2461 tgtaaaattc cacctccgac gagagtgggc tttggaagcg ggcagcctcc cccgtatgat 2521 catctttttg aggtggcatt gccaaagaca gcccatcatt ttgtcatcag gaagactgag 2581 gagctgttaa agaaagcaaa aggaaacaca gaggaagatg gtgtgccctc tacctcccca 2641 atggaagtgc tggacagact gatacagcag ggagcagacg cgcacagcaa ggagctgaac 2701 aagttgcctt tacccagcaa gtctgtcgac tggacccact ttggaggctc tcctccttca 2761 gatgagatcc gcaccctccg agaccagttg cttttactgc acaaccagtt actctatgag 2821 cgttttaaga ggcagcagca tgccctccgg aacaggcggc tcctccgcaa ggtgatcaaa 2881 gcagcagctc tggaggaaca taatgctgcc atgaaagatc agttgaagtt acaagagaag 2941 gacatccaga tgtggaaggt tagtctgcag aaagaacaag ctagatacaa tcagctccag 3001 gagcagcgtg acactatggt aaccaagctc cacagccaga tcagacagct gcagcatgac 3061 cgagaggaat tctacaacca gagccaggaa ttacagacga agctggagga ctgcaggaac 3121 atgattgcgg agctgcggat agaactgaag aaggccaaca acaaggtgtg tcacactgag 3181 ctgctgctca gtcaggtttc ccaaaagctc tcaaacagtg agtcggtcca gcagcagatg 3241 gagttcttga acaggcagct gttggttctt ggggaggtca acgagctcta tttggaacaa 3301 ctgcagaaca agcactcaga taccacaaag gaagtagaaa tgatgaaagc cgcctatcgg 3361 aaagagctag aaaaaaacag aagccatgtt ctccagcaga ctcagaggct tgatacctcc 3421 caaaaacgga ttttggaact ggaatctcac ctggccaaga aagaccacct tcttttggaa 3481 cagaagaaat atctagagga tgtcaaactc caggcaagag gacagctgca ggccgcagag 3541 agcaggtatg aggctcagaa aaggataacc caggtgtttg aattggagat cttagattta 3601 tatggcaggt tggagaaaga tggcctcctg aaaaaacttg aagaagaaaa agcagaagca 3661 gctgaagcag cagaagaaag gcttgactgt tgtaatgacg ggtgctcaga ttccatggta 3721 gggcacaatg aagaggcatc tggccacaac ggtgagacca agacccccag gcccagcagc 3781 gcccggggca gtagtggaag cagaggtggt ggaggcagca gcagcagcag cagcgagctt 3841 tctaccccag agaaaccccc acaccagagg gcaggcccat tcagcagtcg gtgggagacg 3901 actatgggag aagcgtctgc cagcatcccc accactgtgg gctcacttcc cagttcaaaa 3961 agcttcctgg gtatgaaggc tcgagagtta tttcgtaata agagcgagag ccagtgtgat 4021 gaggacggca tgaccagtag cctttctgag agcctaaaga cagaactggg caaagacttg 4081 ggtgtggaag ccaagattcc cctgaaccta gatggccctc acccgtctcc cccgaccccg 4141 gacagtgttg gacagctaca tatcatggac tacaatgaga ctcatcatga acacagcCTC 4201 GAGGAACAAA AACTAATATC AGAGGAAGAT TTGGAACAGA AACTAATCAG TGAAGAAGAT 4261 TTAGAGCAAA AGTTAATTTC CGAGGAGGAC TTATAGTGAG GGCCCGATAT CTCGACAATC 4321 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT 4381 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG 4441 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC 4501 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT 4561 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG 4621 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG 4681 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT 4741 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC 4801 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC 4861 TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG 4921 GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA 4981 AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC 5041 AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT 5101 CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG 5161 TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC 5221 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 5281 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 5341 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA 5401 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 5461 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 5521 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 5581 AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 5641 ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC 5701 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC 5761 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT 5821 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC 5881 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA 5941 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC 6001 GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT 6061 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG 6121 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC 6181 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA 6241 GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT 6301 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA 6361 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG 6421 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT 6481 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA 6541 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG 6601 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG 6661 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT 6721 AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT 6781 CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA 6841 CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC 6901 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 6961 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 7021 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 7081 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 7141 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 7201 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 7261 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 7321 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 7381 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 7441 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 7501 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 7561 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 7621 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 7681 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 7741 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT 7801 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 7861 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 7921 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 7981 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 8041 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 8101 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 8161 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 8221 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 8281 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 8341 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 8401 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 8461 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 8521 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 8581 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 8641 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 8701 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 8761 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 8821 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 8881 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 8941 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 9001 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 9061 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 9121 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 9181 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 9241 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 9301 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG 9361 GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT CTGATGCCGC 9421 ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG 9481 CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG 9541 G

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.