pCMV-TSC1-3×HA-Neo plasmid

pCMV-TSC1-3×HA-Neo plasmid

PVT94860

pCMV-TSC1-3×HA-Neo plasmid Information

Plasmid name: pCMV-TSC1(human)-3×HA-Neo
Alias: NM_000368.5;LAM; TSC
Gene lenght: 3492bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TSC1-3×HA-Neo plasmid Description

pCMV-TSC1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TSC1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc ccaacaagca 721 aatgtcgggg agcttcttgc catgctggac tcccccatgc tgggtgtgcg ggacgacgtg 781 acagctgtct ttaaagagaa cctcaattct gaccgtggcc ctatgcttgt aaacaccttg 841 gtggattatt acctggaaac cagctctcag ccggcattgc acatcctgac caccttgcaa 901 gagccacatg acaagcacct cttggacagg attaacgaat atgtgggcaa agccgccact 961 cgtttatcca tcctctcgtt actgggtcat gtcataagac tgcagccatc ttggaagcat 1021 aagctctctc aagcacctct tttgccttct ttactaaaat gtctcaagat ggacactgac 1081 gtcgttgtcc tcacaacagg cgtcttggtg ttgataacca tgctaccaat gattccacag 1141 tctgggaaac agcatcttct tgatttcttt gacatttttg gccgtctgtc atcatggtgc 1201 ctgaagaaac caggccacgt ggcggaagtc tatctcgtcc atctccatgc cagtgtgtac 1261 gcactctttc atcgccttta tggaatgtac ccttgcaact tcgtctcctt tttgcgttct 1321 cattacagta tgaaagaaaa cctggagact tttgaagaag tggtcaagcc aatgatggag 1381 catgtgcgaa ttcatccgga attagtgact ggatccaagg accatgaact ggaccctcga 1441 aggtggaaga gattagaaac tcatgatgtt gtgatcgagt gtgccaaaat ctctctggat 1501 cccacagaag cctcatatga agatggctat tctgtgtctc accaaatctc agcccgcttt 1561 cctcatcgtt cagccgatgt caccaccagc ccttatgctg acacacagaa tagctatggg 1621 tgtgctactt ctacccctta ctccacgtct cggctgatgt tgttaaatat gccagggcag 1681 ctacctcaga ctctgagttc cccatcgaca cggctgataa ctgaaccacc acaagctact 1741 ctttggagcc catctatggt ttgtggtatg accactcctc caacttctcc tggaaatgtc 1801 ccacctgatc tgtcacaccc ttacagtaaa gtctttggta caactgcagg tggaaaagga 1861 actcctctgg gaaccccagc aacctctcct cctccagccc cactctgtca ttcggatgac 1921 tacgtgcaca tttcactccc ccaggccaca gtcacacccc ccaggaagga agagagaatg 1981 gattctgcaa gaccatgtct acacagacaa caccatcttc tgaatgacag aggatcagaa 2041 gagccacctg gcagcaaagg ttctgtcact ctaagtgatc ttccagggtt tttaggtgat 2101 ctggcctctg aagaagatag tattgaaaaa gataaagaag aagctgcaat atctagagaa 2161 ctttctgaga tcaccacagc agaggcagag cctgtggttc ctcgaggagg ctttgactct 2221 cccttttacc gagacagtct cccaggttct cagcggaaga cccactcggc agcctccagt 2281 tctcagggcg ccagcgtgaa ccctgagcct ttacactcct ccctggacaa gcttgggcct 2341 gacacaccaa agcaagcctt tactcccata gacctgccct gcggcagtgc tgatgaaagc 2401 cctgcgggag acagggaatg ccagacttct ttggagacca gtatcttcac tcccagtcct 2461 tgtaaaattc cacctccgac gagagtgggc tttggaagcg ggcagcctcc cccgtatgat 2521 catctttttg aggtggcatt gccaaagaca gcccatcatt ttgtcatcag gaagactgag 2581 gagctgttaa agaaagcaaa aggaaacaca gaggaagatg gtgtgccctc tacctcccca 2641 atggaagtgc tggacagact gatacagcag ggagcagacg cgcacagcaa ggagctgaac 2701 aagttgcctt tacccagcaa gtctgtcgac tggacccact ttggaggctc tcctccttca 2761 gatgagatcc gcaccctccg agaccagttg cttttactgc acaaccagtt actctatgag 2821 cgttttaaga ggcagcagca tgccctccgg aacaggcggc tcctccgcaa ggtgatcaaa 2881 gcagcagctc tggaggaaca taatgctgcc atgaaagatc agttgaagtt acaagagaag 2941 gacatccaga tgtggaaggt tagtctgcag aaagaacaag ctagatacaa tcagctccag 3001 gagcagcgtg acactatggt aaccaagctc cacagccaga tcagacagct gcagcatgac 3061 cgagaggaat tctacaacca gagccaggaa ttacagacga agctggagga ctgcaggaac 3121 atgattgcgg agctgcggat agaactgaag aaggccaaca acaaggtgtg tcacactgag 3181 ctgctgctca gtcaggtttc ccaaaagctc tcaaacagtg agtcggtcca gcagcagatg 3241 gagttcttga acaggcagct gttggttctt ggggaggtca acgagctcta tttggaacaa 3301 ctgcagaaca agcactcaga taccacaaag gaagtagaaa tgatgaaagc cgcctatcgg 3361 aaagagctag aaaaaaacag aagccatgtt ctccagcaga ctcagaggct tgatacctcc 3421 caaaaacgga ttttggaact ggaatctcac ctggccaaga aagaccacct tcttttggaa 3481 cagaagaaat atctagagga tgtcaaactc caggcaagag gacagctgca ggccgcagag 3541 agcaggtatg aggctcagaa aaggataacc caggtgtttg aattggagat cttagattta 3601 tatggcaggt tggagaaaga tggcctcctg aaaaaacttg aagaagaaaa agcagaagca 3661 gctgaagcag cagaagaaag gcttgactgt tgtaatgacg ggtgctcaga ttccatggta 3721 gggcacaatg aagaggcatc tggccacaac ggtgagacca agacccccag gcccagcagc 3781 gcccggggca gtagtggaag cagaggtggt ggaggcagca gcagcagcag cagcgagctt 3841 tctaccccag agaaaccccc acaccagagg gcaggcccat tcagcagtcg gtgggagacg 3901 actatgggag aagcgtctgc cagcatcccc accactgtgg gctcacttcc cagttcaaaa 3961 agcttcctgg gtatgaaggc tcgagagtta tttcgtaata agagcgagag ccagtgtgat 4021 gaggacggca tgaccagtag cctttctgag agcctaaaga cagaactggg caaagacttg 4081 ggtgtggaag ccaagattcc cctgaaccta gatggccctc acccgtctcc cccgaccccg 4141 gacagtgttg gacagctaca tatcatggac tacaatgaga ctcatcatga acacagcCTC 4201 GAGTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC TGATTATGCT 4261 AGCTATCCTT ATGATGTCCC AGACTACGCA TAGTGAGGGC CCGATATCTC GACAATCAAC 4321 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 4381 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 4441 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4501 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4561 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4621 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4681 CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG 4741 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4801 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4861 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT 4921 AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG 4981 ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG 5041 GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT 5101 CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG 5161 GGGCGGCAGG CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG 5221 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 5281 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 5341 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG 5401 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 5461 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 5521 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 5581 GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 5641 CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC 5701 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC 5761 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT 5821 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 5881 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT 5941 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG 6001 AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT 6061 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA 6121 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA 6181 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA 6241 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA 6301 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT 6361 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG 6421 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA 6481 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA 6541 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA 6601 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG 6661 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC 6721 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT 6781 GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA 6841 GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC 6901 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 6961 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 7021 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 7081 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 7141 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 7201 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 7261 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 7321 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 7381 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 7441 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 7501 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 7561 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 7621 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 7681 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 7741 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC 7801 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 7861 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 7921 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 7981 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 8041 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 8101 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 8161 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 8221 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 8281 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 8341 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 8401 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 8461 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 8521 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 8581 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 8641 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 8701 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 8761 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 8821 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 8881 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 8941 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 9001 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 9061 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 9121 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 9181 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 9241 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 9301 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT 9361 CGGGAGATCT CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA 9421 GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA 9481 AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.