pCAG-FMNL1-3×FLAG-Neo plasmid

pCAG-FMNL1-3×FLAG-Neo plasmid

PVT95164

pCAG-FMNL1-3×FLAG-Neo plasmid Information

Plasmid name: pCAG-FMNL1(human)-3×FLAG-Neo
Alias: NM_001411128.1;C17orf1; C17orf1B; FHOD4; FMNL; KW-13
Gene lenght: 3312bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pCAG-FMNL1-3×FLAG-Neo plasmid Description

pCAG-FMNL1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCAG-FMNL1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCtacgtatt agtcatcgct 421 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 481 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 541 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 601 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 661 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg 721 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga 781 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat 841 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg 901 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc 961 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc 1021 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg 1081 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg 1141 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga 1201 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc 1261 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg 1321 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg 1381 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc 1441 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg 1501 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc 1561 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg 1621 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc 1681 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt 1741 gctggttatt gtgctgtctc atcattttgg caaaGAATTC GCCACCatgg gcaacgcggc 1801 cggcagcgcc gagcagcccg cgggccccgc cgcgccgccc cccaagcagc ccgcgcctcc 1861 caagcagccg atgcccgcgg ccggagagct ggaggagagg ttcaaccgcg ccctgaactg 1921 catgaacttg cccccagaca aggtccagct gctgagccag tatgacaacg agaagaagtg 1981 ggagctcatc tgtgatcagg agcggtttca agtcaagaat ccccccgcag cctacatcca 2041 gaagctgaag agctatgtgg atactggtgg ggtcagccga aaggtagcag ctgattggat 2101 gtccaacctg gggtttaaga ggcgagttca ggagtccacg caggtgctac gggagctgga 2161 gacctccctg aggaccaacc acattgggtg ggtgcaggag ttcctcaatg aagagaaccg 2221 tggcctggat gtgctgctcg agtacctggc ctttgcccag tgctctgtca cgtatgacat 2281 ggagagcaca gacaacgggg cttccaactc agagaaaaac aagcccctgg agcagtctgt 2341 ggaagacctc agcaagggtc caccctcctc cgtgcccaaa agccgccacc tgaccatcaa 2401 gctgacccca gcccacagca ggaaggccct gcggaattcc cgcatcgtca gccagaagga 2461 cgacgtccac gtctgtatta tgtgcctacg cgccatcatg aactaccagt ctggcttcag 2521 ccttgtcatg aaccacccag cctgtgtcaa tgagattgct ctgagcctca acaacaagaa 2581 ccccagaacc aaggctctgg tgctggagct gctggcggcc gtgtgcttgg tgcggggagg 2641 acatgacatc atccttgcag cctttgacaa cttcaaggag gtgtgtgggg agcagcaccg 2701 ctttgaaaag ctgatggaat atttccggaa tgaggacagc aacatcgact tcatggtggc 2761 ctgcatgcag ttcatcaaca ttgtggtaca ttcggtggag aacatgaact tccgtgtctt 2821 cctgcaatat gagttcaccc acttgggcct ggacctgtac ttggagaggc ttcggctcac 2881 cgagagtgac aagctgcagg tgcagatcca ggcgtacctg gacaatattt ttgatgtggg 2941 ggcgctgctg gaggacacag agaccaagaa cgctgtgctg gagcacatgg aggaactgca 3001 ggagcaagtg gcgctgctga cagagcggct tcgggacgcg gagaacgaat ccatggccaa 3061 gattgcagaa ctggaaaaac agctaagcca ggcgcgcaag gagttggaga ccctgcggga 3121 gcgcttcagc gaatcgaccg ccatggggcc ctccaggcgt cccccagagc ctgagaaagc 3181 gcctcccgct gccccgacgc ggccctcggc cctggagctg aaggtggagg agctggagga 3241 gaaggggtta atccgtattc tgcgggggcc gggggatgct gtctccatcg agatcctccc 3301 cgtcgctgtg gcaactccga gcggcggtga tgctccgact ccgggggtgc cgaccggctc 3361 ccccagccca gatctcgcac ctgcagcaga gccggctccc ggagcagcgc caccgccgcc 3421 gcccccactg cccggcctcc cctccccgca ggaagccccg ccctctgcgc ccccacaggc 3481 cccgcctctc cctggcagcc cggagccccc gcctgcgccg ccgctgcccg gagacctgcc 3541 gcccccaccc ccgccaccgc caccacctcc gggcactgac gggccggtgc ctccgccgcc 3601 gccgccgccg ccgccgcctc ccggaggtcc tcctgatgcc ctaggaagac gcgactcaga 3661 attgggccca ggagtgaagg ccaagaagcc catccagact aagttccgaa tgccactctt 3721 gaactgggtg gcactgaaac ccagccagat caccggcact gtcttcacag agctcaatga 3781 tgagaaggtg ctgcaggagc tagacatgag tgattttgag gaacagttca agaccaagtc 3841 ccaaggcccc agcctggacc tcagcgctct caagagtaag gcagcccaga aggcccccag 3901 caaggcgaca ctcattgagg ccaaccgggc caagaacttg gccatcaccc tgcggaaggg 3961 caacctgggg gccgagcgca tctgccaagc cattgaggcg tacgacctgc aggctctggg 4021 cctggacttc ctggagctgc tgatgcgctt cctgcccaca gagtatgagc gcagcctcat 4081 cacccgcttt gagcgggagc agcggccaat ggaggagctg tcagaggagg accgcttcat 4141 gctatgcttc agccgcatcc cgcgcctgcc ggagcgcatg accacactca ccttcctggg 4201 caacttcccg gacacagccc agctgctcat gccgcaactg aatgccatca ttgcagcctc 4261 aatgtccatc aagtcctctg acaaactccg ccagatcctg gagattgtcc tggcctttgg 4321 caactacatg aacagtagca agcgtggggc agcctatggc ttccggctcc agagcctgga 4381 tgcgctgttg gagatgaagt cgactgatcg caagcagacg ctgctgcact acctggtgaa 4441 ggtcattgct gagaagtacc cgcaactcac aggcttccac agcgacctgc acttcctgga 4501 caaggcgggc tcagtgtccc tggacagtgt cctggcggac gtgcgctccc tgcagcgagg 4561 cctagagttg acacagagag agtttgtgcg gcaggatgac tgcatggtgc tcaaggagtt 4621 cctgagggcc aactcgccca ccatggacaa gctgctggca gacagcaaga cggctcagga 4681 ggcctttgag tctgtggtgg agtacttcgg agagaacccc aagaccacat ccccaggcct 4741 gttcttctcc ctctttagcc gcttcattaa ggcctacaag aaagctgagc aggaggtgga 4801 acagtggaaa aaagaagccg ctgcccagga ggcaggcgct gataccccgg gcaaagggga 4861 gcccccagca cccaagtcac cgccaaaggc ccggcggcca cagatggacc tcatctctga 4921 gctgaaacgg aggcagcaga aggagccact catttatgag agcgaccgtg atggggccat 4981 tgaagacatc atcacagatc tgcggaacca gccctacatc cgcgcagaca caggccgccg 5041 cagtgcccgt cggcgtcccc cgggcccccc actgcaggtc acctccgacc tctcgctgCT 5101 CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 5161 CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT 5221 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 5281 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 5341 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 5401 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 5461 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 5521 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 5581 TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 5641 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 5701 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 5761 TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA 5821 CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT 5881 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC 5941 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC 6001 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA 6061 GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA 6121 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 6181 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 6241 GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 6301 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 6361 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 6421 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC 6481 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG 6541 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 6601 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 6661 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 6721 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 6781 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 6841 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 6901 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 6961 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 7021 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 7081 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 7141 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 7201 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 7261 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 7321 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 7381 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 7441 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 7501 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 7561 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 7621 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 7681 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 7741 GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC 7801 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG 7861 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA 7921 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 7981 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA 8041 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT 8101 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG 8161 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG 8221 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 8281 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 8341 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 8401 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 8461 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 8521 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 8581 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 8641 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG 8701 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 8761 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 8821 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 8881 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 8941 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 9001 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 9061 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 9121 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 9181 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 9241 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 9301 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 9361 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 9421 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 9481 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 9541 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 9601 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 9661 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 9721 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 9781 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 9841 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 9901 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 9961 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 10021 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 10081 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 10141 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 10201 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC 10261 TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC 10321 CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC 10381 AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.