pSDPR-Fluc-SV40-hRluc-Neo plas

pSDPR-Fluc-SV40-hRluc-Neo plasmid

PVT95120

pSDPR-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pSDPR(human)-Fluc-SV40-hRluc-Neo
Alias:
Gene lenght: 1495bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: SDPR
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pSDPR-Fluc-SV40-hRluc-Neo plasmid Description

pSDPR-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pSDPR-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcT TAAGAGCAGC TGGAATGCAG TTCCCCTGAT CAGCGTAGCC AGTTGTTGCC 5161 TGTCTGAACC TCTGCCAGTC CTGGAGACTG GTGCCCTGAG CTCCAACCAG CGGGCCTCAT 5221 CCTACACCCT CACCACCGCA ACTTCTCACC CGAGCAAGAA GCAGCTCCCA GAGAGAAAGA 5281 ACGTTCCCAC CTGCCTAGCC ATGGGAGAGG ACGCTGCACA GGCCGAAAAG TTCCAGCACC 5341 CTGGGTCTGA CATGCGGCAG GAAAAGCCCT CGAGCCCCAG CCCGATGCCT TCCTCCACAC 5401 CAAGCCCCAG CCTGAACCTA GGGAACACAG AGGAGGCCAT CCGGGACAAC TCACAGGTGA 5461 ACGCAGTCAC GGTGCTCACG CTCCTGGACA AGCTGGTGAA CATGCTAGAC GCTGTGCAGG 5521 AGAACCAGCA CAAGATGGAG CAGCGACAGA TCAGTTTGGA GGGCTCCGTG AAGGGCATCC 5581 AGAATGACCT CACCAAGCTC TCCAAGTACC AGGCCTCCAC CAGCAACACG GTGAGCAAGC 5641 TGCTGGAGAA GTCCCGCAAG GTCAGCGCCC ACACGCGCGC GGTCAAAGAG CGCATGGATA 5701 GGCAGTGCGC ACAGGTGAAG CGGCTGGAGA ACAACCACGC CCAGCTCCTC CGACGCAACC 5761 ATTTCAAAGT GCTCATCTTC CAGGTCAGTT CCCCCTATGC CTCCTTACAG GCGGGCTGGA 5821 ACTCCTTATC ATTCTTGATC TCGCTCATCC TGGCTCTCAC AGCCATTTTT ACATTTGTCC 5881 TGCCTGTCAG GGATCACACA TACAAGGCAC TGTCCCCTGT CTGCCTGCGG AAAGCAAATG 5941 TGAAATCTTC ACACAGGGGT GGGAATTTCC CAGGTCACTA CAGACTAATC CAAAGAAGAT 6001 GTCCCCGGGC TTAGTAGGCC CCGGGTCATT TGTCAGGGAG CACCTGGATG CACTCTGTAC 6061 AGGTTGGGAA GATGTGCAGG TGCGTGCACC CTAGTCAAAT TCATTTGTCT CTTTGCATTA 6121 GGAGCTTAGG GCTCTGGCTG CAGCTGTTAG CTGGACAGGG CAAATTAATT CAGTGGCTGT 6181 TCATTAACTC TTAGTGGAAA TGAAGTGATC ATGTGAGGTA TTTTGTGGTT TTTTTAACCA 6241 AAAAGTCAAA CTATCCCTTT ATACAAAGGC TATCAACGTT TTGGGGACCA ATCAAATAGA 6301 GGATTGATTC CATTAGAGTT AGCCTTGTTC TTTTAAGACT TTTTTGGGGC AAAGCAATCT 6361 TGTGGAGTTC TTTTCTAGCA AATGCACTAG AAAAAGAGAG TACAAACACA CTAGACGTGC 6421 CTCTCCTTGT TCAGAGAGAC CACAACTGTT AAGTAAAATA AAAGGAGTGT TATCAGTTAT 6481 AGTCACAAAG GCTAGTCTTA AATGTTTCTC CTTCAAACGG TTGCCTTAGA ATAAAAAGGC 6541 CACTTAATTT TTTTTAATGC ATCAATGATG CAATTAATGT AGCAAGAGGC TATCTGGGGA 6601 CAGGggatcc CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC 6661 CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG 6721 TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT 6781 ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC 6841 ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC 6901 TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA 6961 ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA 7021 TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA 7081 CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT 7141 TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA 7201 TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT 7261 GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG 7321 CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT 7381 TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA 7441 GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG 7501 CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG 7561 GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA 7621 TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG 7681 ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT 7741 TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA 7801 TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG 7861 GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG 7921 ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA 7981 GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG 8041 ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA 8101 AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG 8161 TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC 8221 GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGTAATTC TAGTTGTTTA 8281 AACGAGCTCG CTAGCCTCGA GTCTAGAGTC GACCTGCAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.