pLV3-CMV-ABRAXAS2-3×HA-CopGFP-

pLV3-CMV-ABRAXAS2-3×HA-CopGFP-Puro plasmid

PVT95089

pLV3-CMV-ABRAXAS2-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ABRAXAS2(human)-3×HA-CopGFP-Puro
Alias: NM_032182.4;ABRO1; FAM175B; KIAA0157
Gene lenght: 1245bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ABRAXAS2-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-ABRAXAS2-3×HA-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ABRAXAS2-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cggcgtccat ttcgggctac 2341 accttcagtg ctgtgtgttt ccacagcgcc aacagcaacg cggaccacga aggattttta 2401 ctgggagagg taagacaaga ggaaacgttt agcatcagtg actcacaaat cagcaacaca 2461 gaatttctgc aagtaattga aatccataac catcagcctt gttcaaaact ttttagtttt 2521 tatgactacg caagcaaagt gaatgaggag agtttggaca ggattcttaa agatcggaga 2581 aagaaagtca ttgggtggta cagattccgg cgcaatacgc agcagcagat gtcctacaga 2641 gagcaggttc ttcacaagca gctcacccgc atcctcggcg tgcccgacct cgtctttctt 2701 ctcttcagct tcatctccac tgccaacaat tccactcacg ctttagaata tgtgctcttc 2761 agaccaaata gaaggtataa tcagaggata tcactcgcta ttcccaatct aggaaatact 2821 agccagcaag agtacaaagt gtcttcagtg ccaaatactt ctcagagtta tgccaaagtg 2881 attaaagaac atggtactga cttttttgac aaggatggag tgatgaaaga catcagggcg 2941 atttatcagg tttataatgc acttcaggag aaagttcagg cagtgtgtgc agatgttgaa 3001 aagagtgagc gagttgttga atcttgtcag gcagaagtga acaaattaag aagacaaatc 3061 actcagagga aaaatgaaaa ggaacaagaa agaagattgc agcaggcagt gttaagcaga 3121 cagatgccgt ctgaaagctt ggacccagcg ttcagtcctc ggatgccgtc ctctgggttt 3181 gcagctgaag gcagaagtac acttggagat gcagaggcct cggatcctcc tcccccttac 3241 tctgattttc acccaaacaa tcaagaaagt actttgagcc actctcgcat ggaaaggagt 3301 gtctttatgc ctcgacctca agctgtgggc tcttccaatt atgcttccac cagtgccgga 3361 ctgaagtatc ctggaagtgg ggctgacctt cctcctcccc aaagagcagc tggagacagt 3421 ggtgaggatt cagacgacag tgattatgaa aatttgattg accctacaga gccttctaat 3481 agtgaatact cacattcaaa ggattctcga cccatggcac atcccgacga ggaccccagg 3541 aacactcaga cctcccagat tCTCGAGTAC CCATACGACG TACCTGATTA TGCAACTTAT 3601 CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATAGgga 3661 tccCTGCGAT ATCCAGCgcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3721 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3781 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3841 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3901 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3961 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 4021 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 4081 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 4141 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 4201 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 4261 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 4321 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 4381 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 4441 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 4501 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 4561 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 4621 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 4681 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4741 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4801 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4861 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4921 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4981 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 5041 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5101 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5161 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5221 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5281 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5341 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5401 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5461 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5521 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5581 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5641 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 6001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 6061 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6181 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc 6241 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6301 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt 6361 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 6421 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 6481 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 6541 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 6601 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 6661 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 6721 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 6781 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 6841 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 6901 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt 6961 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 7021 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 7081 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 7141 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7741 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 7801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7981 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 8041 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 8101 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 8161 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8221 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8281 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8341 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8401 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8461 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8521 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8581 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8641 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8701 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8761 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8821 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8881 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8941 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 9001 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 9061 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 9121 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 9181 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 9241 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 9301 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 9361 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 9421 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 9481 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 9541 cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.