pPGK-SLC43A1-Fluc-SV40-hRluc-N

pPGK-SLC43A1-Fluc-SV40-hRluc-Neo plasmid

PVT94907

pPGK-SLC43A1-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pPGK-SLC43A1(human)-Fluc-SV40-hRluc-Neo
Alias: NM_003627.6;LAT3; PB39; POV1; R00504
Gene lenght: 1680bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: SLC43A1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pPGK-SLC43A1-Fluc-SV40-hRluc-Neo plasmid Description

pPGK-SLC43A1-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pPGK-SLC43A1-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattca ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 5161 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 5221 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 5281 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 5341 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 5401 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 5461 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 5521 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 5581 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagcttg gcaatccggt 5641 actgttggta aaggccacca tggcccccac gctgcaacag gcgtaccgga ggcgctggtg 5701 gatggcctgc acggctgtgc tggagaacct cttcttctct gctgtactcc tgggctgggg 5761 ctccctgttg atcattctga agaacgaggg cttctattcc agcacgtgcc cagctgagag 5821 cagcaccaac accacccagg atgagcagcg caggtggcca ggctgtgacc agcaggacga 5881 gatgctcaac ctgggcttca ccattggttc cttcgtgctc agcgccacca ccctgccact 5941 ggggatcctc atggaccgct ttggcccccg acccgtgcgg ctggttggca gtgcctgctt 6001 cactgcgtcc tgcaccctca tggccctggc ctcccgggac gtggaagctc tgtctccgtt 6061 gatattcctg gcgctgtccc tgaatggctt tggtggcatc tgcctaacgt tcacttcact 6121 cacgctgccc aacatgtttg ggaacctgcg ctccacgtta atggccctca tgattggctc 6181 ttacgcctct tctgccatta cgttcccagg aatcaagctg atctacgatg ccggtgtggc 6241 cttcgtggtc atcatgttca cctggtctgg cctggcctgc cttatctttc tgaactgcac 6301 cctcaactgg cccatcgaag cctttcctgc ccctgaggaa gtcaattaca cgaagaagat 6361 caagctgagt gggctggccc tggaccacaa ggtgacaggt gacctcttct acacccatgt 6421 gaccaccatg ggccagaggc tcagccagaa ggcccccagc ctggaggacg gttcggatgc 6481 cttcatgtca ccccaggatg ttcggggcac ctcagaaaac cttcctgaga ggtctgtccc 6541 cttacgcaag agcctctgct cccccacttt cctgtggagc ctcctcacca tgggcatgac 6601 ccagctgcgg atcatcttct acatggctgc tgtgaacaag atgctggagt accttgtgac 6661 tggtggccag gagcatgaga caaatgaaca gcaacaaaag gtggcagaga cagttgggtt 6721 ctactcctcc gtcttcgggg ccatgcagct gttgtgcctt ctcacctgcc ccctcattgg 6781 ctacatcatg gactggcgga tcaaggactg cgtggacgcc ccaactcagg gcactgtcct 6841 cggagatgcc agggacgggg ttgctaccaa atccatcaga ccacgctact gcaagatcca 6901 aaagctcacc aatgccatca gtgccttcac cctgaccaac ctgctgcttg tgggttttgg 6961 catcacctgt ctcatcaaca acttacacct ccagtttgtg acctttgtcc tgcacaccat 7021 tgttcgaggt ttcttccact cagcctgtgg gagtctctat gctgcagtgt tcccatccaa 7081 ccactttggg acgctgacag gcctgcagtc cctcatcagt gctgtgttcg ccttgcttca 7141 gcagccactt ttcatggcga tggtgggacc cctgaaagga gagcccttct gggtgaatct 7201 gggcctcctg ctattctcac tcctgggatt cctgttgcct tcctacctct tctattaccg 7261 tgcccggctc cagcaggagt acgccgccaa tgggatgggc ccactgaagg tgcttagcgg 7321 ctctgaggtg accgcaggtg gaggcggatc aggaggcggt ggatcggatc cCACCATGGA 7381 AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC 7441 CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT 7501 TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG 7561 GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG 7621 CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT 7681 GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA 7741 GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA 7801 GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA 7861 AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT 7921 GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC 7981 CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC 8041 CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC 8101 ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT 8161 CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT 8221 TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA 8281 CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA 8341 GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT 8401 GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT 8461 AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT 8521 GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT 8581 TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA 8641 CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT 8701 CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC 8761 CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC 8821 CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT 8881 GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT 8941 GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC 9001 CAAGAAGGGC GGCAAGATCG CCGTGTAATT CTAGTTGTTT AAACGAGCTC GCTAGCCTCG 9061 AGTCTAGAGT CGACCTGCAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.