pLV3-CMV-FPR2-3×FLAG-CopGFP-Pu

pLV3-CMV-FPR2-3×FLAG-CopGFP-Puro plasmid

PVT94842

pLV3-CMV-FPR2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FPR2(human)-3×FLAG-CopGFP-Puro
Alias: NM_001462.3;ALX; ALXR; FMLP-R-II; FMLPX; FPR2A; FPRH1;FPRH2; FPRL1; HM63; LXA4R
Gene lenght: 1053bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FPR2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FPR2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FPR2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aaaccaactt ctccactcct 2341 ctgaatgaat atgaagaagt gtcctatgag tctgctggct acactgttct gcggatcctc 2401 ccattggtgg tgcttggggt cacctttgtc ctcggggtcc tgggcaatgg gcttgtgatc 2461 tgggtggctg gattccggat gacacgcaca gtcaccacca tctgttacct gaacctggcc 2521 ctggctgact tttctttcac ggccacatta ccattcctca ttgtctccat ggccatggga 2581 gaaaaatggc cttttggctg gttcctgtgt aagttaattc acatcgtggt ggacatcaac 2641 ctctttggaa gtgtcttctt gattggtttc attgcactgg accgctgcat ttgtgtcctg 2701 catccagtct gggcccagaa ccaccgcact gtgagtctgg ccatgaaggt gatcgtcgga 2761 ccttggattc ttgctctagt ccttaccttg ccagttttcc tctttttgac tacagtaact 2821 attccaaatg gggacacata ctgtactttc aactttgcat cctggggtgg cacccctgag 2881 gagaggctga aggtggccat taccatgctg acagccagag ggattatccg gtttgtcatt 2941 ggctttagct tgccgatgtc cattgttgcc atctgctatg ggctcattgc agccaagatc 3001 cacaaaaagg gcatgattaa atccagccgt cccttacggg tcctcactgc tgtggtggct 3061 tctttcttca tctgttggtt tccctttcaa ctggttgccc ttctgggcac cgtctggctc 3121 aaagagatgt tgttctatgg caagtacaaa atcattgaca tcctggttaa cccaacgagc 3181 tccctggcct tcttcaacag ctgcctcaac cccatgcttt acgtctttgt gggccaagac 3241 ttccgagaga gactgatcca ctccctgccc accagtctgg agagggccct gtctgaggac 3301 tcagccccaa ctaatgacac ggctgccaat tctgcttcac ctcctgcaga gactgagtta 3361 caggcaatgg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC 3421 TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc cggtgcccgt 3481 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 3541 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 3601 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 3661 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 3721 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 3781 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 3841 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 3901 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 3961 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 4021 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 4081 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 4141 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 4201 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 4261 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 4321 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 4381 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 4441 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 4501 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 4561 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 4621 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 4681 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 4741 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 4801 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 4861 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 4921 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 4981 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 5041 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 5101 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 5161 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 5221 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 5281 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 5341 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 5401 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 5461 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5521 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5581 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 5641 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 5701 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 5761 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaATCA 5821 TCGTCCTTTC CTtggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 5881 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 5941 ctgcggcctc ttccgcgtct Tcgccttcgc cctcagacga gtcggatctc cctttggGcc 6001 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 6061 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa Gataagatct 6121 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 6181 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 6241 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 6301 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 6361 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 6421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6481 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctaT CCCGCCCCTA 6541 ACTCCGCCCA tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 6601 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 6661 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc 6721 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 6781 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 6841 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6901 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 7021 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 7081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 7141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 7201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 7261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 7321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 7381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 7441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 7501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 7561 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 7621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 7681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 7801 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 7861 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 7921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 8041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 8101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 8161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 8221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 8281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 8341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 8401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 8461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 8521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 8581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 8641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 8701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 8761 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 8821 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 8881 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 8941 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 9001 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 9061 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 9121 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 9181 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 9241 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 9301 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.