pLV2-CMV-SBSN-FLAG-Puro plasmi

pLV2-CMV-SBSN-FLAG-Puro plasmid

PVT88682

pLV2-CMV-SBSN-FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-SBSN(human-repeat-opt)-FLAG-Puro
Alias: NM_001166034.2;UNQ698
Gene lenght: 1770bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-SBSN-FLAG-Puro plasmid Description

pLV2-CMV-SBSN-FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-SBSN-FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 GCCACCATGC ATCTTGCACG TCTGGTCGGC TCCTGCTCCC TCCTTCTGCT ACTGGGGGCC 2881 CTGTCTGGAT GGGCGGCCAG CGATGACCCC ATTGAGAAGG TCATTGAAGG GATCAACCGA 2941 GGGCTGAGCA ATGCAGAGAG AGAGGTGGGC AAGGCCCTGG ATGGCATCAA CAGTGGAATC 3001 ACGCATGCCG GAAGGGAAGT GGAGAAGGTT TTCAACGGAC TTAGCAACAT GGGGAGCCAC 3061 ACCGGCAAGG AGTTGGACAA AGGCGTCCAG GGGCTCAACC ACGGCATGGA CAAGGTTGCC 3121 CATGAGATCA ACCATGGTAT TGGACAAGCA GGAAAGGAAG CAGAGAAGCT TGGCCATGGG 3181 GTCAACAACG CTGCTGGACA GGTTGGGAAG GAGGCAGACA AACTGATCCA TCATGGGGTC 3241 CATCACGGGG CCAACCAGGC CGGCTCTGAA GCCGGCAAGT TTGGACAGGG TGTGGACAAC 3301 GCCGCTGGAC AAGCTGGCAA CGAGGCCGGA AGATTCGGCC AGGGCGTTCA CCATGCTGCC 3361 GGCCAGGCAG GCAACGAAGC CGGAAGGTTC GGACAGGGAG TGCACCACGC AGCTGGACAG 3421 GCCGGCAATG AGGCTGGCAG GTTTGGCCAA GGCGCTCATC ACGGACTGAG CGAGGGATGG 3481 AAAGAGACGG AGAAGTTCGG ACAAGGCATC CACCACGCTG CCGGACAGGT GGGTAAAGAG 3541 GCAGAGAAGT TTGGCCAGGG CGCACATCAT GCCGCCGGAC AGGCTGGCAA TGAAGCTGGC 3601 AGATTTGGAC AAGGTGTGCA CCACGGCCTG AGCGAAGGTT GGAAAGAAAC AGAGAAATTC 3661 GGTCAAGGTG TCCACCACAC AGCTGGCCAG GTCGGCAAAG AGGCCGAGAA GTTCGGCCAG 3721 GGAGCACATC ACGCCGCTGG TCAGGCCGGA AATGAGGCCG GCAGATTCGG ACAGGGCGCT 3781 CACCATGCCG CAGGCCAGGC TGGAAACGAG GCAGGCAGGT TCGGCCAAGG CGTCCACCAC 3841 GGACTGTCTG AGGGCTGGAA AGAGACAGAG AAGTTCGGTC AGGGTGTGCA TCACGCTGCC 3901 TCTCAGTTCG GCAAAGAAAC CGAGAAGCTG GGACACGGAG TTCACCACGG AGTGAACGAG 3961 GCTTGGAAAG AGGCTGAGAA GTTTGGTCAG GGCGTGCACC ACGCCGCTTC TCAGGTGGGC 4021 AAAGAAGAAG ACAGGGTGGT CCAAGGCCTC CATCATGGCG TTAGTCAGGC TGGAAGGGAG 4081 GCGGGGCAGT TTGGCCACGA CATTCACCAC ACAGCAGGGC AGGCTGGGAA AGAGGGAGAC 4141 ATAGCAGTTC ATGGTGTCCA ACCTGGGGTC CACGAGGCCG GGAAGGAGGC AGGGCAGTTT 4201 GGCCAGGGAG TTCACCATAC CCTTGAACAG GCCGGGAAGG AAGCAGACAA AGCGGTCCAA 4261 GGGTTCCACA CTGGGGTCCA CCAGGCTGGG AAGGAAGCAG AGAAACTTGG CCAAGGGGTC 4321 AACCATGCTG CTGACCAGGC TGGAAAGGAA GTGGAGAAGC TTGGCCAAGG TGCCCACCAT 4381 GCTGCTGGCC AGGCCGGGAA GGAGCTGCAG AATGCTCATA ATGGGGTCAA CCAAGCCAGC 4441 AAGGAGGCCA ACCAGCTGCT GAATGGCAAC CATCAAAGCG GATCTTCCAG CCATCAAGGA 4501 GGGGCCACAA CCACGCCGTT AGCCTCTGGG GCCTCGGTCA ACACGCCTTT CATCAACCTT 4561 CCCGCCCTGT GGAGGAGCGT CGCCAACATC ATGCCCgaat tcGATTACAA GGATGACGAC 4621 GATAAGtgag gatcccgcga ctctagataa ttctaccggg taggggaggc gcttttccca 4681 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc 4741 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 4801 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag 4861 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg 4921 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt 4981 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg 5041 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 5101 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 5161 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 5221 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 5281 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 5341 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 5401 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 5461 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 5521 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 5581 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 5641 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 5701 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 5761 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa 5821 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5881 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5941 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6001 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6061 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6121 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6181 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 6241 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6301 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 6361 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag 6421 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 6481 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 6541 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 6601 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 6661 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 6721 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 6781 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 6841 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 6901 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 6961 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 7021 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 7081 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 7141 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7201 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 7261 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac 7321 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 7381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7501 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7561 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 8221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9481 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt 9541 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 9601 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 9661 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 9721 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 9781 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat 9841 ttgttaaata tgtactacaa acttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.