pLV3-CMV-EMX2-3×FLAG-EF1a-CopG

pLV3-CMV-EMX2-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94796

pLV3-CMV-EMX2-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-EMX2(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_004098.4
Gene lenght: 756bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EMX2-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-EMX2-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-EMX2-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgttcca gccggcgccc aagcgctgct tcaccatcga 2341 gtcgctggtg gccaaggaca gtcccctgcc cgcctcgcgc tccgaggacc ccatccgtcc 2401 cgcggcactc agctacgcta actccagccc cataaatccg ttcctcaacg gcttccactc 2461 ggccgccgcc gccgccgccg gtaggggcgt ctactccaac ccggacttgg tgttcgccga 2521 ggcggtctcg cacccgccca accccgccgt gccagtgcac ccggtgccgc cgccgcacgc 2581 cctggccgcc caccccctac cctcctcgca ctcgccacac cccctattcg cctcgcagca 2641 gcgggatccg tccaccttct acccctggct catccaccgc taccgatatc tgggtcatcg 2701 cttccaaggg aacgacacta gccccgagag tttccttttg cacaacgcgc tggcccgaaa 2761 gcccaagcgg atccgaaccg ccttctcccc gtcccagctt ctaaggctgg aacacgcctt 2821 tgagaagaat cactacgtgg tgggcgccga aaggaagcag ctggcacaca gcctcagcct 2881 cacggaaact caggtaaaag tatggtttca gaaccgaaga acaaagttca aaaggcagaa 2941 gctggaggaa gaaggctcag attcgcaaca aaagaaaaaa gggacgcacc atattaaccg 3001 gtggagaatc gccaccaagc aggcgagtcc ggaggaaata gacgtgacct cagatgatgg 3061 atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 3121 CGATGACAAG TAgcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 3181 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc 3241 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 3301 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 3361 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg 3421 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 3481 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag 3541 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct 3601 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta 3661 cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg 3721 cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga 3781 gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag 3841 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt 3901 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa 3961 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag 4021 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg 4081 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga 4141 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct 4201 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat 4261 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct 4321 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc 4381 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg 4441 ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa 4501 tcccggccct GCCACCatgg ctgaacaaga tggattgcac gcaggttctc cggccgcttg 4561 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 4621 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 4681 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 4741 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 4801 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 4861 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 4921 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 4981 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 5041 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 5101 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 5161 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 5221 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 5281 cttctatcgc cttcttgacg agttcttctg aGTCGACAAT CAACCTCTGG ATTACAAAAT 5341 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC 5401 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT 5461 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG 5521 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG 5581 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC 5641 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT 5701 GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT 5761 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG 5821 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG 5881 GATCTCCCTT TGGGCCGCCT CCCCGCggta cctttaagac caatgactta caaggcagct 5941 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 6001 cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 6061 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 6121 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 6181 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 6241 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 6301 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 6361 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 6421 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 6481 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 6541 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat 6601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 6661 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 6721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 6781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 7021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 7081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 7141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 7201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 7261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 7321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 7381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 7441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 7501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 7561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 7621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 7681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 7741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 7801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 7861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 7921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 8041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 8101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 8161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 8221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 8281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 8341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 8401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 8461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 8521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 8581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 8641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 8701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 8761 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 8821 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 8881 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 8941 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 9001 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt 9061 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 9121 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 9181 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.