pLV3-CMV-ELK3-3×FLAG-EF1a-CopG

pLV3-CMV-ELK3-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94716

pLV3-CMV-ELK3-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-ELK3(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_005230.4;ERP; NET; SAP-2; SAP2
Gene lenght: 1221bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ELK3-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-ELK3-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ELK3-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggagag tgcaatcacg ctgtggcagt tcctgttgca 2341 gttgctgctg gatcagaaac atgagcattt gatctgctgg acctcgaacg atggtgaatt 2401 caagctcctc aaagcagaag aagtggccaa gctgtgggga ctccgaaaaa acaaaacaaa 2461 tatgaactat gataagctga gcagagccct gcgatactat tatgacaaga acatcatcaa 2521 gaaggtgatc gggcagaagt ttgtgtacaa gtttgtctct ttcccggaga tcctgaagat 2581 ggatcctcac gcggtggaga tcagccggga gagccttctg ctgcaggaca gcgactgcaa 2641 ggcgtctccg gagggccgcg aggcccacaa acacggcctg gccgccctca gaagcacgag 2701 ccgcaacgaa tacatccact caggcctgta ctcgtccttc accattaatt ccctgcagaa 2761 cccaccagac gccttcaagg ccatcaagac ggagaagctg gaggagccgc ccgaagacag 2821 cccccccgtg gaagaagtca ggactgtgat caggtttgtg accaataaaa ccgacaagca 2881 cgtcaccagg ccggtggtgt ccctgccttc cacgtcagag gctgcggcgg cgtccgcctt 2941 cctggcctcg tccgtctcgg ccaagatctc ctctttaatg ttgccaaacg ctgccagtat 3001 ttcatccgcc tcacccttct catctcggtc cccgtccctg tcccccaact cacccctccc 3061 ttctgaacac agaagcctct tcctggaggc cgcctgccat gactccgatt ccctggagcc 3121 cttgaacctg tcatcgggct ccaagaccaa gtctccatct cttcccccaa aggccaaaaa 3181 acccaaaggc ttggaaatct cagcgccccc gctggtgctc tccggcaccg acatcggctc 3241 catcgccctc aacagcccag ccctcccctc gggatccctc accccagcct tcttcaccgc 3301 acagacacca aatggattgc ttctgactcc gagtccactg ctctccagca tacatttctg 3361 gagcagcctt agtccagttg ctccgctgag tcctgccagg ctgcaagggc caagcacgct 3421 gttccagttc cccacactgc ttaatggcca catgccagtg ccaatcccca gtctggacag 3481 agctgcttct ccagtactgc tttcttcaaa ctctcagaaa tccggatccG ACTACAAAGA 3541 CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAgcg 3601 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 3661 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 3721 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 3781 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 3841 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 3901 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 3961 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 4021 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 4081 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 4141 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 4201 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 4261 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 4321 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 4381 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 4441 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 4501 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 4561 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 4621 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 4681 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 4741 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 4801 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 4861 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 4921 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctGCCAC 4981 Catggctgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 5041 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 5101 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 5161 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 5221 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 5281 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 5341 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 5401 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 5461 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 5521 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 5581 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 5641 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 5701 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 5761 tgacgagttc ttctgaGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 5821 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 5881 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 5941 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 6001 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 6061 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 6121 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC 6181 ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 6241 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 6301 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 6361 CGCCTCCCCG Cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 6421 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG ataagatctg 6481 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 6541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 6601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 6661 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 6721 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 6781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 6841 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 6901 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 6961 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 7021 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 7081 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7141 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7201 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7261 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 7861 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 7921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 7981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 8041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 8821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 8881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 9001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 9061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9181 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 9241 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 9301 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 9361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 9421 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 9481 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 9541 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 9601 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 9661 gtaaaacgac ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.