pMSCV-miR30a-Nnt-shRNA-EGFP pl

pMSCV-miR30a-Nnt-shRNA-EGFP plasmid

PVT94567

pMSCV-miR30a-Nnt-shRNA-EGFP plasmid Information

Plasmid name: pMSCV-miR30a-Nnt(mouse)-shRNA-EGFP
Alias:
Gene lenght:
Host: Mammalian cells,Retrovirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter:
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PGK-R
Copy number: High
Induction:

pMSCV-miR30a-Nnt-shRNA-EGFP plasmid Description

pMSCV-miR30a-Nnt-shRNA-EGFP is a plasmid suitable for use in mammalian cells, and can also be used for retrovirus packaging.

pMSCV-miR30a-Nnt-shRNA-EGFP plasmid Sequence

1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1381 ccctcactcc ttctctaggc gccggaatta gatctctcga CTAGGGATAA CAGGGTAATT 1441 GTTTGAATGA GGCTTCAGTA CTTTACAGAA TCGTTGCCTG CACATCTTGG AAACACTTGC 1501 TGGGATTACT TCTTCAGGTT AACCCAACAG AAGGCTCGAG AAGGTATATT GCTGTTGACA 1561 GTGAGCGAcg ggagaacttt acgttcataT AGTGAAGCCA CAGATGTAta tgaacgtaaa 1621 gttctcccgG TGCCTACTGC CTCGGAATTC AAGGGGCTAC TTTAGGAGCA ATTATCTTGT 1681 TTACTAAAAC TGAATACCTT GCTATCTCTT TGATACATTT TTACAAAGCT GAATTAAAAT 1741 GGTATAAATT AAATCACTTT TTTCaattct accgggtagg ggaggcgctt ttcccaaggc 1801 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 1861 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 1921 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 1981 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca 2041 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 2101 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 2161 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 2221 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca 2281 gcccaagctt atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 2341 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 2401 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 2461 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 2521 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 2581 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 2641 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 2701 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 2761 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 2821 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 2881 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 2941 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 3001 gtacaagtag atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga 3061 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga 3121 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa 3181 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca 3241 gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca 3301 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct 3361 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc 3421 actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac 3481 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt 3541 gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca 3601 acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga 3661 atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa Agagctgggg 3721 agaattgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 3781 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 3841 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 3901 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 3961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 4021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 4081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 4141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 4201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 4261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 4321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 4381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 4441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 4501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 4561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 4621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 4681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 4741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 4801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 4861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 4921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 4981 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 5041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 5101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 5161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 5221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 5281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 5401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5461 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 5521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 5761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 5881 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 5941 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 6001 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 6061 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 6121 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 6181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 6241 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 6301 acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt 6361 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag 6421 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct 6481 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt 6541 aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag 6601 cctatagagt acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg 6661 ggaa

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.