pLV3-CMV-NEDD4-3×HA-CopGFP-Pur

pLV3-CMV-NEDD4-3×HA-CopGFP-Puro plasmid

PVT90416

pLV3-CMV-NEDD4-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NEDD4(human)-3×HA-CopGFP-Puro
Alias: NM_001284338.2;NEDD4-1; RPF1
Gene lenght: 3957bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NEDD4-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-NEDD4-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NEDD4-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cacaaagctt acgattgcac 2341 tttgcagcca gaagaagcaa tacttaccct ttgtcagaaa cctccggaga tgacttggat 2401 agccatgttc acatgtgctt caaaagacca acacggattt caacgtctaa cgttgttcaa 2461 atgaagctga ctcccagaca gactgcacta gctccgttaa taaaggaaaa cgttcagtct 2521 caagaaagat catctgttcc ctcatctgaa aatgttaata aaaagagcag ctgtctacag 2581 atttcactac agccaacaag gtacagtgga tatcttcagt ctagcaatgt cttagctgat 2641 agtgatgatg cttcgtttac ttgtatcttg aaggatggta tttacagtag tgctgtggtc 2701 gataatgaat tgaatgctgt gaatgatggt caccttgtaa gcagtccagc catttgtagt 2761 ggtagcctta gtaacttttc aaccagtgat aatgggtctt acagcagcaa cggtagtgat 2821 tttgggtcat gtgcaagtat cacaagtgga ggttcatata ctaacagtgt catcagtgac 2881 agtagtagtt atacttttcc accaagtgat gatacttttt tgggtggaaa cttaccttct 2941 gacagcacct ccaatagaag tgtgccaaac aggaatacta ctccttgtga aattttttca 3001 agaagtacaa gtacagatcc ttttgtccag gatgacttgg aacatggatt agagattatg 3061 aaattgccag tgagcaggaa cacaaaaatt ccactaaaac gttactcctc cttagtcatt 3121 tttcctagga gtccttcaac tacccgaccg acttctccaa caagtctgtg tactcttctg 3181 agcaaaggat cctatcaaac ttcacaccag tttattattt ctcctagtga aattgcacat 3241 aatgaggatg gcactagtgc taaaggattt ctttcaacag ctgtcaatgg acttcggtta 3301 tctaaaacaa tttgtacccc cggagaagta agagacatac ggccgcttca caggaagggc 3361 tcgttacaga agaaaattgt tctttcgaat aatactccca gacagactgt ctgtgaaaag 3421 tcatctgaag gatattcttg tgtttcagtg catttcaccc aacgaaaagc agctacatta 3481 gactgtgaaa caacaaatgg tgattgtaaa ccagaaatgt cagaaattaa gcttaattct 3541 gattcagagt atattaagct catgcatagg acatctgcat gtttgccatc ctcccaaaat 3601 gtagattgtc aaataaatat caatggagaa ttggaaagac cacattcaca gatgaacaaa 3661 aaccatggta ttttacgaag aagtatttca ttgggaggag cttatccaaa tatttcctgt 3721 ctatccagcc ttaagcacaa ttgttctaaa gggggaccat ctcagttact cataaagttt 3781 gcatctggaa atgaaggtaa agtggataat ttatcaagag acagcaacag agattgcaca 3841 aatgaactgt ctaattcttg caagacaaga gatgatttcc taggtcaagt ggatgttcca 3901 ctttatccat taccgacaga aaatccaaga ttggagagac catatacatt taaggatttt 3961 gttcttcatc caagaagtca caaatcaaga gttaaaggtt atctgagact aaaaatgact 4021 tatttaccta aaaccagtgg ctcagaagat gataatgcag aacaggctga ggaattagag 4081 cctggctggg ttgttttgga ccaaccagat gctgcttgcc atttgcagca acaacaagaa 4141 ccttctcctc tacctccagg gtgggaagag aggcaggata tccttggaag gacctattat 4201 gtaaaccatg aatctagaag aacacagtgg aaaagaccaa cccctcagga caacctaaca 4261 gatgctgaga atggcaacat tcaactgcaa gcacaacgtg catttaccac caggcggcag 4321 atatccgagg aaacagaaag tgttgacaac cgagagtctt ccgagaactg ggaaattata 4381 agagaagatg aagccaccat gtatagcaac caggccttcc catcacctcc accgtcaagt 4441 aacttggatg ttccaactca tcttgcagaa gaattgaatg ccagactcac catttttgga 4501 aattcagccg tgagccagcc agcatcgagc tcaaatcatt ccagcagaag aggcagctta 4561 caagcctata cttttgagga acaacctaca cttcctgtgc ttttgcctac ttcatctgga 4621 ttaccaccag gttgggaaga aaaacaagat gaaagaggaa gatcatatta tgtagatcac 4681 aattccagaa cgactacttg gacaaagccc actgtacagg ccacagtgga gaccagtcag 4741 ctgacctcaa gccagagttc tgcaggccct caatcacaag cctccaccag tgattcaggc 4801 cagcaggtga cccagccatc tgaaattgag caaggattcc ttcctaaagg ctgggaagtc 4861 cggcatgcac caaatgggag gcctttcttt attgaccaca acactaaaac caccacctgg 4921 gaagatccaa gattgaaaat tccagcccat ctgagaggaa agacatcact tgatacttcc 4981 aatgatctag ggcctttacc tccaggatgg gaagagagaa ctcacacaga tggaagaatc 5041 ttctacataa atcacaatat aaaaagaaca caatgggaag atcctcggtt ggagaatgta 5101 gcaataactg gaccagcagt gccctactcc agggattaca aaagaaagta tgagttcttc 5161 cgaagaaagt tgaagaagca gaatgacatt ccaaacaaat ttgaaatgaa acttcgccga 5221 gcaactgttc ttgaagactc ttaccggaga attatgggtg tcaagagagc agacttcctg 5281 aaggctcgac tgtggattga gtttgatggt gaaaagggat tggattatgg aggagttgcc 5341 agagaatggt tcttcctgat ctcaaaggaa atgtttaacc cttattatgg gttgtttgaa 5401 tattctgcta cggacaatta taccctacag ataaatccaa actctggatt gtgtaacgaa 5461 gatcacctct cttacttcaa gtttattggt cgggtagctg gaatggcagt ttatcatggc 5521 aaactgttgg atggtttttt catccgccca ttttacaaga tgatgcttca caaaccaata 5581 acccttcatg atatggaatc tgtggatagt gaatattaca attccctaag atggattctt 5641 gaaaatgacc caacagaatt ggacctcagg tttatcatag atgaagaact ttttggacag 5701 acacatcaac atgagctgaa aaatggtgga tcagaaatag ttgtcaccaa taagaacaaa 5761 aaggaatata tttatcttgt aatacaatgg cgatttgtaa accgaatcca gaagcaaatg 5821 gctgctttta aagagggatt ctttgaacta ataccacagg atctcatcaa aatttttgat 5881 gaaaatgaac tagagcttct tatgtgtgga ctgggagatg ttgatgtgaa tgactggagg 5941 gaacatacaa agtataaaaa tggctacagt gcaaatcatc aggttataca gtggttttgg 6001 aaggctgttt taatgatgga ttcagaaaaa agaataagat tacttcagtt tgtcactggc 6061 acatctcggg tgcctatgaa tggatttgct gaactatacg gttcaaatgg accacagtca 6121 tttacagttg aacagtgggg tactcctgaa aagctgccaa gagctcatac ctgttttaat 6181 cgcctggact tgccacctta tgaatcattt gaagaattat gggataaact tcagatggca 6241 attgaaaaca cccagggctt tgatggagtt gatCTCGAGT ACCCATACGA CGTACCTGAT 6301 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 6361 TACGCATAGg gatccCTGCG ATATCCAGCg cggccgcGaa ggatctgcga tcgctccggt 6421 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 6481 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 6541 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 6601 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 6661 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 6721 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 6781 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 6841 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 6901 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 6961 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 7021 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 7081 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 7141 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 7201 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 7261 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 7321 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 7381 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 7441 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 7501 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 7561 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 7621 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 7681 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 7741 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 7801 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 7861 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 7921 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 7981 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 8041 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 8101 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 8161 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 8221 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 8281 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 8341 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 8401 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 8461 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 8521 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 8581 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 8641 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 8701 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 8761 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 8821 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 8881 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt 8941 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 9001 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 9061 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 9121 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 9181 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 9241 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 9301 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 9361 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 9421 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 9481 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 9541 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 9601 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 9661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 9721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 9781 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 9841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 9901 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 9961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 10021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 10081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 10141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 10201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 10261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 10321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 10381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 10441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 10501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 10561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 10621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 10681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 10741 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 10801 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 10861 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 10921 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 10981 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 11041 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 11101 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 11161 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 11221 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 11281 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 11341 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 11401 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 11461 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 11521 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 11581 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 11641 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 11701 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 11761 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 11821 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 11881 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 11941 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 12001 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 12061 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 12121 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 12181 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 12241 gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.