pCMV-GST-3×FLAG-RPS6KA1-Neo pl

pCMV-GST-3×FLAG-RPS6KA1-Neo plasmid

PVT94530

pCMV-GST-3×FLAG-RPS6KA1-Neo plasmid Information

Plasmid name: pCMV-GST-3×FLAG-RPS6KA1(human)-Neo
Alias: NM_002953.4;HU-1; MAPKAPK1; MAPKAPK1A; p90Rsk; RSK;RSK1
Gene lenght: 2208bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: High
Induction:

pCMV-GST-3×FLAG-RPS6KA1-Neo plasmid Description

pCMV-GST-3×FLAG-RPS6KA1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GST-3×FLAG-RPS6KA1-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCcacca 721 tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt 781 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat 841 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg 901 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca 961 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg 1021 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg 1081 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa 1141 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg 1201 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa 1261 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat 1321 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaaGGAGGAG 1381 GGGGAAGCgG CGGCGGAGGG AGTaagcttg actacaaaga ccatgacggt gattataaag 1441 atcatgacat cgactacaag gatgacgatg acaagcttAT GCCGCTCGCC CAGCTCAAGG 1501 AGCCCTGGCC GCTCATGGAG CTAGTGCCTC TGGACCCGGA GAATGGACAG ACCTCAGGGG 1561 AAGAAGCTGG ACTTCAGCCG TCCAAGGATG AGGGCGTCCT CAAGGAGATC TCCATCACGC 1621 ACCACGTCAA GGCTGGCTCT GAGAAGGCTG ATCCATCCCA TTTCGAGCTC CTCAAGGTTC 1681 TGGGCCAGGG ATCCTTTGGC AAAGTCTTCC TGGTGCGGAA AGTCACCCGG CCTGACAGTG 1741 GGCACCTGTA TGCTATGAAG GTGCTGAAGA AGGCAACGCT GAAAGTACGT GACCGCGTCC 1801 GGACCAAGAT GGAGAGAGAC ATCCTGGCTG ATGTAAATCA CCCATTCGTG GTGAAGCTGC 1861 ACTATGCCTT CCAGACCGAG GGCAAGCTCT ATCTCATTCT GGACTTCCTG CGTGGTGGGG 1921 ACCTCTTCAC CCGGCTCTCA AAAGAGGTGA TGTTCACGGA GGAGGATGTG AAGTTTTACC 1981 TGGCTGAGCT GGCTCTGGGC CTGGATCACC TGCACAGCCT GGGTATCATT TACAGAGACC 2041 TCAAGCCTGA GAACATCCTT CTGGATGAGG AGGGCCACAT CAAACTCACT GACTTTGGCC 2101 TGAGCAAAGA GGCCATTGAC CACGAGAAGA AGGCCTATTC TTTCTGCGGG ACAGTGGAGT 2161 ACATGGCCCC TGAGGTCGTC AACCGCCAGG GCCACTCCCA TAGTGCGGAC TGGTGGTCCT 2221 ATGGGGTGTT GATGTTTGAG ATGCTGACGG GCTCCCTGCC CTTCCAGGGG AAGGACCGGA 2281 AGGAGACCAT GACACTGATT CTGAAGGCGA AGCTAGGCAT GCCCCAGTTT CTGAGCACTG 2341 AAGCCCAGAG CCTCTTGCGG GCCCTGTTCA AGCGGAATCC TGCCAACCGG CTCGGCTCCG 2401 GCCCTGATGG GGCAGAGGAA ATCAAGCGGC ATGTCTTCTA CTCCACCATT GACTGGAATA 2461 AGCTATACCG TCGTGAGATC AAGCCACCCT TCAAGCCAGC AGTGGCTCAG CCTGATGACA 2521 CCTTCTACTT TGACACCGAG TTCACGTCCC GCACACCCAA GGATTCCCCA GGCATCCCCC 2581 CCAGCGCTGG GGCCCATCAG CTGTTCCGGG GCTTCAGCTT CGTGGCCACC GGCCTGATGG 2641 AAGACGACGG CAAGCCTCGT GCCCCGCAGG CACCCCTGCA CTCGGTGGTA CAGCAACTCC 2701 ATGGGAAGAA CCTGGTTTTT AGTGACGGCT ACGTGGTAAA GGAGACAATT GGTGTGGGCT 2761 CCTACTCTGA GTGCAAGCGC TGTGTCCACA AGGCCACCAA CATGGAGTAT GCTGTCAAGG 2821 TCATTGATAA GAGCAAGCGG GATCCTTCAG AAGAGATTGA GATTCTTCTG CGGTATGGCC 2881 AGCACCCCAA CATCATCACT CTGAAAGATG TGTATGATGA TGGCAAACAC GTGTACCTGG 2941 TGACAGAGCT GATGCGGGGT GGGGAGCTGC TGGACAAGAT CCTGCGGCAG AAGTTCTTCT 3001 CAGAGCGGGA GGCCAGCTTT GTCCTGCACA CCATTGGCAA AACTGTGGAG TATCTGCACT 3061 CACAGGGGGT TGTGCACAGG GACCTGAAGC CCAGCAACAT CCTGTATGTG GACGAGTCCG 3121 GGAATCCCGA GTGCCTGCGC ATCTGTGACT TTGGTTTTGC CAAACAGCTG CGGGCTGAGA 3181 ATGGGCTCCT CATGACACCT TGCTACACAG CCAACTTTGT GGCGCCTGAG GTGCTGAAGC 3241 GCCAGGGCTA CGATGAAGGC TGCGACATCT GGAGCCTGGG CATTCTGCTG TACACCATGC 3301 TGGCAGGATA TACTCCATTT GCCAACGGTC CCAGTGACAC ACCAGAGGAA ATCCTAACCC 3361 GGATCGGCAG TGGGAAGTTT ACCCTCAGTG GGGGAAATTG GAACACAGTT TCAGAGACAG 3421 CCAAGGACCT GGTGTCCAAG ATGCTACACG TGGATCCCCA CCAGCGCCTC ACAGCTAAGC 3481 AGGTTCTGCA GCATCCATGG GTCACCCAGA AAGACAAGCT TCCCCAAAGC CAGCTGTCCC 3541 ACCAGGACCT ACAGCTTGTG AAGGGAGCCA TGGCTGCCAC GTACTCCGCA CTCAACAGCT 3601 CCAAGCCCAC CCCCCAGCTG AAGCCCATCG AGTCATCCAT CCTGGCCCAG CGGCGAGTGA 3661 GGAAGTTGCC ATCCACCACC CTGTGAgaat tctgcagata tccagcacag tggcggccgc 3721 tcgagtctag agggcccGAT ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 3781 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 3841 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 3901 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 3961 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 4021 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 4081 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 4141 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 4201 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 4261 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 4321 TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA 4381 CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG 4441 GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC 4501 GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG 4561 TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA 4621 TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 4681 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 4741 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 4801 AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 4861 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 4921 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 4981 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG 5041 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 5101 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5161 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 5221 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 5281 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 5341 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 5401 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 5461 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 5521 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 5581 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 5641 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 5701 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 5761 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 5821 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 5881 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 5941 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 6001 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 6061 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 6121 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 6181 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 6241 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 6301 TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 6361 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 6421 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 6481 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 6541 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 6601 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 6661 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 6721 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 6781 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 6841 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 6901 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 6961 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 7021 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 7081 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 7141 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 7201 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 7261 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 7321 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 7381 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 7441 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 7501 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 7561 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 7621 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 7681 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 7741 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 7801 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 7861 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 7921 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 7981 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 8041 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 8101 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 8161 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 8221 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 8281 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 8341 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 8401 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 8461 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 8521 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 8581 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 8641 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 8701 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 8761 ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG 8821 TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT 8881 TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC 8941 TTGACCGACA ATTGCATGAA GAATCTGCTT AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.