pCMV-PML-3×HA-Neo plasmid

pCMV-PML-3×HA-Neo plasmid

PVT97075

pCMV-PML-3×HA-Neo plasmid Information

Plasmid name: pCMV-PML(human)-3×HA-Neo
Alias: NM_033238.3;MYL; PP8675; RNF71; TRIM19
Gene lenght: 2646bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PML-3×HA-Neo plasmid Description

pCMV-PML-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PML-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCgcca ccatggagcc tgcacccgcc cgatctccga ggccccagca 721 ggaccccgcc cggccccagg agcccaccat gcctcccccc gagaccccct ctgaaggccg 781 ccagcccagc cccagcccca gccctacaga gcgagccccc gcttcggagg aggagttcca 841 gtttctgcgc tgccagcaat gccaggcgga agccaagtgc ccgaagctgc tgccttgtct 901 gcacacgctg tgctcaggat gcctggaggc gtcgggcatg cagtgcccca tctgccaggc 961 gccctggccc ctaggtgcag acacacccgc cctggataac gtctttttcg agagtctgca 1021 gcggcgcctg tcggtgtacc ggcagattgt ggatgcgcag gctgtgtgca cccgctgcaa 1081 agagtcggcc gacttctggt gctttgagtg cgagcagctc ctctgcgcca agtgcttcga 1141 ggcacaccag tggttcctca agcacgaggc ccggccccta gcagagctgc gcaaccagtc 1201 ggtgcgtgag ttcctggacg gcacccgcaa gaccaacaac atcttctgct ccaaccccaa 1261 ccaccgcacc cctacgctga ccagcatcta ctgccgagga tgttccaagc cgctgtgctg 1321 ctcgtgcgcg ctccttgaca gcagccacag tgagctcaag tgcgacatca gcgcagagat 1381 ccagcagcga caggaggagc tggacgccat gacgcaggcg ctgcaggagc aggatagtgc 1441 ctttggcgcg gttcacgcgc agatgcacgc ggccgtcggc cagctgggcc gcgcgcgtgc 1501 cgagaccgag gagctgatcc gcgagcgcgt gcgccaggtg gtagctcacg tgcgggctca 1561 ggagcgcgag ctgctggagg ctgtggacgc gcggtaccag cgcgactacg aggagatggc 1621 cagtcggctg ggccgcctgg atgctgtgct gcagcgcatc cgcacgggca gcgcgctggt 1681 gcagaggatg aagtgctacg cctcggacca ggaggtgctg gacatgcacg gtttcctgcg 1741 ccaggcgctc tgccgcctgc gccaggagga gccccagagc ctgcaagctg ccgtgcgcac 1801 cgatggcttc gacgagttca aggtgcgcct gcaggacctc agctcttgca tcacccaggg 1861 gaaagatgca gctgtatcca agaaagccag cccagaggct gccagcactc ccagggaccc 1921 tattgacgtt gacctgcccg aggaggcaga gagagtgaag gcccaggttc aggccctggg 1981 gctggctgaa gcccagccta tggctgtggt acagtcagtg cccggggcac accccgtgcc 2041 agtgtacgcc ttctccatca aaggcccttc ctatggagag gatgtctcca atacaacgac 2101 agcccagaag aggaagtgca gccagaccca gtgccccagg aaggtcatca agatggagtc 2161 tgaggagggg aaggaggcaa ggttggctcg gagctccccg gagcagccca ggcccagcac 2221 ctccaaggca gtctcaccac cccacctgga tggaccgcct agccccagga gccccgtcat 2281 aggaagtgag gtcttcctgc ccaacagcaa ccacgtggcc agtggcgccg gggaggcaga 2341 ggaacgcgtt gtggtgatca gcagctcgga agactcagat gccgaaaact cgtcctcccg 2401 agagctggat gacagcagca gtgagtccag tgacctccag ctggaaggcc ccagcaccct 2461 cagggtcctg gacgagaacc ttgctgaccc ccaagcagaa gacagacctc tggttttctt 2521 tgacctcaag attgacaatg aaacccagaa gattagccag ctggctgcgg tgaaccggga 2581 aagcaagttc cgcgtggtca tccagcctga agccttcttc agcatctact ccaaggccgt 2641 gtccctggag gtggggctgc agcacttcct cagctttctg agctccatgc gccgccctat 2701 cttggcctgc tacaagctgt gggggcctgg cctcccaaac ttcttccggg ccctggagga 2761 cattaacagg ctgtgggaat tccaggaggc catctcgggc ttcctggctg ccctgcctct 2821 catccgggag cgtgtgcccg gggccagcag cttcaaactc aagaacctgg cccagaccta 2881 cctggcgaga aacatgagcg agcgcagcgc catggctgcc gtgctggcca tgcgtgacct 2941 gtgccgcctc ctcgaggtct ccccgggccc ccagctggcc cagcatgtct accccttcag 3001 tagcctgcag tgctttgcct ccctgcagcc cctggtgcag gcagctgtgc tgccccgggc 3061 tgaggcccgc ctcctggccc tacacaacgt gagcttcatg gagctgctga gtgcacaccg 3121 ccgtgaccgg caggggggcc tgaagaagta cagccgctat ctaagcctgc agaccaccac 3181 gttgccccct gcccagcctg ctttcaacct gcaggctctg ggcacctact ttgaaggcct 3241 gttggagggt ccggcgctgg cacgggcaga aggagtctcc accccacttg ctggccgtgg 3301 cttggcagag agggcctccc agcagagcGA ATTCTGCCTC GAGTACCCAT ACGACGTACC 3361 TGATTATGCA ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC 3421 AGACTACGCA TAGGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 3481 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 3541 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 3601 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 3661 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 3721 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 3781 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG 3841 CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 3901 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 3961 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 4021 GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG 4081 AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG 4141 TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA 4201 CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG 4261 GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG 4321 CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 4381 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 4441 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 4501 CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 4561 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 4621 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 4681 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA 4741 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT 4801 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 4861 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 4921 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 4981 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 5041 CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 5101 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 5161 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC 5221 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 5281 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 5341 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 5401 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 5461 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 5521 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 5581 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 5641 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 5701 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 5761 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 5821 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC 5881 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 5941 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC 6001 GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC 6061 TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG 6121 CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT 6181 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT 6241 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC 6301 TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 6361 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 6421 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 6481 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 6541 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 6601 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 6661 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 6721 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 6781 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 6841 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 6901 TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 6961 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 7021 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 7081 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 7141 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 7201 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 7261 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 7321 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 7381 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 7441 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 7501 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 7561 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 7621 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 7681 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 7741 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 7801 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 7861 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 7921 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 7981 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 8041 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 8101 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 8161 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 8221 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 8281 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 8341 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 8401 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 8461 CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA 8521 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG 8581 TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA 8641 CCGACAATTG CATGAAGAAT CTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.