pCMV-3×HA-CNR1-Neo plasmid

pCMV-3×HA-CNR1-Neo plasmid

PVT94240

pCMV-3×HA-CNR1-Neo plasmid Information

Plasmid name: pCMV-3×HA-CNR1(human)-Neo
Alias: NM_016083.6;CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR
Gene lenght: 1419bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-CNR1-Neo plasmid Description

pCMV-3×HA-CNR1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-CNR1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGTAC CCATACGACG TACCTGATTA TGCAACTTAT 961 CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATCAGCG 1021 GAAAAGCTTa tgaagtcgat cctagatggc cttgcagata ccaccttccg caccatcacc 1081 actgacctcc tgtacgtggg ctcaaatgac attcagtacg aagacatcaa aggtgacatg 1141 gcatccaaat tagggtactt cccacagaaa ttccctttaa cttcctttag gggaagtccc 1201 ttccaagaga agatgactgc gggagacaac ccccagctag tcccagcaga ccaggtgaac 1261 attacagaat tttacaacaa gtctctctcg tccttcaagg agaatgagga gaacatccag 1321 tgtggggaga acttcatgga catagagtgt ttcatggtcc tgaaccccag ccagcagctg 1381 gccattgcag tcctgtccct cacgctgggc accttcacgg tcctggagaa cctcctggtg 1441 ctgtgcgtca tcctccactc ccgcagcctc cgctgcaggc cttcctacca cttcatcggc 1501 agcctggcgg tggcagacct cctggggagt gtcatttttg tctacagctt cattgacttc 1561 cacgtgttcc accgcaaaga tagccgcaac gtgtttctgt tcaaactggg tggggtcacg 1621 gcctccttca ctgcctccgt gggcagcctg ttcctcacag ccatcgacag gtacatatcc 1681 attcacaggc ccctggccta taagaggatt gtcaccaggc ccaaggccgt ggtggcgttt 1741 tgcctgatgt ggaccatagc cattgtgatc gccgtgctgc ctctcctggg ctggaactgc 1801 gagaaactgc aatctgtttg ctcagacatt ttcccacaca ttgatgaaac ctacctgatg 1861 ttctggatcg gggtcaccag cgtactgctt ctgttcatcg tgtatgcgta catgtatatt 1921 ctctggaagg ctcacagcca cgccgtccgc atgattcagc gtggcaccca gaagagcatc 1981 atcatccaca cgtctgagga tgggaaggta caggtgaccc ggccagacca agcccgcatg 2041 gacattaggt tagccaagac cctggtcctg atcctggtgg tgttgatcat ctgctggggc 2101 cctctgcttg caatcatggt gtatgatgtc tttgggaaga tgaacaagct cattaagacg 2161 gtgtttgcat tctgcagtat gctctgcctg ctgaactcca ccgtgaaccc catcatctat 2221 gctctgagga gtaaggacct gcgacacgct ttccggagca tgtttccctc ttgtgaaggc 2281 actgcgcagc ctctggataa cagcatgggg gactcggact gcctgcacaa acacgcaaac 2341 aatgcagcca gtgttcacag ggccgcagaa agctgcatca agagcacggt caagattgcc 2401 aaggtaacca tgtctgtgtc cacagacacg tctgccgagg ctctgtgaga attctgcaga 2461 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc 2521 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 2581 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 2641 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 2701 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 2761 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 2821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 2881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 3001 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 3061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 3121 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 3181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 3241 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3301 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 3361 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 3421 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 3481 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 3541 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 3601 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 3661 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 3721 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 3781 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 3841 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 3901 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 3961 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 4021 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 4081 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 4141 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 4201 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 4261 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 4321 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 4381 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 4441 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 4501 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 4561 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 4621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 4681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 4741 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 4801 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 4861 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 4921 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4981 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 5041 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 5101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 5161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 5401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5581 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 5641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 5701 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5761 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5821 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5881 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5941 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6001 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6061 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6121 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6181 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6241 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6301 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6361 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6421 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6481 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6541 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6601 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6661 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6721 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6781 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6841 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6901 tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.