pLV3-CMV-FZD8-3×FLAG-CopGFP-Pu

pLV3-CMV-FZD8-3×FLAG-CopGFP-Puro plasmid

PVT94150

pLV3-CMV-FZD8-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FZD8(human)-3×FLAG-CopGFP-Puro
Alias: NM_031866.3;FZ-8; hFZ8
Gene lenght: 2082bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FZD8-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FZD8-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FZD8-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agtggggtta cctgttggaa 2341 gtgacctcgc tgctggccgc cttggcgctg ctgcagcgct ctagcggcgc tgcggccgcc 2401 tcggccaagg agctggcatg ccaagagatc accgtgccgc tgtgtaaggg catcggctac 2461 aactacacct acatgcccaa tcagttcaac cacgacacgc aagacgaggc gggcctggag 2521 gtgcaccagt tctggccgct ggtggagatc cagtgctcgc ccgatctcaa gttcttcctg 2581 tgcagcatgt acacgcccat ctgcctagag gactacaaga agccgctgcc gccctgccgc 2641 tcggtgtgcg agcgcgccaa ggccggctgc gcgccgctca tgcgccagta cggcttcgcc 2701 tggcccgacc gcatgcgctg cgaccggctg cccgagcaag gcaaccctga cacgctgtgc 2761 atggactaca accgcaccga cctaaccacc gccgcgccca gcccgccgcg ccgcctgccg 2821 ccgccgccgc ccggcgagca gccgccttcg ggcagcggcc acggccgccc gccgggggcc 2881 aggcccccgc accgcggcgg cggcaggggc ggtggcggcg gggacgcggc ggcgccccca 2941 gctcgcggcg gcggcggtgg cgggaaggcg cggccccctg gcggcggcgc ggctccctgc 3001 gagcccgggt gccagtgccg cgcgcctatg gtgagcgtgt ccagcgagcg ccacccgctc 3061 tacaaccgcg tcaagacagg ccagatcgct aactgcgcgc tgccctgcca caaccccttt 3121 ttcagccagg acgagcgcgc cttcaccgtc ttctggatcg gcctgtggtc ggtgctctgc 3181 ttcgtgtcca ccttcgccac cgtctccacc ttccttatcg acatggagcg cttcaagtac 3241 ccggagcggc ccattatctt cctctcggcc tgctacctct tcgtgtcggt gggctaccta 3301 gtgcgcctgg tggcgggcca cgagaaggtg gcgtgcagcg gtggcgcgcc gggcgcgggg 3361 ggcgctgggg gcgcgggcgg cgcggcggcg ggcgcgggcg cggcgggcgc gggcgcgggc 3421 ggcccgggcg ggcgcggcga gtacgaggag ctgggcgcgg tggagcagca cgtgcgctac 3481 gagaccaccg gccccgcgct gtgcaccgtg gtcttcttgc tggtctactt cttcggcatg 3541 gccagctcca tctggtgggt gatcttgtcg ctcacatggt tcctggcggc cggtatgaag 3601 tggggcaacg aagccatcgc cggctactcg cagtacttcc acctggccgc gtggcttgtg 3661 cccagcgtca agtccatcgc ggtgctggcg ctcagctcgg tggacggcga cccggtggcg 3721 ggcatctgct acgtgggcaa ccagagcctg gacaacctgc gcggcttcgt gctggcgccg 3781 ctggtcatct acctcttcat cggcaccatg ttcctgctgg ccggcttcgt gtccctcttc 3841 cgcatccgct cggtcatcaa gcaacaggac ggccccacca agacgcacaa gctggagaag 3901 ctgatgatcc gcctgggcct gttcaccgtg ctctacaccg tgcccgccgc ggtggtggtc 3961 gcctgcctct tctacgagca gcacaaccgc ccgcgctggg aggccacgca caactgcccg 4021 tgcctgcggg acctgcagcc cgaccaggca cgcaggcccg actacgccgt cttcatgctc 4081 aagtacttca tgtgcctagt ggtgggcatc acctcgggcg tgtgggtctg gtccggcaag 4141 acgctggagt cctggcgctc cctgtgcacc cgctgctgct gggccagcaa gggcgccgcg 4201 gtgggcgggg gcgcgggcgc cacggccgcg gggggtggcg gcgggccggg gggcggcggc 4261 ggcgggggac ccggcggcgg cggggggccg ggcggcggcg ggggctccct ctacagcgac 4321 gtcagcactg gcctgacgtg gcggtcgggc acggcgagct ccgtgtctta tccaaagcag 4381 atgccattgt cccaggtcgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 4441 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 4501 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4561 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4621 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4681 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4741 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4801 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4861 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4921 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4981 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 5041 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 5101 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 5161 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 5221 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 5281 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 5341 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 5401 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 5461 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 5521 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 5581 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5641 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5701 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5761 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5821 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5881 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5941 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 6001 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 6061 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 6121 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 6181 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 6241 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 6301 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 6361 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 6421 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 6481 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6541 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6601 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6661 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6721 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6841 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6901 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6961 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 7021 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 7081 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 7141 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7201 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7261 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7321 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7381 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 7441 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 7501 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 7561 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 7621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7681 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7741 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7801 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7861 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7921 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8581 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 9001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 9241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 9361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 9421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 9481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 9541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9961 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 10021 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10081 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 10141 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 10201 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 10261 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 10321 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.