pCMV-3×FLAG-PLOD2-Neo plasmid

pCMV-3×FLAG-PLOD2-Neo plasmid

PVT94122

pCMV-3×FLAG-PLOD2-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-PLOD2(human)-Neo
Alias: NM_000935.3;BRKS2; LH2; TLH
Gene lenght: 2214bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-PLOD2-Neo plasmid Description

pCMV-3×FLAG-PLOD2-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-PLOD2-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggggggat gcacggtgaa gcctcagctg 1021 ctgctcctgg cgctcgtcct ccacccctgg aatccctgtc tgggtgcgga ctcggagaag 1081 ccctcgagca tccccacaga taaattatta gtcataactg tagcaacaaa agaaagtgat 1141 ggattccatc gatttatgca gtcagccaaa tatttcaatt atactgtgaa ggtccttggt 1201 caaggagaag aatggagagg tggtgatgga attaatagta ttggaggggg ccagaaagtg 1261 agattaatga aagaagtcat ggaacactat gctgatcaag atgatctggt tgtcatgttt 1321 actgaatgct ttgatgtcat atttgctggt ggtccagaag aagttctaaa aaaattccaa 1381 aaggcaaacc acaaagtggt ctttgcagca gatggaattt tgtggccaga taaaagacta 1441 gcagacaagt atcctgttgt gcacattggg aaacgctatc tgaattcagg aggatttatt 1501 ggctatgctc catatgtcaa ccgtatagtt caacaatgga atctccagga taatgatgat 1561 gatcagctct tttacactaa agtttacatt gatccactga aaagggaagc tattaacatc 1621 acattggatc acaaatgcaa aattttccag accttaaatg gagctgtaga tgaagttgtt 1681 ttaaaatttg aaaatggcaa agccagagct aagaatacat tttatgaaac attaccagtg 1741 gcaattaatg gaaatggacc caccaagatt ctcctgaatt attttggaaa ctatgtaccc 1801 aattcatgga cacaggataa tggctgcact ctttgtgaat tcgatacagt cgacttgtct 1861 gcagtagatg tccatccaaa cgtatcaata ggtgttttta ttgagcaacc aacccctttt 1921 ctacctcggt ttctggacat attgttgaca ctggattacc caaaagaagc acttaaactt 1981 tttattcata acaaagaagt ttatcatgaa aaggacatca aggtattttt tgataaagct 2041 aagcatgaaa tcaaaactat aaaaatagta ggaccagaag aaaatctaag tcaagcggaa 2101 gccagaaaca tgggaatgga cttttgccgt caggatgaaa agtgtgatta ttactttagt 2161 gtggatgcag atgttgtttt gacaaatcca aggactttaa aaattttgat tgaacaaaac 2221 agaaagatca ttgctcctct tgtaactcgt catggaaagc tgtggtccaa tttctgggga 2281 gcattgagtc ctgatggata ctatgcacga tctgaagatt atgtggatat tgttcaaggg 2341 aatagagtag gagtatggaa tgtcccatat atggctaatg tgtacttaat taaaggaaag 2401 acactccgat cagagatgaa tgaaaggaac tattttgttc gtgataaact ggatcctgat 2461 atggctcttt gccgaaatgc tagagaaatg ggtgtattta tgtacatttc taatagacat 2521 gaatttggaa ggctattatc cactgctaat tacaatactt cccattataa caatgacctc 2581 tggcagattt ttgaaaatcc tgtggactgg aaggaaaagt atataaaccg tgattattca 2641 aagattttca ctgaaaatat agttgaacag ccctgtccag atgtcttttg gttccccata 2701 ttttctgaaa aagcctgtga tgaattggta gaagaaatgg aacattacgg caaatggtct 2761 gggggaaaac atcatgatag ccgtatatct ggtggttatg aaaatgtccc aactgatgat 2821 atccacatga agcaagttga tctggagaat gtatggcttc attttatccg ggagttcatt 2881 gcaccagtta cactgaaggt ctttgcaggc tattatacga agggatttgc actactgaat 2941 tttgtagtaa aatactcccc tgaacgacag cgttctcttc gtcctcatca tgatgcttct 3001 acatttacca taaacattgc acttaataac gtgggagaag actttcaggg aggtggttgc 3061 aaatttctaa ggtacaattg ctctattgag tcaccacgaa aaggctggag cttcatgcat 3121 cctgggagac tcacacattt gcatgaagga cttcctgtta aaaatggaac aagatacatt 3181 gcagtgtcat ttatagatcc ctaagaattc tgcagatatc cagcacagtg gcggccgctc 3241 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 3301 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 3361 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 3421 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 3481 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 3541 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 3601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 3661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 3721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 3781 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 3841 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 3901 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 3961 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 4021 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 4081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4141 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 4201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4261 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4321 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat 4381 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 4441 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 4501 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 4561 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 4621 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 4681 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 4741 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 4801 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 4861 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca 4921 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 4981 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 5041 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 5101 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 5161 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 5221 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 5281 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 5341 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 5401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 5461 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 5521 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 5581 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 5641 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 5701 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 5761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 5821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 5881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 5941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6361 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa 6481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 6541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 6601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 6661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 6721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 6781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 6841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 6901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 6961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 7021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 7081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 7141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 7201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 7321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 7381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 7441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 7501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 7561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 7621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 7681 c

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.