pCMV-Copg1-3×HA-Neo plasmid

pCMV-Copg1-3×HA-Neo plasmid

PVT94047

pCMV-Copg1-3×HA-Neo plasmid Information

Plasmid name: pCMV-Copg1(mouse)-3×HA-Neo
Alias: NM_017477.2;Copg; D6Ertd71e; D6Wsu16e
Gene lenght: 2622bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Copg1-3×HA-Neo plasmid Description

pCMV-Copg1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Copg1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtt gaagaaattc 721 gacaagaagg acgaggagtc tggtggaggc tccaaccccc tccagcacct ggagaagagt 781 gccgtactcc aagaggctcg ggtctttaac gaaactccca tcaatccccg gaaatgtgct 841 cacatcctca ccaagatcct ttatctcata aaccaggggg agcacctggg gaccacggaa 901 gcaactgagg ctttctttgc catgaccaag ctcttccagt ccaatgatcc cacactccgc 961 cgcatgtgct atttgaccat caaggagatg tcctgcatcg ctgaggatgt catcattgtg 1021 acaagcagcc tgacaaaaga catgactggg aaagaggata attaccgcgg tcctgctgtc 1081 cgtgccctct gccagatcac tgacagcacc atgctgcagg ctgttgaacg ctacatgaaa 1141 caagcaattg tggacaaggt tcccagtgtc tccagctccg ccctcgtgtc ttccctgcac 1201 ctgctgaaat gcagcttcga tgtggtcaag cgctgggtga acgaggccca ggaggcggcg 1261 tccagtgaca acatcatggt ccagtaccat gccctaggac ttctgtacca tgtgcggaag 1321 aatgaccgac tggccgtgag taagatgatc agtaagttca cccggcacgg cctcaagtcc 1381 ccctttgcct actgcatgat gatccgagtg gccagcaagc aactagagga agaagacggc 1441 agccgtgaca gcccgctgtt tgacttcatc gagagctgcc tgcgtaacaa gcatgagatg 1501 gtggtgtatg aagctgcctc agccattgtc aacctgccag gctgcagtgc caaagagctg 1561 gcccctgctg tgtcagtgct ccagcttttc tgtagctcac ccaaggctgc acttcgctat 1621 gctgctgtgc gcaccctcaa taaggtggct atgaagcacc cttcagcggt gactgcctgc 1681 aatctagatc tggaaaacct ggttacagac tcaaacagga gcatcgccac attggccatc 1741 accaccctgc tcaagacagg gagcgagagc agcattgacc gccttatgaa gcagatttcc 1801 tccttcatgt cggagatctc agatgagttc aaggtggtgg ttgtccaggc catcagtgcc 1861 ctgtgccaga agtatcctcg aaagcatgct gtgctcatga acttcctgtt caccatgcta 1921 agggaagagg gtggctttga gtacaagcgt gccattgtgg actgcatcat cagcatcatc 1981 gaggagaact cagagagcaa ggagacagga ttgtcacacc tgtgcgagtt cattgaggac 2041 tgcgagttca ctgtgctggc cacccgcatc ctgcacctgt tgggccagga ggggcccaag 2101 accaacaacc cttccaagta cattcgcttt atctacaacc gtgtggtctt ggagcatgaa 2161 gaagttcggg caggtgcagt aagtgctctg gccaagtttg gagctcagaa cgaagagatg 2221 ttgcctagta tcttggtgtt gctgaagagg tgtgtgatgg acgacgacaa tgaagtgaga 2281 gacagagcca ccttctatct gaacgttctg gaacagaagc agaaggctct caacgcaggt 2341 tacatcctaa atggtctgac cgtgtccatc cctggtctgg agaaagccct gcagcagtac 2401 acactcgaac catcagagaa gccatttgac ctcaagtctg tgcctctggc caccacgcct 2461 atggcagagc agagaccaga aagcaccgcc accgcagcag tcaaacagcc ggagaaggta 2521 gcagccacac ggcaggagat tttccaagag cagctagcag cggtgcctga gttccaggga 2581 ctagggcctc tcttcaagtc ctctcctgag ccagtggccc tcacagagtc cgagaccgag 2641 tatgtcatcc gttgcaccaa acacaccttc tctgatcact tggtgttcca gtttgactgc 2701 acaaacaccc tcaatgacca gactctggag aatgtcacag tgcagatgga gcccactgag 2761 gcatacgaag tgctctcgta tgtgcctgcg cggagcttgc cctacaacca gcctgggacc 2821 tgctacacac tagtggctct gcccactgaa gaccccacag ctgtggcatg cacgttcagc 2881 tgtgtgatga agttcactgt taaggactgt gatcccaaca caggagaaat cgatgaagaa 2941 ggctatgagg atgagtatgt gctggaggat ctggaagtta ctgtggctga tcacatccaa 3001 aaagtcatga aagtgaactt tgaggcagcc tgggatgagg ttggggatga atttgagaag 3061 gaggaaacgt tcaccctgtc tactatcaag acactcgaag aggctgtggg caatattgtg 3121 aagttcctgg gaatgcatcc ttgtgagagg tcagacaaag tgccagaaaa caagaacacc 3181 cacacgctgc tgctagctgg agtattccgg ggtggtcatg acatccttgt gcgttctcgg 3241 cttctgcttt tggacacagt gacgatgcag gtgacagcca gaagctcaga ggagctgcca 3301 gtagatatca tcttggcatc cgtgggcCTC GAGTACCCAT ACGACGTACC TGATTATGCA 3361 ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA 3421 TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 3481 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 3541 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 3601 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 3661 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 3721 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 3781 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC 3841 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 3901 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 3961 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG 4021 CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA 4081 AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT 4141 TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC 4201 CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG 4261 GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCAGATCTGC 4321 GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 4381 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 4441 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 4501 GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 4561 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 4621 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 4681 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA 4741 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 4801 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 4861 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 4921 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT 4981 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 5041 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 5101 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA 5161 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 5221 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC 5281 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA 5341 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 5401 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT 5461 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 5521 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC 5581 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT 5641 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 5701 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG 5761 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 5821 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT 5881 TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA 5941 GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG 6001 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 6061 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 6121 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 6181 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 6241 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 6301 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 6361 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 6421 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 6481 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 6541 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 6601 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 6661 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 6721 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 6781 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 6841 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 6901 CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 6961 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 7021 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 7081 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 7141 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 7201 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 7261 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 7321 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 7381 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 7441 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 7501 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 7561 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 7621 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 7681 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 7741 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 7801 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 7861 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 7921 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 7981 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 8041 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 8101 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 8161 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 8221 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 8281 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 8341 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 8401 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 8461 CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTCGAC 8521 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT 8581 GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC 8641 CGACAATTGC ATGAAGAATC TGCTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.