pLV3-CMV-CREB3L2-EF1a-CopGFP-P

pLV3-CMV-CREB3L2-EF1a-CopGFP-Puro plasmid

PVT93918

pLV3-CMV-CREB3L2-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CREB3L2(human)-EF1a-CopGFP-Puro
Alias: NM_194071.4;BBF2H7
Gene lenght: 1563bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CREB3L2-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-CREB3L2-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-CREB3L2-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aggtgctgga gagcggggag 2341 cagggcgtgc tgcagtggga ccgcaagctg agcgagctgt cagagcccgg ggacggcgag 2401 gccctcatgt accacacgca cttctcagaa cttctggatg agttttccca gaacgtcttg 2461 ggtcagctcc tgaatgatcc tttcctctca gagaagagtg tgtcaatgga ggtggaacct 2521 tccccgacgt ccccggcgcc tctcatccag gctgagcaca gctactccct gtgcgaggag 2581 cctcgggccc agtcgccctt cacccacatt accaccagtg acagcttcaa tgacgatgag 2641 gtggaaagtg agaaatggta cctgtctaca gacttccctt caacatccat caagacagag 2701 ccagttacag acgaaccacc cccaggactc gttccgtctg tcactctgac catcacagcc 2761 atctccaccc cgttggaaaa ggaggaacct cctctggaaa tgaacactgg ggttgattcc 2821 tcgtgccaga ccattattcc taaaattaag ctggagcctc atgaagtgga tcagtttcta 2881 aacttctctc ctaaagaagc cccagtggac cacctgcatt tgccgcccac ccctccgagc 2941 agtcacggca gtgactcaga gggcagcctg agtcccaacc cacgcctgca ccccttcagc 3001 ctgcctcaga cccacagccc ctccagagct gcaccccggg ccccctccgc cctctccagc 3061 tcccctctcc tcacggctcc tcataaactg cagggatcag gccctctggt cctgacagag 3121 gaggagaaga ggaccctgat cgctgagggc tatcccatcc ccaccaaatt gcccctgtca 3181 aaatcagagg agaaggccct gaagaaaatt cggaggaaga tcaagaataa gatttctgct 3241 caggaaagta ggagaaagaa gaaagaatac atggacagcc tggagaaaaa agtggagtct 3301 tgttcaactg agaacttgga gcttcggaag aaggtagagg ttctagagaa cactaatagg 3361 actctccttc agcaactcca gaagcttcag actttggtga tgggcaaggt ttctcgaacc 3421 tgcaagttag ctggcacgca gactggcacc tgcctcatgg ttgtggtgct gtgctttgcc 3481 gttgcattcg gcagcttctt tcaaggctac gggccctatc cttctgccac caagatggct 3541 ctgcccagcc agcattccct gcaggagccc tacacagcct ccgtggtgag atccagaaac 3601 ctgctgatct acgaggaaca ttctccccca gaggagtcat ccagcccggg ctcggctggg 3661 gagctggggg gctgggatag aggttcctcc ctgctcaggg tgtcagggct ggagtccagg 3721 ccggatgtgg atcttcccca tttcattatc tcgaatgaga ccagcctgga gaagtcagtg 3781 cttttggagc tgcagcagca cctggtcagc gccaaactgg aggggaatga aacactaaaa 3841 gttgtagaac tcgacagaag agtgaacacc actttctaag gatccCTGCG ATATCCAGCg 3901 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3961 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 4021 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 4081 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 4141 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4201 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 4261 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 4321 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 4381 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4441 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 4501 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4561 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4621 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4681 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 4741 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4801 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4861 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4921 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4981 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 5041 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 5101 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 5161 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 5221 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 5281 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 5341 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 5401 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5461 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5521 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5581 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5641 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5701 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5761 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5821 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5881 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5941 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6001 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6061 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6121 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6181 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6241 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 6301 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 6361 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc 6421 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga 6481 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 6541 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 6601 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6661 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6721 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 6781 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6841 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6961 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 7021 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 7081 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 7141 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7261 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7321 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7381 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7441 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7501 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7561 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7621 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7681 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7741 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7801 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7861 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7921 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7981 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 8041 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8101 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8161 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8221 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8281 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8341 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8401 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8461 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8521 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8581 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8641 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8701 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8761 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8821 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8881 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8941 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9001 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9061 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9121 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9181 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9241 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9301 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 9361 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 9421 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 9481 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 9541 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 9601 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 9661 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 9721 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 9781 tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.