pLV3-CMV-AURKB-3×FLAG-EF1a-mCh

pLV3-CMV-AURKB-3×FLAG-EF1a-mCherry-Blast plasmid

PVT93606

pLV3-CMV-AURKB-3×FLAG-EF1a-mCherry-Blast plasmid Information

Plasmid name: pLV3-CMV-AURKB(human)-3×FLAG-EF1a-mCherry-Blast
Alias: NM_004217.4;AIK2; AIM-1; AIM1; ARK-2; ARK2; AurB;aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK-1; STK12; STK5
Gene lenght: 1032bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-AURKB-3×FLAG-EF1a-mCherry-Blast plasmid Description

pLV3-CMV-AURKB-3×FLAG-EF1a-mCherry-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-AURKB-3×FLAG-EF1a-mCherry-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggccca gaaggagaac tcctacccct ggccctacgg 2341 ccgacagacg gctccatctg gcctgagcac cctgccccag cgagtcctcc ggaaagagcc 2401 tgtcacccca tctgcacttg tcctcatgag ccgctccaat gtccagccca cagctgcccc 2461 tggccagaag gtgatggaga atagcagtgg gacacccgac atcttaacgc ggcacttcac 2521 aattgatgac tttgagattg ggcgtcctct gggcaaaggc aagtttggaa acgtgtactt 2581 ggctcgggag aagaaaagcc atttcatcgt ggcgctcaag gtcctcttca agtcccagat 2641 agagaaggag ggcgtggagc atcagctgcg cagagagatc gaaatccagg cccacctgca 2701 ccatcccaac atcctgcgtc tctacaacta tttttatgac cggaggagga tctacttgat 2761 tctagagtat gccccccgcg gggagctcta caaggagctg cagaagagct gcacatttga 2821 cgagcagcga acagccacga tcatggagga gttggcagat gctctaatgt actgccatgg 2881 gaagaaggtg attcacagag acataaagcc agaaaatctg ctcttagggc tcaagggaga 2941 gctgaagatt gctgacttcg gctggtctgt gcatgcgccc tccctgagga ggaagacaat 3001 gtgtggcacc ctggactacc tgcccccaga gatgattgag gggcgcatgc acaatgagaa 3061 ggtggatctg tggtgcattg gagtgctttg ctatgagctg ctggtgggga acccaccctt 3121 tgagagtgca tcacacaacg agacctatcg ccgcatcgtc aaggtggacc taaagttccc 3181 cgcttccgtg cccatgggag cccaggacct catctccaaa ctgctcaggc ataacccctc 3241 ggaacggctg cccctggccc aggtctcagc ccacccttgg gtccgggcca actctcggag 3301 ggtgctgcct ccctctgccc ttcaatctgt cgccggatcc GACTACAAAG ACCATGACGG 3361 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag 3421 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 3481 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa 3541 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 3601 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 3661 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg 3721 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc 3781 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc 3841 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact 3901 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc 3961 tacgctagac gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga 4021 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 4081 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 4141 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 4201 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 4261 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 4321 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 4381 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 4441 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 4501 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 4561 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 4621 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 4681 caagggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc 4741 cggccctATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC 4801 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG 4861 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA 4921 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC 4981 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT 5041 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT 5101 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGTCG ACaatcaacc 5161 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5221 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5281 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5341 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5401 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5461 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5521 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 5581 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5641 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg 5701 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat 5761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 5821 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 5881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6241 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc 6301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6361 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat 6421 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 6481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 6541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6601 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 6781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 6841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 6901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 6961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7021 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7261 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8581 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 8641 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 8701 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 8761 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 8821 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 8881 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 8941 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 9001 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.