pLV2-CMV-IMPA1-EGFP-IRES-Puro

pLV2-CMV-IMPA1-EGFP-IRES-Puro plasmid

PVT93430

pLV2-CMV-IMPA1-EGFP-IRES-Puro plasmid Information

Plasmid name: pLV2-CMV-IMPA1(mouse)-EGFP-IRES-Puro
Alias: NM_018864.6;2610002K09Rik; 2900059K10Rik
Gene lenght: 831bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-IMPA1-EGFP-IRES-Puro plasmid Description

pLV2-CMV-IMPA1-EGFP-IRES-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-IMPA1-EGFP-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcGC CACCatggca 2821 gacccttggc aggagtgcat ggactatgca gtaatcctcg cgaggcaagc tggagagatg 2881 attcgtgaag ctttaaaaaa tgagatggat gtcatgatta aaagttctcc agccgacttg 2941 gtaacagtta ctgaccaaaa agttgaaaaa atgctcatgt cttctataaa ggaaaagtat 3001 ccatgtcaca gcttcattgg tgaagagtct gtggcagctg gggagaagac ggtcttcaca 3061 gagcagccca cgtgggtcat tgaccccatt gatggaacga ctaacttcgt gcatcggttt 3121 ccctttgtag ctgtttcaat tggcttcctt gtgaataaag agatggagtt tggaattgtg 3181 tacagctgtg tggaagataa gatgtacacc ggcaggaaag ggaaaggtgc cttttgtaac 3241 ggtcagaagc ttcaggtgtc ccagcaggaa gacattacca agtcactctt ggtgaccgag 3301 ttgggctcgt ccagaaagcc cgagacttta cggatcgttc tctccaacat ggaaaagctg 3361 tgttccatcc ccatccatgg aatccggagt gttggaacag ctgctgttaa tatgtgcctt 3421 gtggcaacgg gaggagcaga tgcctattat gagatgggaa tccactgctg ggacatggcg 3481 ggagctggca tcattgtcac cgaggcaggc ggagtgctca tggatgtcac gggtggaccg 3541 ttcgatctga tgtctcggag aataattgcc gcaaatagta taacattagc caaaagaata 3601 gccaaagaaa ttgagataat acctttgcaa agagacgacg aaagcctcga gGGCGGTGGA 3661 GGAAGCggcg gaggtggatc tatggtgagc aagggcgagg agctgttcac cggggtggtg 3721 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 3781 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 3841 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 3901 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 3961 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 4021 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 4081 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 4141 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 4201 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 4261 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 4321 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 4381 atggacgagc tgtacaagta gactagttct agagcggccg cggatcccgc ccctctccct 4441 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 4501 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 4561 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 4621 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 4681 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 4741 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 4801 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 4861 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta 4921 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 4981 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac 5041 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac 5101 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca 5161 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 5221 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 5281 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 5341 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 5401 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 5461 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 5521 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 5581 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagacgcg 5641 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5701 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5761 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5821 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5881 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 5941 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 6001 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 6061 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 6121 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 6181 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 6241 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc 6301 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc 6361 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 6421 ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat 6481 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc 6541 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga 6601 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag 6661 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct 6721 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg 6781 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac 6841 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt 6901 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6961 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 7021 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 7081 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 7141 gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat 7201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 7261 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 7321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 7381 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 8041 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 8101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 8161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 8221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 8281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 8341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 8401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 8461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 9001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 9061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 9121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 9181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 9241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 9301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 9361 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat 9421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 9481 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat 9541 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta 9601 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata 9661 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact 9721 tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.