pLV3-CMV-3×HA-FAU-EF1a-CopGFP-

pLV3-CMV-3×HA-FAU-EF1a-CopGFP-Puro plasmid

PVT93142

pLV3-CMV-3×HA-FAU-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-FAU(human)-EF1a-CopGFP-Puro
Alias: NM_001997.5;asr1; eS30; FAU1; Fub1; Fubi; MNSFbeta;RPS30; S30;
Gene lenght: 402bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-FAU-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-3×HA-FAU-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-3×HA-FAU-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGG CGGCGTACCC ATACGACGTA 2341 CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC 2401 CCAGACTACG CATCAGCGGA AAAGCTTatg cagctctttg tccgcgccca ggagctacac 2461 accttcgagg tgaccggcca ggaaacggtc gcccagatca aggctcatgt agcctcactg 2521 gagggcattg ccccggaaga tcaagtcgtg ctcctggcag gcgcgcccct ggaggatgag 2581 gccactctgg gccagtgcgg ggtggaggcc ctgactaccc tggaagtagc aggccgcatg 2641 cttggaggta aagtccatgg ttccctggcc cgtgctggaa aagtgagagg tcagactcct 2701 aaggtggcca aacaggagaa gaagaagaag aagacaggtc gggctaagcg gcggatgcag 2761 tacaaccggc gctttgtcaa cgttgtgccc acctttggca agaagaaggg ccccaatgcc 2821 aactcttaag gatccCTGCG ATATCCAGCg cggccgcGaa ggatctgcga tcgctccggt 2881 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 2941 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3001 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3061 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3121 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3181 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3241 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3301 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 3361 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 3421 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 3481 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 3541 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 3601 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 3661 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 3721 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 3781 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 3841 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 3901 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 3961 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4021 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4081 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4141 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4201 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4261 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4321 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4381 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4441 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4501 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 4561 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 4621 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 4681 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 4741 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 4801 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 4861 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 4921 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 4981 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5041 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5101 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5161 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5221 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5281 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5341 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt 5401 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 5461 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 5521 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 5581 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 5641 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 5701 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 5761 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 5821 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 5881 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 5941 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 6001 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 6061 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 6121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6181 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 6361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 6961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 8281 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 8341 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 8401 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 8461 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 8521 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 8581 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 8641 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 8701 gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.