pLV3-CMV-TPX2-6×His-Fluc-hyg p

pLV3-CMV-TPX2-6×His-Fluc-hyg plasmid

PVT93117

pLV3-CMV-TPX2-6×His-Fluc-hyg plasmid Information

Plasmid name: pLV3-CMV-TPX2(human)-6×His-Fluc-hyg
Alias: NM_012112.5;C20orf1; C20orf2; DIL-2; DIL2; FLS353;GD:C20orf1; HCA519; HCTP4; p100; REPP86
Gene lenght: 2241bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TPX2-6×His-Fluc-hyg plasmid Description

pLV3-CMV-TPX2-6×His-Fluc-hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-TPX2-6×His-Fluc-hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgccacc atgtcacaag ttaaaagctc ttattcctat gatgccccct cggatttcat 2341 caatttttca tccttggatg atgaaggaga tactcaaaac atagattcat ggtttgagga 2401 gaaggccaat ttggagaata agttactggg gaagaatgga actggagggc tttttcaggg 2461 caaaactcct ttgagaaagg ctaatcttca gcaagctatt gtcacacctt tgaaaccagt 2521 tgacaacact tactacaaag aggcagaaaa agaaaatctt gtggaacaat ccattccgtc 2581 aaatgcttgt tcttccctgg aagttgaggc agccatatca agaaaaactc cagcccagcc 2641 tcagagaaga tctcttaggc tttctgctca gaaggatttg gaacagaaag aaaagcatca 2701 tgtaaaaatg aaagccaaga gatgtgccac tcctgtaatc atcgatgaaa ttctaccctc 2761 taagaaaatg aaagtttcta acaacaaaaa gaagccagag gaagaaggca gtgctcatca 2821 agatactgct gaaaagaatg catcttcccc agagaaagcc aagggtagac atactgtgcc 2881 ttgtatgcca cctgcaaagc agaagtttct aaaaagtact gaggagcaag agctggagaa 2941 gagtatgaaa atgcagcaag aggtggtgga gatgcggaaa aagaatgaag aattcaagaa 3001 acttgctctg gctggaatag ggcaacctgt gaagaaatca gtgagccagg tcaccaaatc 3061 agttgacttc cacttccgca cagatgagcg aatcaaacaa catcctaaga accaggagga 3121 atataaggaa gtgaacttta catctgaact acgaaagcat ccttcatctc ctgcccgagt 3181 gactaaggga tgtaccattg ttaagccttt caacctgtcc caaggaaaga aaagaacatt 3241 tgatgaaaca gtttctacat atgtgcccct tgcacagcaa gttgaagact tccataaacg 3301 aacccctaac agatatcatt tgaggagcaa gaaggatgat attaacctgt taccctccaa 3361 atcttctgtg accaagattt gcagagaccc acagactcct gtactgcaaa ccaaacaccg 3421 tgcacgggct gtgacctgca aaagtacagc agagctggag gctgaggagc tcgagaaatt 3481 gcaacaatac aaattcaaag cacgtgaact tgatcccaga atacttgaag gtgggcccat 3541 cttgcccaag aaaccacctg tgaaaccacc caccgagcct attggctttg atttggaaat 3601 tgagaaaaga atccaggagc gagaatcaaa gaagaaaaca gaggatgaac actttgaatt 3661 tcattccaga ccttgcccta ctaagatttt ggaagatgtt gtgggtgttc ctgaaaagaa 3721 ggtacttcca atcaccgtcc ccaagtcacc agcctttgca ttgaagaaca gaattcgaat 3781 gcccaccaaa gaagatgagg aagaggacga accggtagtg ataaaagctc aacctgtgcc 3841 acattatggg gtgcctttta agccccaaat cccagaggca agaactgtgg aaatatgccc 3901 tttctcgttt gattctcgag acaaagaacg tcagttacag aaggagaaga aaataaaaga 3961 actgcagaaa ggggaggtgc ccaagttcaa ggcacttccc ttgcctcatt ttgacaccat 4021 taacctgcca gagaagaagg taaagaatgt gacccagatt gaacctttct gcttggagac 4081 tgacagaaga ggtgctctga aggcacagac ttggaagcac cagctggaag aagaactgag 4141 acagcagaaa gaagcagctt gtttcaaggc tcgtccaaac accgtcatct ctcaggagcc 4201 ctttgttccc aagaaagaga agaaatcagt tgctgagggc ctttctggtt ctctagttca 4261 ggaacctttt cagctggcta ctgagaagag agccaaagag cggcaggagc tggagaagag 4321 aatggctgag gtagaagccc agaaagccca gcagttggag gaggccagac tacaggagga 4381 agagcagaaa aaagaggagc tggccaggct acggagagaa ctggtgcata aggcaaatcc 4441 aatacgcaag taccagggtc tggagataaa gtcaagtgac cagcctctga ctgtgcctgt 4501 atctcccaaa ttctccactc gattccactg cCATCACCAT CACCATCACT AGgcggccgc 4561 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc 4621 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt 4681 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc 4741 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac 4801 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg 4861 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac 4921 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct 4981 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca 5041 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc 5101 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgatggaag acgccaaaaa 5161 cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact 5221 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca 5281 tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc 5341 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct 5401 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa 5461 cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt 5521 gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat 5581 ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac 5641 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt 5701 cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa 5761 aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat 5821 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg 5881 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta 5941 tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct 6001 gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt 6061 atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc 6121 ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac 6181 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt 6241 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca 6301 aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga 6361 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg 6421 ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg 6481 ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga 6541 cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt 6601 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt 6661 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct 6721 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg 6781 aaagatcgcc gtgagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg 6841 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga 6901 ggagaatccc ggccctatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 6961 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 7021 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 7081 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 7141 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc 7201 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 7261 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 7321 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 7381 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 7441 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 7501 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 7561 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 7621 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 7681 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 7741 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 7801 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 7861 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 7921 tcgtccgagg gcaaaggaat aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag 7981 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 8041 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 8101 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 8161 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 8221 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 8281 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 8341 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 8401 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 8461 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct 8521 ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 8581 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata 8641 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 8701 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 8761 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 8821 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 8881 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 8941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 9001 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 9061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 9121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 9181 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt 9241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 10021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 10081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 11041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11341 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 11401 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 11461 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 11521 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 11581 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 11641 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc 11701 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 11761 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 11821 cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.