pCMV-mCherry-PRKAA1-3×Myc-Neo

pCMV-mCherry-PRKAA1-3×Myc-Neo plasmid

PVT96967

pCMV-mCherry-PRKAA1-3×Myc-Neo plasmid Information

Plasmid name: pCMV-mCherry-PRKAA1(human)-3×Myc-Neo
Alias: NM_006251.6;AMPK; AMPK alpha 1; AMPKa1
Gene lenght: 1677bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-PRKAA1-3×Myc-Neo plasmid Description

pCMV-mCherry-PRKAA1-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-PRKAA1-3×Myc-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tgGaGGTGGA GGTAGTGGAG GAGGCGGAAG Caagcttatg 1381 cgcagactca gttcctggag aaagatggcg acagccgaga agcagaaaca cgacgggcgg 1441 gtgaagatcg gccactacat tctgggtgac acgctggggg tcggcacctt cggcaaagtg 1501 aaggttggca aacatgaatt gactgggcat aaagtagctg tgaagatact caatcgacag 1561 aagattcgga gccttgatgt ggtaggaaaa atccgcagag aaattcagaa cctcaagctt 1621 ttcaggcatc ctcatataat taaactgtac caggtcatca gtacaccatc tgatattttc 1681 atggtgatgg aatatgtctc aggaggagag ctatttgatt atatctgtaa gaatggaagg 1741 ctggatgaaa aagaaagtcg gcgtctgttc caacagatcc tttctggtgt ggattattgt 1801 cacaggcata tggtggtcca tagagatttg aaacctgaaa atgtcctgct tgatgcacac 1861 atgaatgcaa agatagctga ttttggtctt tcaaacatga tgtcagatgg tgaattttta 1921 agaacaagtt gtggctcacc caactatgct gcaccagaag taatttcagg aagattgtat 1981 gcaggcccag aggtagatat atggagcagt ggggttattc tctatgcttt attatgtgga 2041 acccttccat ttgatgatga ccatgtgcca actcttttta agaagatatg tgatgggatc 2101 ttctataccc ctcaatattt aaatccttct gtgattagcc ttttgaaaca tatgctgcag 2161 gtggatccca tgaagagggc cacaatcaaa gatatcaggg aacatgaatg gtttaaacag 2221 gaccttccaa aatatctctt tcctgaggat ccatcatata gttcaaccat gattgatgat 2281 gaagccttaa aagaagtatg tgaaaagttt gagtgctcag aagaggaagt tctcagctgt 2341 ctttacaaca gaaatcacca ggatcctttg gcagttgcct accatctcat aatagataac 2401 aggagaataa tgaatgaagc caaagatttc tatttggcga caagcccacc tgattctttt 2461 cttgatgatc atcacctgac tcggccccat cctgaaagag taccattctt ggttgctgaa 2521 acaccaaggg cacgccatac ccttgatgaa ttaaatccac agaaatccaa acaccaaggt 2581 gtaaggaaag caaaatggca tttaggaatt agaagtcaaa gtcgaccaaa tgatattatg 2641 gcagaagtat gtagagcaat caaacaattg gattatgaat ggaaggttgt aaacccatat 2701 tatttgcgtg tacgaaggaa gaatcctgtg acaagcactt actccaaaat gagtctacag 2761 ttataccaag tggatagtag aacttatcta ctggatttcc gtagtattga tgatgaaatt 2821 acagaagcca aatcagggac tgctactcca cagagatcgg gatcagttag caactatcga 2881 tcttgccaaa ggagtgattc agatgctgag gctcaaggaa aatcctcaga agtttctctt 2941 acctcatctg tgacctcact tgactcttct cctgttgacc taactccaag acctggaagt 3001 cacacaatag aattttttga gatgtgtgca aatctaatta aaattcttgc acaagaattc 3061 GAACAAAAAC TAATATCAGA GGAAGATTTG GAACAGAAAC TAATCAGTGA AGAAGATTTA 3121 GAGCAAAAGT TAATTTCCGA GGAGGACTTA TAGgatccac cggatctaga taactgatca 3181 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 3241 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 3301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 3361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc 3421 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 3481 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 3541 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 3601 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 3661 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 3721 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 3781 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 3841 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 3901 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 3961 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt 4021 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 4081 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 4141 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 4201 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 4261 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 4321 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga 4381 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 4441 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 4501 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 4561 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 4621 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 4681 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 4741 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 4801 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 4861 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc 4921 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 4981 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 5041 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 5101 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 5161 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 5221 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 5281 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 5341 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag 5401 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg 5461 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc 5521 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg 5581 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact 5641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 5701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 5761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 5821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 5881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 5941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 6001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 6061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 6121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 6181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 6241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 6301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 6361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 6421 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 6481 ttaccgccat gcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.