pLV3-U6-EGR1-shRNA4-CopGFP-Pur

pLV3-U6-EGR1-shRNA4-CopGFP-Puro plasmid

PVT92769

pLV3-U6-EGR1-shRNA4-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-EGR1(human)-shRNA4-CopGFP-Puro
Alias: GTTACTACCTCTTATCCAT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-EGR1-shRNA4-CopGFP-Puro plasmid Description

pLV3-U6-EGR1-shRNA4-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-EGR1-shRNA4-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg GTTACTACCT CTTATCCATt tcaagagaAT 2221 GGATAAGAGG TAGTAACttt tttggatccg cggccgcGaa ggatctgcga tcgctccggt 2281 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 2341 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 2401 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 2461 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 2521 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 2581 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 2641 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 2701 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 2761 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 2821 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 2881 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 2941 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 3001 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 3061 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 3121 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 3181 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 3241 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 3301 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 3361 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 3421 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 3481 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 3541 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 3601 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 3661 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 3721 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 3781 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 3841 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 3901 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 3961 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 4021 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 4081 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 4141 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 4201 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 4261 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 4321 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 4381 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 4441 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 4501 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 4561 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 4621 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 4681 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 4741 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt 4801 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 4861 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 4921 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 4981 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 5041 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 5101 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 5161 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 5221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 5281 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 5341 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 5401 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 5461 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 5521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 5581 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 5641 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 5701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 5761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 5821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 5881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 5941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 6361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 6541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6661 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6721 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6781 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 6841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 6901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 6961 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7501 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 7561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 7621 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 7681 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 7741 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 7801 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 7861 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 7921 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 7981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 8041 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 8101 gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.