pLenti-U6-BECN1-sgRNA2-Cas9-Hy

pLenti-U6-BECN1-sgRNA2-Cas9-Hyg plasmid

PVT92751

pLenti-U6-BECN1-sgRNA2-Cas9-Hyg plasmid Information

Plasmid name: pLenti-U6-BECN1(human)-sgRNA2-Cas9-Hyg
Alias: CCTGGACCGTGTCACCATCC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLenti-U6-BECN1-sgRNA2-Cas9-Hyg plasmid Description

pLenti-U6-BECN1-sgRNA2-Cas9-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLenti-U6-BECN1-sgRNA2-Cas9-Hyg plasmid Sequence

1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGCGCGT 241 TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC 301 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC 361 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG 421 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 481 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC 541 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 601 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG 661 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT 721 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA 781 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GCGCGTTTTG CCTGTACTGG 841 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 901 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 961 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 1021 TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC TCGACGCAGG 1081 ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT GAGTACGCCA 1141 AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT CAGTATTAAG 1201 CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA 1261 TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC AGTTAATCCT 1321 GGCCTGTTAG AAACATCAGA AGGCTGTAGA CAAATACTGG GACAGCTACA ACCATCCCTT 1381 CAGACAGGAT CAGAAGAACT TAGATCATTA TATAATACAG TAGCAACCCT CTATTGTGTG 1441 CATCAAAGGA TAGAGATAAA AGACACCAAG GAAGCTTTAG ACAAGATAGA GGAAGAGCAA 1501 AACAAAAGTA AGACCACCGC ACAGCAAGCG GCCGCTGATC TTCAGACCTG GAGGAGGAGA 1561 TATGAGGGAC AATTGGAGAA GTGAATTATA TAAATATAAA GTAGTAAAAA TTGAACCATT 1621 AGGAGTAGCA CCCACCAAGG CAAAGAGAAG AGTGGTGCAG AGAGAAAAAA GAGCAGTGGG 1681 AATAGGAGCT TTGTTCCTTG GGTTCTTGGG AGCAGCAGGA AGCACTATGG GCGCAGCGTC 1741 AATGACGCTG ACGGTACAGG CCAGACAATT ATTGTCTGGT ATAGTGCAGC AGCAGAACAA 1801 TTTGCTGAGG GCTATTGAGG CGCAACAGCA TCTGTTGCAA CTCACAGTCT GGGGCATCAA 1861 GCAGCTCCAG GCAAGAATCC TGGCTGTGGA AAGATACCTA AAGGATCAAC AGCTCCTGGG 1921 GATTTGGGGT TGCTCTGGAA AACTCATTTG CACCACTGCT GTGCCTTGGA ATGCTAGTTG 1981 GAGTAATAAA TCTCTGGAAC AGATTTGGAA TCACACGACC TGGATGGAGT GGGACAGAGA 2041 AATTAACAAT TACACAAGCT TAATACACTC CTTAATTGAA GAATCGCAAA ACCAGCAAGA 2101 AAAGAATGAA CAAGAATTAT TGGAATTAGA TAAATGGGCA AGTTTGTGGA ATTGGTTTAA 2161 CATAACAAAT TGGCTGTGGT ATATAAAATT ATTCATAATG ATAGTAGGAG GCTTGGTAGG 2221 TTTAAGAATA GTTTTTGCTG TACTTTCTAT AGTGAATAGA GTTAGGCAGG GATATTCACC 2281 ATTATCGTTT CAGACCCACC TCCCAACCCC GAGGGGACCC GACAGGCCCG AAGGAATAGA 2341 AGAAGAAGGT GGAGAGAGAG ACAGAGACAG ATCCATTCGA TTAGTGAACG GATCGGCACT 2401 GCGTGCGCCA ATTCTGCAGA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG 2461 GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA 2521 CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA 2581 GCAGAGATCC AGTTTGGTTA ATTAAGGTAC CGAGGGCCTA TTTCCCATGA TTCCTTCATA 2641 TTTGCATATA CGATACAAGG CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC 2701 AAAGATATTA GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT 2761 TTTAAAATTA TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA 2821 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACACC GCCTGGACCG TGTCACCATC 2881 CGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG 2941 TGGCACCGAG TCGGTGCTTT TTTGAATTCG CTAGCTAGGT CTTGAAAGGA GTGGGAATTG 3001 GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG 3061 GAGGGGTCGG CAATTGATCC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG 3121 ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG 3181 TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GACCGGTTCT 3241 AGAGCGCTGC CACCATGGAC AAGAAGTACA GCATCGGCCT GGACATCGGC ACCAACTCTG 3301 TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG 3361 GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG 3421 GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA 3481 AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA 3541 GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC 3601 ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT 3661 ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC 3721 TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC 3781 CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT 3841 TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC 3901 TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG 3961 GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT 4021 TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG 4081 ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC 4141 TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC 4201 CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA 4261 AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA 4321 AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA 4381 TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG 4441 AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC 4501 TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA 4561 ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG 4621 CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT 4681 GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA GAGCTTCATC GAGCGGATGA 4741 CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC CAAGCACAGC CTGCTGTACG 4801 AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA CGTGACCGAG GGAATGAGAA 4861 AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA AGGCCATCGT GGACCTGCTG TTCAAGACCA 4921 ACCGGAAAGT GACCGTGAAG CAGCTGAAAG AGGACTACTT CAAGAAAATC GAGTGCTTCG 4981 ACTCCGTGGA AATCTCCGGC GTGGAAGATC GGTTCAACGC CTCCCTGGGC ACATACCACG 5041 ATCTGCTGAA AATTATCAAG GACAAGGACT TCCTGGACAA TGAGGAAAAC GAGGACATTC 5101 TGGAAGATAT CGTGCTGACC CTGACACTGT TTGAGGACAG AGAGATGATC GAGGAACGGC 5161 TGAAAACCTA TGCCCACCTG TTCGACGACA AAGTGATGAA GCAGCTGAAG CGGCGGAGAT 5221 ACACCGGCTG GGGCAGGCTG AGCCGGAAGC TGATCAACGG CATCCGGGAC AAGCAGTCCG 5281 GCAAGACAAT CCTGGATTTC CTGAAGTCCG ACGGCTTCGC CAACAGAAAC TTCATGCAGC 5341 TGATCCACGA CGACAGCCTG ACCTTTAAAG AGGACATCCA GAAAGCCCAG GTGTCCGGCC 5401 AGGGCGATAG CCTGCACGAG CACATTGCCA ATCTGGCCGG CAGCCCCGCC ATTAAGAAGG 5461 GCATCCTGCA GACAGTGAAG GTGGTGGACG AGCTCGTGAA AGTGATGGGC CGGCACAAGC 5521 CCGAGAACAT CGTGATCGAA ATGGCCAGAG AGAACCAGAC CACCCAGAAG GGACAGAAGA 5581 ACAGCCGCGA GAGAATGAAG CGGATCGAAG AGGGCATCAA AGAGCTGGGC AGCCAGATCC 5641 TGAAAGAACA CCCCGTGGAA AACACCCAGC TGCAGAACGA GAAGCTGTAC CTGTACTACC 5701 TGCAGAATGG GCGGGATATG TACGTGGACC AGGAACTGGA CATCAACCGG CTGTCCGACT 5761 ACGATGTGGA CCATATCGTG CCTCAGAGCT TTCTGAAGGA CGACTCCATC GACAACAAGG 5821 TGCTGACCAG AAGCGACAAG AACCGGGGCA AGAGCGACAA CGTGCCCTCC GAAGAGGTCG 5881 TGAAGAAGAT GAAGAACTAC TGGCGGCAGC TGCTGAACGC CAAGCTGATT ACCCAGAGAA 5941 AGTTCGACAA TCTGACCAAG GCCGAGAGAG GCGGCCTGAG CGAACTGGAT AAGGCCGGCT 6001 TCATCAAGAG ACAGCTGGTG GAAACCCGGC AGATCACAAA GCACGTGGCA CAGATCCTGG 6061 ACTCCCGGAT GAACACTAAG TACGACGAGA ATGACAAGCT GATCCGGGAA GTGAAAGTGA 6121 TCACCCTGAA GTCCAAGCTG GTGTCCGATT TCCGGAAGGA TTTCCAGTTT TACAAAGTGC 6181 GCGAGATCAA CAACTACCAC CACGCCCACG ACGCCTACCT GAACGCCGTC GTGGGAACCG 6241 CCCTGATCAA AAAGTACCCT AAGCTGGAAA GCGAGTTCGT GTACGGCGAC TACAAGGTGT 6301 ACGACGTGCG GAAGATGATC GCCAAGAGCG AGCAGGAAAT CGGCAAGGCT ACCGCCAAGT 6361 ACTTCTTCTA CAGCAACATC ATGAACTTTT TCAAGACCGA GATTACCCTG GCCAACGGCG 6421 AGATCCGGAA GCGGCCTCTG ATCGAGACAA ACGGCGAAAC CGGGGAGATC GTGTGGGATA 6481 AGGGCCGGGA TTTTGCCACC GTGCGGAAAG TGCTGAGCAT GCCCCAAGTG AATATCGTGA 6541 AAAAGACCGA GGTGCAGACA GGCGGCTTCA GCAAAGAGTC TATCCTGCCC AAGAGGAACA 6601 GCGATAAGCT GATCGCCAGA AAGAAGGACT GGGACCCTAA GAAGTACGGC GGCTTCGACA 6661 GCCCCACCGT GGCCTATTCT GTGCTGGTGG TGGCCAAAGT GGAAAAGGGC AAGTCCAAGA 6721 AACTGAAGAG TGTGAAAGAG CTGCTGGGGA TCACCATCAT GGAAAGAAGC AGCTTCGAGA 6781 AGAATCCCAT CGACTTTCTG GAAGCCAAGG GCTACAAAGA AGTGAAAAAG GACCTGATCA 6841 TCAAGCTGCC TAAGTACTCC CTGTTCGAGC TGGAAAACGG CCGGAAGAGA ATGCTGGCCT 6901 CTGCCGGCGA ACTGCAGAAG GGAAACGAAC TGGCCCTGCC CTCCAAATAT GTGAACTTCC 6961 TGTACCTGGC CAGCCACTAT GAGAAGCTGA AGGGCTCCCC CGAGGATAAT GAGCAGAAAC 7021 AGCTGTTTGT GGAACAGCAC AAGCACTACC TGGACGAGAT CATCGAGCAG ATCAGCGAGT 7081 TCTCCAAGAG AGTGATCCTG GCCGACGCTA ATCTGGACAA AGTGCTGTCC GCCTACAACA 7141 AGCACCGGGA TAAGCCCATC AGAGAGCAGG CCGAGAATAT CATCCACCTG TTTACCCTGA 7201 CCAATCTGGG AGCCCCTGCC GCCTTCAAGT ACTTTGACAC CACCATCGAC CGGAAGAGGT 7261 ACACCAGCAC CAAAGAGGTG CTGGACGCCA CCCTGATCCA CCAGAGCATC ACCGGCCTGT 7321 ACGAGACACG GATCGACCTG TCTCAGCTGG GAGGCGACAA GCGACCTGCC GCCACAAAGA 7381 AGGCTGGACA GGCTAAGAAG AAGAAAGATT ACAAAGACGA TGACGATAAG GGATCCGGCG 7441 CAACAAACTT CTCTCTGCTG AAACAAGCCG GAGATGTCGA AGAGAATCCT GGACCGAAAA 7501 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTGT 7561 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG 7621 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG 7681 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT 7741 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC 7801 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG 7861 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC 7921 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC 7981 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC 8041 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA 8101 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG 8161 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG 8221 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC 8281 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT 8341 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA 8401 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT GGCTGTGTAG 8461 AAGTACTTCG CCGATAGACG CGTTAAGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG 8521 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 8581 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 8641 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 8701 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 8761 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 8821 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 8881 CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 8941 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 9001 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT 9061 CCCTTTGGGC CGCCTCCCCG CGTCGACTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA 9121 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG 9181 ACAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 9241 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 9301 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 9361 TTAGTCAGTG TGGAAAATCT CTAGCAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG 9421 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG 9481 AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA 9541 GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG 9601 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 9661 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 9721 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 9781 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 9841 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 9901 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 9961 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 10021 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 10081 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 10141 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 10201 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 10261 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT 10321 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 10381 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 10441 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA 10501 GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG 10561 AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC 10621 GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC 10681 GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG 10741 GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC 10801 GAGTGGTCGG AGGTCGTGTC CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG 10861 ATCGGCGAGC AGCCGTGGGG GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG 10921 CACTTCGTGG CCGAGGAGCA GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC 10981 TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG 11041 CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT 11101 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT 11161 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC 11221 TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 11281 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 11341 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 11401 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 11461 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 11521 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 11581 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 11641 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 11701 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 11761 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 11821 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 11881 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 11941 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 12001 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 12061 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 12121 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 12181 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 12241 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 12301 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 12361 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 12421 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 12481 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 12541 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 12601 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 12661 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 12721 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 12781 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 12841 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 12901 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 12961 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 13021 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 13081 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 13141 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 13201 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 13261 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 13321 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 13381 CCCCGAAAAG TGCCACCTGA C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.