pLV3-CMV-NKIRAS1-3×FLAG-CopGFP

pLV3-CMV-NKIRAS1-3×FLAG-CopGFP-Puro plasmid

PVT92713

pLV3-CMV-NKIRAS1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NKIRAS1(human)-3×FLAG-CopGFP-Puro
Alias: NM_020345.4;kappaB-Ras1; KBRAS1
Gene lenght: 576bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-NKIRAS1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NKIRAS1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-NKIRAS1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg gaaagggctg caaggttgtg 2341 gtttgtggat tgttatctgt ggggaaaact gcaattttgg agcagctcct ttatggaaat 2401 catactattg gaatggaaga ttgcgaaaca atggaagatg tatacatggc ttcagtagaa 2461 acagaccgag gagtaaaaga acagttacat ctttatgaca ccagaggtct acaggaaggc 2521 gtggagctgc caaagcatta tttttcattt gctgatggct tcgttcttgt gtacagtgtg 2581 aataaccttg aatcctttca aagagtggag cttctgaaga aagaaatcga taagttcaaa 2641 gacaaaaaag aggtagcaat tgtggtatta ggaaacaaaa tcgacctttc tgagcagaga 2701 caagtggacg ctgaagtggc acagcagtgg gcaaaaagtg agaaagtaag actgtgggag 2761 gtgactgtta cagatcggaa aactctgatt gaaccattca ctttattagc cagtaaactt 2821 tctcaacccc agagcaaatc aagctttcct ttgcctggga ggaaaaacaa agggaactct 2881 aattctgaga acggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 2941 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 3001 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3061 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3121 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3181 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 3241 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 3301 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 3361 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 3421 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 3481 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 3541 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 3601 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 3661 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 3721 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 3781 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 3841 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 3901 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 3961 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 4021 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 4081 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 4141 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 4201 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 4261 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 4321 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 4381 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4441 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4501 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4561 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4621 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4681 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4741 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4801 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 4861 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 4921 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 4981 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5041 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5101 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5161 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5221 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 5281 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 5341 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5401 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5461 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 5521 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 5581 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 5641 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 5701 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 5761 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 5821 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 5881 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 5941 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 6001 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 6061 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6121 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 6181 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 6241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 6421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 6481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7081 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 7381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 7441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 7501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 8221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 8341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 8401 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 8461 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 8521 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 8581 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 8641 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 8701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 8761 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 8821 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.