pLV3-CMV-FLNA-3×FLAG-CopGFP-Pu

pLV3-CMV-FLNA-3×FLAG-CopGFP-Puro plasmid

PVT92681

pLV3-CMV-FLNA-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FLNA(human)-3×FLAG-CopGFP-Puro
Alias: NM_001110556.2;ABP-280; ABPX; CSBS; CVD1; FGS2; FLN;FLN-A; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; XLVD; XMVD;
Gene lenght: 7941bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-FLNA-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FLNA-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FLNA-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga gtagctccca ctctcgggcg 2341 ggccagagcg cagcaggcgc ggctccgggc ggcggcgtcg acacgcggga cgccgagatg 2401 ccggccaccg agaaggacct ggcggaggac gcgccgtgga agaagatcca gcagaacact 2461 ttcacgcgct ggtgcaacga gcacctgaag tgcgtgagca agcgcatcgc caacctgcag 2521 acggacctga gcgacgggct gcggcttatc gcgctgttgg aggtgctcag ccagaagaag 2581 atgcaccgca agcacaacca gcggcccact ttccgccaaa tgcagcttga gaacgtgtcg 2641 gtggcgctcg agttcctgga ccgcgagagc atcaaactgg tgtccatcga cagcaaggcc 2701 atcgtggacg ggaacctgaa gctgatcctg ggcctcatct ggaccctgat cctgcactac 2761 tccatctcca tgcccatgtg ggacgaggag gaggatgagg aggccaagaa gcagaccccc 2821 aagcagaggc tcctgggctg gatccagaac aagctgccgc agctgcccat caccaacttc 2881 agccgggact ggcagagcgg ccgggccctg ggcgccctgg tggacagctg tgccccgggc 2941 ctgtgtcctg actgggactc ttgggacgcc agcaagcccg ttaccaatgc gcgagaggcc 3001 atgcagcagg cggatgactg gctgggcatc ccccaggtga tcacccccga ggagattgtg 3061 gaccccaacg tggacgagca ctctgtcatg acctacctgt cccagttccc caaggccaag 3121 ctgaagccag gggctccctt gcggcccaaa ctgaacccga agaaagcccg tgcctacggg 3181 ccaggcatcg agcccacagg caacatggtg aagaagcggg cagagttcac tgtggagacc 3241 agaagtgctg gccagggaga ggtgctggtg tacgtggagg acccggccgg acaccaggag 3301 gaggcaaaag tgaccgccaa taacgacaag aaccgcacct tctccgtctg gtacgtcccc 3361 gaggtgacgg ggactcataa ggttactgtg ctctttgctg gccagcacat cgccaagagc 3421 cccttcgagg tgtacgtgga taagtcacag ggtgacgcca gcaaagtgac agcccaaggt 3481 cccggcctgg agcccagtgg caacatcgcc aacaagacca cctactttga gatctttacg 3541 gcaggagctg gcacgggcga ggtcgaggtt gtgatccagg accccatggg acagaagggc 3601 acggtagagc ctcagctgga ggcccggggc gacagcacat accgctgcag ctaccagccc 3661 accatggagg gcgtccacac cgtgcacgtc acgtttgccg gcgtgcccat ccctcgcagc 3721 ccctacactg tcactgttgg ccaagcctgt aacccgagtg cctgccgggc ggttggccgg 3781 ggcctccagc ccaagggtgt gcgggtgaag gagacagctg acttcaaggt gtacacaaag 3841 ggcgctggca gtggggagct gaaggtcacc gtgaagggcc ccaagggaga ggagcgcgtg 3901 aagcagaagg acctggggga tggcgtgtat ggcttcgagt attaccccat ggtccctgga 3961 acctatatcg tcaccatcac gtggggtggt cagaacatcg ggcgcagtcc cttcgaagtg 4021 aaggtgggca ccgagtgtgg caatcagaag gtacgggcct ggggccctgg gctggagggc 4081 ggcgtcgttg gcaagtcagc agactttgtg gtggaggcta tcggggacga cgtgggcacg 4141 ctgggcttct cggtggaagg gccatcgcag gctaagatcg aatgtgacga caagggcgac 4201 ggctcctgtg atgtgcgcta ctggccgcag gaggctggcg agtatgccgt tcacgtgctg 4261 tgcaacagcg aagacatccg cctcagcccc ttcatggctg acatccgtga cgcgccccag 4321 gacttccacc cagacagggt gaaggcacgt gggcctggat tggagaagac aggtgtggcc 4381 gtcaacaagc cagcagagtt cacagtggat gccaagcacg gtggcaaggc cccacttcgg 4441 gtccaagtcc aggacaatga aggctgccct gtggaggcgt tggtcaagga caacggcaat 4501 ggcacttaca gctgctccta cgtgcccagg aagccggtga agcacacagc catggtgtcc 4561 tggggaggcg tcagcatccc caacagcccc ttcagggtga atgtgggagc tggcagccac 4621 cccaacaagg tcaaagtata cggccccgga gtagccaaga cagggctcaa ggcccacgag 4681 cccacctact tcactgtgga ctgcgccgag gctggccagg gggacgtcag catcggcatc 4741 aagtgtgccc ctggagtggt aggccccgcc gaagctgaca tcgacttcga catcatccgc 4801 aatgacaatg acaccttcac ggtcaagtac acgccccggg gggctggcag ctacaccatt 4861 atggtcctct ttgctgacca ggccacgccc accagcccca tccgagtcaa ggtggagccc 4921 tctcatgacg ccagtaaggt gaaggccgag ggccctggcc tcagtcgcac tggtgtcgag 4981 cttggcaagc ccacccactt cacagtaaat gccaaagctg ctggcaaagg caagctggac 5041 gtccagttct caggactcac caagggggat gcagtgcgag atgtggacat catcgaccac 5101 catgacaaca cctacacagt caagtacacg cctgtccagc agggtccagt aggcgtcaat 5161 gtcacttatg gaggggatcc catccctaag agccctttct cagtggcagt atctccaagc 5221 ctggacctca gcaagatcaa ggtgtctggc ctgggagaga aggtggacgt tggcaaagac 5281 caggagttca cagtcaaatc aaagggtgct ggtggtcaag gcaaagtggc atccaagatt 5341 gtgggcccct cgggtgcagc ggtgccctgc aaggtggagc caggcctggg ggctgacaac 5401 agtgtggtgc gcttcctgcc ccgtgaggaa gggccctatg aggtggaggt gacctatgac 5461 ggcgtgcccg tgcctggcag cccctttcct ctggaagctg tggcccccac caagcctagc 5521 aaggtgaagg cgtttgggcc ggggctgcag ggaggcagtg cgggctcccc cgcccgcttc 5581 accatcgaca ccaagggcgc cggcacaggt ggcctgggcc tgacggtgga gggcccctgt 5641 gaggcgcagc tcgagtgctt ggacaatggg gatggcacat gttccgtgtc ctacgtgccc 5701 accgagcccg gggactacaa catcaacatc ctcttcgctg acacccacat ccctggctcc 5761 ccattcaagg cccacgtggt tccctgcttt gacgcatcca aagtcaagtg ctcaggcccc 5821 gggctggagc gggccaccgc tggggaggtg ggccaattcc aagtggactg ctcgagcgcg 5881 ggcagcgcgg agctgaccat tgagatctgc tcggaggcgg ggcttccggc cgaggtgtac 5941 atccaggacc acggtgatgg cacgcacacc attacctaca ttcccctctg ccccggggcc 6001 tacaccgtca ccatcaagta cggcggccag cccgtgccca acttccccag caagctgcag 6061 gtggaacctg cggtggacac ttccggtgtc cagtgctatg ggcctggtat tgagggccag 6121 ggtgtcttcc gtgaggccac cactgagttc agtgtggacg cccgggctct gacacagacc 6181 ggagggccgc acgtcaaggc ccgtgtggcc aacccctcag gcaacctgac ggagacctac 6241 gttcaggacc gtggcgatgg catgtacaaa gtggagtaca cgccttacga ggagggactg 6301 cactccgtgg acgtgaccta tgacggcagt cccgtgccca gcagcccctt ccaggtgccc 6361 gtgaccgagg gctgcgaccc ctcccgggtg cgtgtccacg ggccaggcat ccaaagtggc 6421 accaccaaca agcccaacaa gttcactgtg gagaccaggg gagctggcac gggcggcctg 6481 ggcctggctg tagagggccc ctccgaggcc aagatgtcct gcatggataa caaggacggc 6541 agctgctcgg tcgagtacat cccttatgag gctggcacct acagcctcaa cgtcacctat 6601 ggtggccatc aagtgccagg cagtcctttc aaggtccctg tgcatgatgt gacagatgcg 6661 tccaaggtca agtgctctgg gcccggcctg agcccaggca tggttcgtgc caacctccct 6721 cagtccttcc aggtggacac aagcaaggct ggtgtggccc cattgcaggt caaagtgcaa 6781 gggcccaaag gcctggtgga gccagtggac gtggtagaca acgctgatgg cacccagacc 6841 gtcaattatg tgcccagccg agaagggccc tacagcatct cagtactgta tggagatgaa 6901 gaggtacccc ggagcccctt caaggtcaag gtgctgccta ctcatgatgc cagcaaggtg 6961 aaggccagtg gccccgggct caacaccact ggcgtgcctg ccagcctgcc cgtggagttc 7021 accatcgatg caaaggacgc cggggagggc ctgctggctg tccagatcac ggatcccgaa 7081 ggcaagccga agaagacaca catccaagac aaccatgacg gcacgtatac agtggcctac 7141 gtgccagacg tgacaggtcg ctacaccatc ctcatcaagt acggtggtga cgagatcccc 7201 ttctccccgt accgcgtgcg tgccgtgccc accggggacg ccagcaagtg cactgtcaca 7261 gtgtcaatcg gaggtcacgg gctaggtgct ggcatcggcc ccaccattca gattggggag 7321 gagacggtga tcactgtgga cactaaggcg gcaggcaaag gcaaagtgac gtgcaccgtg 7381 tgcacgcctg atggctcaga ggtggatgtg gacgtggtgg agaatgagga cggcactttc 7441 gacatcttct acacggcccc ccagccgggc aaatacgtca tctgtgtgcg ctttggtggc 7501 gagcacgtgc ccaacagccc cttccaagtg acggctctgg ctggggacca gccctcggtg 7561 cagccccctc tacggtctca gcagctggcc ccacagtaca cctacgccca gggcggccag 7621 cagacttggg ccccggagag gcccctggtg ggtgtcaatg ggctggatgt gaccagcctg 7681 aggccctttg accttgtcat ccccttcacc atcaagaagg gcgagatcac aggggaggtt 7741 cggatgccct caggcaaggt ggcgcagccc accatcactg acaacaaaga cggcaccgtg 7801 accgtgcggt atgcacccag cgaggctggc ctgcacgaga tggacatccg ctatgacaac 7861 atgcacatcc caggaagccc cttgcagttc tatgtggatt acgtcaactg tggccatgtc 7921 actgcctatg ggcctggcct cacccatgga gtagtgaaca agcctgccac cttcaccgtc 7981 aacaccaagg atgcaggaga ggggggcctg tctctggcca ttgagggccc gtccaaagca 8041 gaaatcagct gcactgacaa ccaggatggg acatgcagcg tgtcctacct gcctgtgctg 8101 ccgggggact acagcattct agtcaagtac aatgaacagc acgtcccagg cagccccttc 8161 actgctcggg tcacaggtga cgactccatg cgtatgtccc acctaaaggt cggctctgct 8221 gccgacatcc ccatcaacat ctcagagacg gatctcagcc tgctgacggc cactgtggtc 8281 ccgccctcgg gccgggagga gccctgtttg ctgaagcggc tgcgtaatgg ccacgtgggg 8341 atttcattcg tgcccaagga gacgggggag cacctggtgc atgtgaagaa aaatggccag 8401 cacgtggcca gcagccccat cccggtggtg atcagccagt cggaaattgg ggatgccagt 8461 cgtgttcggg tctctggtca gggccttcac gaaggccaca cctttgagcc tgcagagttt 8521 atcattgata cccgcgatgc aggctatggt gggctcagcc tgtccattga gggccccagc 8581 aaggtggaca tcaacacaga ggacctggag gacgggacgt gcagggtcac ctactgcccc 8641 acagagccag gcaactacat catcaacatc aagtttgccg accagcacgt gcctggcagc 8701 cccttctctg tgaaggtgac aggcgagggc cgggtgaaag agagcatcac ccgcaggcgt 8761 cgggctcctt cagtggccaa cgttggtagt cattgtgacc tcagcctgaa aatccctgaa 8821 attagcatcc aggatatgac agcccaggtg accagcccat cgggcaagac ccatgaggcc 8881 gagatcgtgg aaggggagaa ccacacctac tgcatccgct ttgttcccgc tgagatgggc 8941 acacacacag tcagcgtgaa gtacaagggc cagcacgtgc ctgggagccc cttccagttc 9001 accgtggggc ccctagggga agggggagcc cacaaggtcc gagctggggg ccctggcctg 9061 gagagagctg aagctggagt gccagccgaa ttcagtatct ggacccggga agctggtgct 9121 ggaggcctgg ccattgctgt cgagggcccc agcaaggctg agatctcttt tgaggaccgc 9181 aaggacggct cctgtggtgt ggcttatgtg gtccaggagc caggtgacta cgaagtctca 9241 gtcaagttca acgaggaaca cattcccgac agccccttcg tggtgcctgt ggcttctccg 9301 tctggcgacg cccgccgcct cactgtttct agccttcagg agtcagggct aaaggtcaac 9361 cagccagcct cttttgcagt cagcctgaac ggggccaagg gggcgatcga tgccaaggtg 9421 cacagcccct caggagccct ggaggagtgc tatgtcacag aaattgacca agataagtat 9481 gctgtgcgct tcatccctcg ggagaatggc gtttacctga ttgacgtcaa gttcaacggc 9541 acccacatcc ctggaagccc cttcaagatc cgagttgggg agcctgggca tggaggggac 9601 ccaggcttgg tgtctgctta cggagcaggt ctggaaggcg gtgtcacagg gaacccagct 9661 gagttcgtcg tgaacacgag caatgcggga gctggtgccc tgtcggtgac cattgacggc 9721 ccctccaagg tgaagatgga ttgccaggag tgccctgagg gctaccgcgt cacctatacc 9781 cccatggcac ctggcagcta cctcatctcc atcaagtacg gcggccccta ccacattggg 9841 ggcagcccct tcaaggccaa agtcacaggc ccccgtctcg tcagcaacca cagcctccac 9901 gagacatcat cagtgtttgt agactctctg accaaggcca cctgtgcccc ccagcatggg 9961 gccccgggtc ctgggcctgc tgacgccagc aaggtggtgg ccaagggcct ggggctgagc 10021 aaggcctacg taggccagaa gagcagcttc acagtagact gcagcaaagc aggcaacaac 10081 atgctgctgg tgggggttca tggcccaagg accccctgcg aggagatcct ggtgaagcac 10141 gtgggcagcc ggctctacag cgtgtcctac ctgctcaagg acaaggggga gtacacactg 10201 gtggtcaaat ggggggacga gcacatccca ggcagcccct accgcgttgt ggtgcccgga 10261 tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 10321 GATGACAAGt agcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 10381 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 10441 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 10501 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 10561 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 10621 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 10681 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 10741 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 10801 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 10861 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 10921 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 10981 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 11041 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 11101 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 11161 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 11221 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 11281 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 11341 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 11401 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 11461 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 11521 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 11581 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 11641 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 11701 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 11761 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 11821 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 11881 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 11941 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 12001 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 12061 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 12121 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 12181 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 12241 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 12301 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 12361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 12421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 12481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 12541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 12601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 12661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT 12721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 12781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 12841 ccgcgtctTc gccttcgccc tcagacgagt cggatctccc tttggGccgc ctccccgcct 12901 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 12961 aaagggggga ctggaagggc taattcactc ccaacgaaGa taagatctgc tttttgcttg 13021 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 13081 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 13141 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 13201 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 13261 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 13321 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 13381 actcatcaat gtatcttatc atgtctggct ctagctaTCC CGCCCCTAAC TCCGCCCAtc 13441 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 13501 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 13561 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc 13621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 13681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 13741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 13801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 13861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 13921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 13981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 14041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 14101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 14161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 14221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 14281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 14341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 14401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 14461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 14521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 14581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 14641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 14701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 14761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 14821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 14881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 14941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 15001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 15061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 15121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 15181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 15241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 15301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 15361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 15421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 15481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 15541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 15601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 15661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 15721 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 15781 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 15841 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 15901 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 15961 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 16021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 16081 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 16141 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 16201 acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.