pCMV-3×FLAG-SLC32A1-Neo plasmi

pCMV-3×FLAG-SLC32A1-Neo plasmid

PVT92660

pCMV-3×FLAG-SLC32A1-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-SLC32A1(human)-Neo
Alias: NM_080552.3;DEE114; GEFSP12; VGAT; VIAAT; VIAAT,GEFSP12;
Gene lenght: 1578bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-SLC32A1-Neo plasmid Description

pCMV-3×FLAG-SLC32A1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-SLC32A1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggccacct tgctccgcag caagctgtcc 1021 aacgtggcca cgtccgtgtc caacaagtcc caggccaaga tgagcggcat gttcgccagg 1081 atgggttttc aggcggccac ggatgaggag gcggtgggct tcgcgcattg cgacgacctc 1141 gactttgagc accgccaggg cctgcagatg gacatcctga aagccgaggg agagccctgc 1201 ggggacgagg gcgctgaagc gcccgtcgag ggagacatcc attatcagcg aggcagcgga 1261 gctcctctgc cgccctccgg ctccaaggac caggtgggag gtggtggcga attcgggggc 1321 cacgacaagc ccaaaatcac ggcgtgggag gcaggctgga acgtgaccaa cgccatccag 1381 ggcatgttcg tgctgggcct accctacgcc atcctgcacg gcggctacct ggggttgttt 1441 ctcatcatct tcgccgccgt tgtgtgctgc tacaccggca agatcctcat cgcgtgcctg 1501 tacgaggaga atgaagacgg cgaggtggtg cgcgtgcggg actcgtacgt ggccatagcc 1561 aacgcctgct gcgccccgcg cttcccaacg ctgggcggcc gagtggtgaa cgtagcgcag 1621 atcatcgagc tggtgatgac gtgcatcctg tacgtggtgg tgagtggcaa cctcatgtac 1681 aacagcttcc cggggctgcc cgtgtcgcag aagtcctggt ccattatcgc cacggccgtg 1741 ctgctgcctt gcgccttcct taagaacctc aaggccgtgt ccaagttcag tctgctgtgc 1801 actctggccc acttcgtcat caatatcctg gtcatagcct actgtctatc gcgggcgcgc 1861 gactgggcct gggagaaggt caagttctac atcgacgtca agaagttccc catctccatt 1921 ggcatcatcg tgttcagcta cacgtctcag atcttcctgc cttcgctgga gggcaatatg 1981 cagcagccca gcgagttcca ctgcatgatg aactggacgc acatcgcagc ctgcgtgctc 2041 aagggcctct tcgcgctcgt cgcctacctc acctgggccg acgagaccaa ggaggtcatc 2101 acggataacc tgcccggctc catccgcgcc gtggtcaaca tctttctggt ggccaaggcg 2161 ctgttgtcct atcctctgcc attctttgcc gctgtcgagg tgctggagaa gtcgctcttc 2221 caggaaggca gccgcgcctt tttcccggcc tgctacagcg gcgacgggcg cctgaagtcc 2281 tgggggctga cgctgcgctg cgcgctcgtc gtcttcacgc tgctcatggc catttatgtg 2341 ccgcacttcg cgctgctcat gggcctcacc ggcagcctca cgggcgccgg cctctgtttc 2401 ttgctgccca gcctctttca cctgcgcctg ctctggcgca agctgctgtg gcaccaagtc 2461 ttcttcgacg tcgccatctt cgtcatcggc ggcatctgca gcgtgtccgg cttcgtgcac 2521 tccctcgagg gcctcatcga agcctaccga accaacgcgg aggactagga attctgcaga 2581 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc 2641 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 2701 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 2761 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 2821 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 2881 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 2941 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 3001 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 3061 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 3121 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 3181 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 3241 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 3301 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 3361 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3421 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 3481 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 3541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 3601 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 3661 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 3721 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 3781 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 3841 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 3901 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 3961 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 4021 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 4081 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 4141 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 4201 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 4261 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 4321 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 4381 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 4441 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 4501 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 4561 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 4621 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 4681 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 4741 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 4801 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 4861 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 4921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 4981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 5041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 5101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 5161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 5221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 5281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 5521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 5761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 5821 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5881 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5941 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6001 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 6061 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6121 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6181 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6241 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6301 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6361 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6421 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6481 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6541 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6601 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6661 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6721 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6781 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6841 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6901 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6961 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 7021 tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.