pTac-GST-USP53 plasmid

pTac-GST-USP53 plasmid

PVT92005

pTac-GST-USP53 plasmid Information

Plasmid name: pTac-GST-USP53(human)
Alias: NM_019050.3;PFIC7;
Gene lenght: 3222bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: AmpNR
Copy number: High
Induction: IPTG Induction

pTac-GST-USP53 plasmid Description

pTac-GST-USP53 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-USP53 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggc atgggtaaaa ttcttacgga 961 aacctggtgg caatcttgga aaagtttatc agcctggaag tatgctatca ctagccccta 1021 ccaaaggctt gttaaatgaa ccaggacaaa acagctgctt tcttaatagc gctgtacagg 1081 ttttatggca attggatata ttccgacgaa gcttgcgggt tttgactgga catgtttgtc 1141 agggagatgc ctgtatattt tgtgcattga agacgatatt tgcacagttc caacacagtc 1201 gagaaaaagc acttccctca gataacataa ggcatgctct tgcagaaagt ttcaaagatg 1261 agcagcgatt tcaacttggc cttatggatg atgctgcgga gtgctttgaa aatatgttgg 1321 agaggattca ttttcacata gtgccaagca gagatgcaga catgtgtacc tctaaatctt 1381 gtatcactca ccagaagttt gctatgactc tgtatgaaca gtgtgtgtgt cgtagctgtg 1441 gagcatcgtc agatcctcta ccttttacag aatttgtgcg gtacatttct acaacagcct 1501 tatgcaatga ggttgaaaga atgttggaaa ggcatgaacg ctttaaacct gaaatgtttg 1561 cagaattgct acaagcagca aatacaacag atgactatag gaaatgtcct agtaactgtg 1621 gccaaaaaat aaaaattcgc cgtgttttaa tgaattgccc agagattgtt acaattggtt 1681 tagtctggga ctccgagcat tctgacttga ccgaagctgt tgttcggaat ctagcaacac 1741 atctttatct tcctgggctt ttttatagag ttactgatga aaatgccaaa aatagtgaac 1801 ttaaccttgt tggtatgatc tgctacacca gccaacatta ttgtgccttt gcatttcaca 1861 ccaaaagttc caaatgggta ttttttgatg atgcaaatgt gaaagagatt ggaactagat 1921 ggaaagatgt tgtctccaaa tgcattcgat gccactttca gccactactt ttgttttatg 1981 caaacccaga tggcacagca gtttctactg aggatgcact caggcaggtc atcagctggt 2041 cacattacaa atctgttgca gaaaatatgg gatgtgaaaa gcctgtaatt cataagtcag 2101 ataatttaaa agaaaatgga tttggtgatc aggcaaagca gagagaaaat cagaaatttc 2161 caactgataa tatttcatca tctaatcgga gccacagtca cacaggtgta gggaaaggac 2221 cagctaagtt aagtcacatt gatcaaaggg aaaagataaa agacatttcc agagaatgtg 2281 ctctgaaagc tattgaacag aaaaacttac tttcttcaca aaggaaagat ttagagaagg 2341 gacaaagaaa agatttagga cgacatagag atttggttga tgaagacctt tcacatttcc 2401 aatctggatc acctcctgcc ccaaatggtt ttaaacaaca tgggaatcca catctatatc 2461 atagtcaagg aaaaggatca tataaacatg accgagttgt acctcagagt cgagcttctg 2521 cacaaataat aagttcaagt aaatcccaga ttcttgctcc aggagagaaa ataactggca 2581 aagttaagag tgacaatggc actggatatg acacagacag cagccaagat tctagggata 2641 gaggaaacag ctgtgatagc agcagtaaaa gccggaaccg aggttggaaa cctatgagag 2701 aaacattaaa tgttgatagt atttttagtg aaagtgaaaa aagacagcat agtccaagac 2761 ataaaccaaa tatcagtaat aagcctaaat ctagcaagga tccgagtttt agtaattggc 2821 caaaagagaa tccaaagcaa aaaggtttaa tgaccatata tgaagatgaa atgaagcagg 2881 aaataggaag cagaagttcc cttgaatcta atggaaaagg agcagagaaa aataaaggcc 2941 ttgtagaggg taaagtgcat ggtgataatt ggcagatgca aaggactgag tctggatatg 3001 aaagcagtga tcacatcagt aatggttcta ctaatttgga ctcacctgtt atcgatggaa 3061 atggtacagt aatggatatc agtggtgtta aagaaacagt atgcttcagt gaccagatta 3121 cgacaagcaa cctaaataaa gaacgtgggg actgtacctc ccttcagagc caacatcact 3181 tagaaggctt tagaaaagaa ctcaggaatt tggaagcagg ctataaatct catgaattcc 3241 acccagaatc acatttacaa ataaaaaatc atttgataaa aagatcacat gtacatgaag 3301 acaatggaaa gttatttcct tcatccagtc tacaaatacc caaggaccat aatgcaagag 3361 aacatatcca ccagtcagat gaacagaaac ttgaaaaacc gaatgaatgc aaattttctg 3421 agtggcttaa tatagaaaat tctgagagaa caggtttgcc ttttcacgtt gataactctg 3481 cttctgggaa gagagtgaac agtaatgaac catcttcatt atggtcttca cacctaagaa 3541 ctgttgggtt aaagccagaa actgctcctc tcatccagca acaaaatatc atggatcaat 3601 gttactttga gaactctcta tccacagaat gtataattcg gtcagccagc agatctgatg 3661 ggtgtcagat gccaaaactt ttttgccaga atctaccacc ccctttgcca ccaaagaaat 3721 atgctataac cagtgtgcca cagtcagaga aaagcgaatc tacacctgat gtcaaactta 3781 cagaggtgtt taaagctacc tctcatcttc cgaagcacag tttaagtaca gcttcagaac 3841 caagtttaga agtgagtaca catatgaatg atgaaagaca taaagaaaca tttcaagtga 3901 gagaatgttt tggcaacaca ccaaactgtc catccagctc ctcaactaac gattttcagg 3961 caaactcagg tgccattgat gcattttgcc aaccagaact agactctatt tctacctgtc 4021 caaatgagac agtttcatta actacctatt tttcagttga tagctgcatg acggatacat 4081 atagattgaa ataccatcag aggcccaagc tctcttttcc agagagcagt ggcttttgta 4141 ataattcact atcttagctc gagcggccgc atcgtgactg actgacgatc tgcctcgcgc 4201 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 4261 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 4321 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa 4381 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 4441 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 4501 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 4561 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 4621 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 4681 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 4741 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 4801 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 4861 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 4921 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 4981 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 5041 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 5101 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 5161 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 5221 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 5281 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 5341 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 5401 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 5461 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 5521 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 5581 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 5641 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 5701 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 5761 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 5821 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 5881 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 5941 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 6001 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 6061 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 6121 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 6181 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 6241 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 6301 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 6361 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 6421 gtatttcaca ccgcataaat tccgacacca tcgaatggtg caaaaccttt cgcggtatgg 6481 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat 6541 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg 6601 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt 6661 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg 6721 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg 6781 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct 6841 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc 6901 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat 6961 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta 7021 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg 7081 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc 7141 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc 7201 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 7261 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 7321 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 7381 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 7441 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 7501 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 7561 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 7621 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 7681 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 7741 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 7801 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 7861 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc 7921 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt 7981 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac 8041 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc 8101 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga 8161 tggcgttgga att

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.