pHIV-EF1a-Ugt2b5-IRES2-EGFP pl

pHIV-EF1a-Ugt2b5-IRES2-EGFP plasmid

PVT91769

pHIV-EF1a-Ugt2b5-IRES2-EGFP plasmid Information

Plasmid name: pHIV-EF1a-Ugt2b5(mouse)-IRES2-EGFP
Alias: NM_009467.3;M-1; Udpgt-3; Udpgt2b5; Ugt2b17;
Gene lenght: 1593bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pHIV-EF1a-Ugt2b5-IRES2-EGFP plasmid Description

pHIV-EF1a-Ugt2b5-IRES2-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pHIV-EF1a-Ugt2b5-IRES2-EGFP plasmid Sequence

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga 1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt 1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg 1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc 1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa 1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa 1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg 1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg 1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga 2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga 2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa 2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg 2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc 2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga 2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact 2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg 2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca 2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 3781 tcttccattt caggtgtcgt gagcggccgc tgagttaact attctagaGC CACCatgcct 3841 ggaaagtgga tttctgctct gctgctgctg cagataagtt gctgcttcag atctgtgaaa 3901 tgtgggaaag tgttggtgtg gccgatggaa ttcagtcatt ggatgaacat aaaaataata 3961 ctggatgaac tggtacagag aggccatgaa gtcactgttc tgagaccttc agcttactat 4021 gttcttgatc ccaaaaaatc acctggcctt aagtttgaaa cttttcctac atctgtcagt 4081 aaagacaatc tggaaaattt tttcattaag tttgtggatg tgtggactta tgaaatgcca 4141 agggatacat gtttgtcata ttctccttta ctgcaaaata tgattgatga attttctgat 4201 tattttctaa gtctttgtaa agatgttgtt tcaaacaaag agctcatgac aaagctacag 4261 gaatccaagt ttgatgtcct tttgtcagat cctgttgctt cctgtgggga gctcatagct 4321 gaactgctcc agattccttt tctgtacagt atccgcttct ctccaggcta ccaaattgaa 4381 aagtccagcg ggagatttct actccctccc tcttatgtac ctgtaattct gtcaggatta 4441 ggtggacaaa tgacattcat agagaggata aaaaatatga tatgcatgct ttattttgac 4501 ttttggttcc agatgtttaa tgataagaaa tgggactcat tttacagtga gtatttggga 4561 aggccaacca ccttagttga gacaatgggc caagcagaaa tgtggctcat tagatccaac 4621 tgggatttgg agtttcctca cccaacctta ccaaatgttg actatgttgg aggactccat 4681 tgcaaacctg ctaaaccctt gcctaaggat atggaagaat ttgtccagag ctctggagac 4741 catggtgtgg tggtcttttc tcttgggtca atggttagta acatgacaga agaaaaagcc 4801 aacgcaattg catgggccct tgcccagatt ccacaaaagg ttctttggaa atttgatggc 4861 aaaaccccag caactttagg acacaatacc agagtgtaca agtggctccc ccagaatgac 4921 ctccttggtc atccaaaaac caaagccttt gtaactcatg gtggggccaa tggtgtctac 4981 gaggcgatct atcatggaat ccctatgatt ggcattcctt tgtttggaga acagcatgat 5041 aacattgccc atatggtggc caaaggagca gctgttgcat tgaatatcag aacaatgtca 5101 aagtcagatg tgctcaatgc actggaggag gtcatagaaa atcctttcta taaaaagaat 5161 gctatgtggt tgtcaaccat tcaccatgac cagcctatga aacccctgga cagggctgtc 5221 ttctggattg agtttgttat gcgccacaaa agagccaagc acctgagacc acttggacat 5281 aaccttacct ggtaccagta ccactctctg gatgtgattg gattcctact ctcttgtgtg 5341 gcaaccacta tagtccttag tgtaaagtgc ctcttgttca tttaccgatt ctttgtaaag 5401 aaagaaaata aaatgaagaa tgagtaggga tccgcccctc tccctccccc ccccctaacg 5461 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 5521 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 5581 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 5641 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 5701 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 5761 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 5821 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 5881 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga 5941 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 6001 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg 6061 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 6121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 6181 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 6241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 6301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 6361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 6421 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 6481 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 6541 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 6601 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 6661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 6721 atggacgagc tgtacaagta aagcggccgc atcgataccg tcgacctcga tcgagaccta 6781 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 6841 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga 6901 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 6961 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca 7021 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg 7081 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat 7141 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag 7201 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag 7261 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 7321 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 7381 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7441 gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 7501 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 7561 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 7621 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 7681 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 7741 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7801 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7861 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 7921 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 7981 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 8041 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 8101 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 8161 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 8221 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8281 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8341 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8401 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8461 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8521 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8581 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 8641 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 8701 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 8761 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 8821 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 8881 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 8941 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 9001 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 9061 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 9121 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 9181 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 9241 ccgcgcacat ttccccgaaa agtgccacct gac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.