pLV3-CMV-ZDHHC13-3×HA-EF1a-mCh

pLV3-CMV-ZDHHC13-3×HA-EF1a-mCherry-Puro plasmid

PVT91701

pLV3-CMV-ZDHHC13-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-ZDHHC13(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_019028.3;HIP14L; HIP3RP
Gene lenght: 1866bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ZDHHC13-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-ZDHHC13-3×HA-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ZDHHC13-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggaggg gccggggctg ggctcgcagt gcaggaatca 2341 cagccatggc ccccaccctc caggatttgg tcgatatggc atctgtgcac atgaaaacaa 2401 agaacttgcc aatgcaagag aagctcttcc tcttatagag gactctagta actgtgacat 2461 tgtcaaagct actcaatacg gaatttttga acgatgtaaa gagttggtag aagcaggata 2521 tgatgtcagg caaccagata aagaaaatgt gtcgcttctt cattgggctg ctattaacaa 2581 cagactggat cttgtaaagt tttatatttc aaaaggtgct gttgtagatc agttgggtgg 2641 agatttaaat tcaactcctc ttcactgggc catccgacaa ggacatttac ctatggtcat 2701 attattactc cagcatggtg cagaccccac tcttattgat ggagagggat tcagcagcat 2761 ccacctggca gtattgtttc aacacatgcc tattatagca tatctcatct caaagggaca 2821 gagtgtgaat atgacagatg taaatgggca gacacctctc atgttatcag ctcacaaagt 2881 aattgggcca gaaccaactg gatttctttt aaagtttaat ccttctctca atgtggttga 2941 taaaatacac caaaacactc cacttcactg ggcagttgca gcaggaaatg ttaatgcagt 3001 tgataagctt ttggaagctg gttctagcct ggatatccag aatgttaagg gagaaacacc 3061 tcttgatatg gctctacaaa acaaaaatca gctcattatt catatgctaa aaacagaagc 3121 caaaatgaga gccaaccaaa agttcagact ttggaggtgg ctgcagaaat gcgagctctt 3181 cctgctgctg atgctttctg tgattaccat gtgggctatt ggatacatat tggacttcaa 3241 ttcagattct tggcttttaa aaggatgtct tctagtaaca ctgttttttc tgacatcttt 3301 gtttccaagg ttcttggttg ggtataagaa ccttgtatac ttaccaacag cctttctgct 3361 aagttctgtt ttttggatat ttatgacttg gttcatctta ttttttcctg atttagcagg 3421 agcccctttc tatttcagtt tcattttcag catagtagcc tttctatact ttttctataa 3481 gacttgggca actgatccag gcttcactaa ggcttctgaa gaagaaaaga aagtgaatat 3541 catcaccctt gcagaaactg gctctctgga cttcagaaca ttttgtacat catgtcttat 3601 aaggaagcca ttaaggtcac tccactgcca tgtatgcaac tgctgtgtgg ctcgatatga 3661 tcaacactgc ctgtggactg gacggtgcat aggttttggc aaccatcact attacatatt 3721 cttcttgttt ttcctttcca tggtatgtgg ctggattata tatggatctt tcatctattt 3781 gtccagtcat tgtgccacaa cattcaaaga agatggatta tggacttacc tcaatcagat 3841 tgtggcctgt tccccttggg ttttatatat cttgatgcta gcaactttcc atttctcatg 3901 gtcaacattt ttattattaa atcaactctt tcagattgcc tttctgggcc tgacctccca 3961 tgagagaatc agcctgcaga agcagagcaa gcatatgaaa cagacgttgt ccctcaggaa 4021 gacaccatac aatcttggat tcatgcagaa cctggcagat ttctttcagt gtggctgctt 4081 tggcttggtg aagccctgtg tggtagattg gacatcacag tacaccatgg tctttcaccc 4141 agccagggag aaggttcttc gctcagtagg atccTACCCA TACGACGTAC CTGATTATGC 4201 AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC 4261 ATAGgcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 4321 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 4381 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 4441 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 4501 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 4561 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 4621 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 4681 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 4741 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 4801 agctgtgacc ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca 4861 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 4921 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 4981 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 5041 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 5101 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 5161 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 5221 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 5281 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 5341 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 5401 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 5461 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 5521 gcatggacga gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg 5581 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc 5641 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 5701 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 5761 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 5821 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 5881 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 5941 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 6001 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 6061 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 6121 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 6181 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga 6241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 6301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 6361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 6421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 6481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 6541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 6601 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 6661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6721 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc 6781 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6841 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 6901 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 6961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 7021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 7081 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 7141 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 7201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 7261 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 7321 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 7381 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 7441 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 7501 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7561 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7621 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7801 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7861 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7981 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 8041 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 8101 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 8161 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 8221 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 8281 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8341 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 8401 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8461 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8521 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8581 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8641 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8701 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8761 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8821 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8881 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8941 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 9001 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 9061 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 9121 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 9181 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 9241 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9301 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9361 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9421 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9481 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9541 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9601 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9661 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9721 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9781 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9841 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9901 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9961 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 10021 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 10081 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.