pLV3-CMV-Tgfb1-3×FLAG-CopGFP-P

pLV3-CMV-Tgfb1-3×FLAG-CopGFP-Puro plasmid

PVT91682

pLV3-CMV-Tgfb1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Tgfb1(rat)-3×FLAG-CopGFP-Puro
Alias: NM_021578.2;Tgfb
Gene lenght: 1170bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Tgfb1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Tgfb1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Tgfb1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc cgccctcggg gctgcggctg 2341 ctgccgcttc tgctcccact cccgtggctt ctagtgctga cgcccgggag gccagccgcg 2401 ggactctcca cctgcaagac catcgacatg gagctggtga aacggaagcg catcgaagcc 2461 atccgtggcc agatcctgtc caaactaagg ctcgccagtc ccccgagcca gggggaggta 2521 ccgccgggcc cgctgcccga ggcggtgctc gctttgtaca acagcacccg cgaccgggtg 2581 gcaggcgaga gcgctgaccc ggagcccgag cccgaggcgg actactacgc caaagaagtc 2641 acccgcgtgc taatggtgga ccgcaacaac gcaatctatg acaaaaccaa agacatcaca 2701 cacagtatat atatgttctt caatacgtca gacattcggg aagcagtgcc agaaccccca 2761 ttgctgtccc gtgcagagct gcgcctgcag agattcaagt caactgtgga gcaacacgta 2821 gaactctacc agaaatatag caacaattcc tggcgttacc ttggtaaccg gctgctgacc 2881 cccactgata cgcctgagtg gctgtctttt gacgtcactg gagttgtccg gcagtggctg 2941 aaccaaggag acggaataca gggctttcgc ttcagtgctc actgctcttg tgacagcaaa 3001 gataatgtac tccacgtgga aatcaatggg atcagtccca aacgtcgagg tgacctgggc 3061 accatccatg acatgaaccg acccttcctg ctcctcatgg ccacccccct ggaaagggct 3121 caacacctgc acagctccag gcaccggaga gccctggata ccaactactg cttcagctcc 3181 acagagaaga actgctgtgt acggcagctg tacattgact ttaggaagga cctgggttgg 3241 aagtggatcc acgagcccaa gggctaccat gccaacttct gtctggggcc ctgcccctac 3301 atttggagcc tggacacaca gtacagcaag gtccttgccc tctacaacca acacaacccg 3361 ggtgcttccg catcaccgtg ctgcgtgccg caggctttgg agccactgcc catcgtctac 3421 tacgtgggtc gcaagcccaa ggtggagcag ttgtccaaca tgatcgtgcg ctcctgcaag 3481 tgcagcggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3541 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3601 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3661 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3721 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3781 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3841 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3901 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3961 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4021 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4081 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4141 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4201 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4261 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4321 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4381 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4441 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4501 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4561 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4621 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4681 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4741 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4801 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4861 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4921 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4981 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5041 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5101 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5161 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5221 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5281 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5341 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5401 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5461 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5521 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5581 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5641 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5701 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5761 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5821 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5881 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 5941 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6001 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6061 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6121 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6181 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6241 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6421 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6481 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6601 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6661 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6721 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6781 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6841 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6901 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6961 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7861 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8281 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8581 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9001 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9181 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9241 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9301 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9361 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9421 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.