pCMV-Nfatc2-2-3×FLAG-Neo plasm

pCMV-Nfatc2-2-3×FLAG-Neo plasmid

PVT91386

pCMV-Nfatc2-2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Nfatc2(mouse)-2-3×FLAG-Neo
Alias: NM_010899.3;NF-ATc2; NF-ATp; NFAT1; NFAT1-D; Nfatp;
Gene lenght: 2781bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Nfatc2-2-3×FLAG-Neo plasmid Description

pCMV-Nfatc2-2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Nfatc2-2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga cgtcccggag ccgcagcccg 781 accccgatgg cggggacggc cccggccacg agcccggggg cagtccccaa gacgagctgg 841 acttttccat cctcttcgat tatgactatc tgaaccctat cgaagaagaa ccgatcgcac 901 ataaggccat cagctcaccc tccggactcg catacccgga tgatgtcctg gactatggcc 961 tcaagccatg caaccccctt gccagtctct ctggcgagcc ccctggccgg ttcggagagc 1021 cggatagtat agggttccag aactttctga gcccggtcaa gccagcaggg gcttcgggcc 1081 cgagccctcg gatcgagatc actccatccc acgaactgat gcaggcaggg ggggccctcc 1141 gtgggagaga cgccggcctg tcccccgagc agccggccct ggccctggcc ggcgtggccg 1201 ccagcccgag gttcacactg cccgtgcccg gctacgaggg ctaccgcgag ccgctttgct 1261 tgagccccgc tagcagcggc tcctctgcca gcttcatttc tgacaccttc tccccctaca 1321 cctcgccctg cgtctcaccc aataacgccg ggcccgacga cctgtgtccc cagtttcaaa 1381 acatccctgc tcattattcc cccagaacct ctccaataat gtcacctcga accagcctcg 1441 ccgaggacag ctgcctgggc cgacactcgc ccgtgccccg tccggcatcc cgctcctcct 1501 cacccggtgc caagcggagg cattcgtgcg cagaggcttt ggttgctcct ctgcccgcag 1561 cctcacccca gcgctcccgg agcccctcgc cacagccctc gcctcacgtg gcactgcagg 1621 acgacagcat ccccgctggg taccccccca cggccggctc tgctgttctc atggatgccc 1681 tcaacaccct ggccaccgac tcgccctgcg ggatcccctc caagatatgg aagaccagtc 1741 ctgacccgac gcctgtgtcc accgctccgt ccaaggctgg cctggcccgc cacatctacc 1801 ctactgtgga gttcctgggg ccatgtgagc aggaggagag gaggaattcc gctccagagt 1861 ccatcctgct ggtaccacct acttggccca agcagttggt gccggccatt cccatctgca 1921 gcatccctgt gactgcatcc ctcccaccac tcgagtggcc actctccaat cagtcgggct 1981 cctatgagct acggattgag gtccaaccca agccccatca ccgggcccac tatgagacgg 2041 agggcagccg tggcgctgtc aaagccccaa caggaggaca ccctgtggtg cagctccacg 2101 gctacatgga gaacaagcct ctggggcttc agatcttcat tgggacagca gatgagagga 2161 tccttaagcc gcacgccttc taccaagtac acaggatcac tgggaaaacg gtcaccacca 2221 cgagctatga gaagatcgta ggcaacacca aggtcctgga gatccccctg gagccaaaga 2281 acaacatgag agccaccatc gactgtgcag gcatcctgaa gctccgaaac gctgacatcg 2341 agctgcggaa gggcgagacg gacatcggca ggaagaacac gcgtgtgcgc ctggtgttcc 2401 gcgtgcacgt cccagagccc agtgggcgca tcgtctccct gcaggctgcg tccaacccca 2461 tcgagtgctc tcagcgctct gcccacgagc tgcccatggt ggagagacaa gacatggaca 2521 gctgcctggt ctacgggggc cagcagatga tcctcacggg ccagaacttc acagcggagt 2581 ccaaggttgt gttcatggag aagactacag atgggcagca gatttgggag atggaagcta 2641 cggtggataa agacaagagc cagcctaaca tgctttttgt tgagatcccc gagtatcgga 2701 acaagcacat ccgcgtgccc gtgaaagtca acttctacgt catcaacgga aagaggaaac 2761 gaagtcagcc acagcacttt acctaccacc cagtccctgc catcaagaca gagcccagcg 2821 atgagtatga accatctttg atctgcagcc ccgcccatgg aggcctgggg agccagccat 2881 attacccaca gcacccaatg ctggccgagt ccccctcctg ccttgtggct accatggccc 2941 cctgccaaca gttccgctcg gggctctcat cccccgatgc tcgctaccaa cagcagagcc 3001 ccgcagctgc cctctaccag agaagcaaga gcctgagtcc cggcctgctg ggctaccagc 3061 agccgtccct cctggcagca cccttgggtc tggctgatgc ccaccgctct gtgctggtgc 3121 atgctggttc tcaggggcag gggcagggct ccaccctgcc acacacatcc tcggccagcc 3181 agcaggcctc acccgtgatc cactactcac ccaccaacca gcagcttcgc ggtgggggtc 3241 accaggagtt ccagcatatc atgtactgtg aaaacttcgg ccccagctct gccaggcctg 3301 gcccgcctcc catcaaccaa ggtcagaggc tgagcccggg cgcctacccc acagtcatcc 3361 aacaacagac tgccccgagc caaagagctg ccaaaaacgg acccagtgac cagaaggaag 3421 ctctgcccac gggagtgacc gtcaaacagg aacagaacct ggaccagacc tacctggatg 3481 acgttaatga aatcatcagg aaggagtttt caggacctcc ctcccgaaat cagaccGGAT 3541 CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 3601 ATGACAAGTA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 3661 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 3721 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 3781 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 3841 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 3901 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 3961 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 4021 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 4081 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 4141 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 4201 CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA 4261 ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT 4321 TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC 4381 ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC 4441 AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC 4501 AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 4561 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4621 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4681 TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4741 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 4801 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 4861 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA 4921 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 4981 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5041 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 5161 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 5221 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 5281 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 5341 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 5401 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 5461 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 5521 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 5581 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 5641 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 5701 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 5761 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 5821 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 5881 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 5941 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 6001 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 6061 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 6121 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 6181 GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 6241 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 6301 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 6361 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 6421 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 6481 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 6541 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 6601 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 6661 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 6721 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 6781 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 6841 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 6901 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 6961 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 7021 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 7081 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT 7141 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 7201 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 7261 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 7321 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 7381 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 7441 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 7501 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 7561 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 7621 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 7681 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 7741 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 7801 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 7861 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 7921 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 7981 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 8041 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 8101 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 8161 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 8221 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 8281 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 8341 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 8401 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 8461 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 8521 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 8581 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 8641 CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA 8701 TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT 8761 GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA 8821 GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.