pHW2000-HA(H14N6) plasmid

pHW2000-HA(H14N6) plasmid

PVT90930

pHW2000-HA(H14N6) plasmid Information

Plasmid name: pHW2000-HA(H14N6)
Alias: -
Gene lenght: 1707bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: influenza virus
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: PolI
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HA
Reverse primer:
Copy number: High
Induction:

pHW2000-HA(H14N6) plasmid Description

pHW2000-HA(H14N6) is a plasmid suitable for protein expression in mammalian cells.

pHW2000-HA(H14N6) plasmid Sequence

1 cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct 61 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt 121 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat 181 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg 241 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg 301 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac 361 gtcaataggg ggcgtacttg gcatatcgac gtcaggtggc acttttcggg gaaatgtgcg 421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 1021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 1081 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 1141 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 1201 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 1261 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 1321 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 1381 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 1441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 1501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 1561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 1621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 1681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 1741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 1801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 1861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 1921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 1981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 2041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 2101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 2161 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 2221 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 2281 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 2341 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggttctttc cgcctcagaa 2401 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg 2461 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca 2521 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc 2581 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag 2641 cgagctctag catttaggtg accgccggga gggcgtcccc ggcccggcgc tgctcccgcg 2701 tgtgtcctgg ggttgaccag agggccccgg gcgctccgtg tgtggctgcg atggtggcgt 2761 ttttggggac aggtgtccgt gtcgcgcgtc gcctgggccg gcggcgtggt cggtgacgcg 2821 acctcccggc cccgggggag gtatatcttt cgctccgagt cggcattttg ggccgccggg 2881 ttattggaga cggtaccagc aaaagcaggg gaaaatgatt gcattcatag tgattgcact 2941 ggctctaagc cacactactt actcccagat cacaaatgga accacaggaa atcctgttat 3001 atgtttgggc caccatgcag tggaaaatgg cacatctgtc aaaacactga cagacaatca 3061 tgtggaggtt gtatcggcta gagaattggt tgagacaaac catactgacg aactatgtcc 3121 gagccctctg aagctcgtcg atgggcaaga ctgcgacctc atcaatggtg cattagggag 3181 tccaggctgt gaccacttac aagacactac ttgggatgtt ttcattgaaa ggccgactgc 3241 agtagacaca tgttatccat tcgatgttcc tgattaccag agtcttagaa gcatcttagc 3301 aagcagtggg agtctggagt tcattgccga acaattcact tggaatggcg tcaaagttga 3361 cggatcaagc agtgcttgtt taaggggagg ccgtaatggc ttcttctccc gactgaattg 3421 gctaaccaaa gcaacagatg ggaactatag gcccattaat gttacaaaag aaaacacggg 3481 atcttatgtt aggctctacc tctggggagt gcatcatcca tcaagtgata aagagcaaac 3541 ggatctttac aaagttgcaa caggaagagt gacggtgtct actcgctcgg atcagaccag 3601 tattgttcct aatataggga gtagaccaag agtgaggaac cagagcggca gaataagcat 3661 ctactggact ctagtaaacc caggggattc catcattttt aatagtattg gaaacttaat 3721 tgcaccaagg ggccactaca aaataggtaa acctacaaag ggcactgtgc ttcgaagtga 3781 caaaaagatt gggtcatgca caagcccttg cttgaccgat aaaggttcga tccaaagtga 3841 caaacctttt cagaatgtat ccaggatcgc cataggaaac tgcccgaagt atgtgaaaca 3901 ggggtcactg atgttggcaa ctggaatgcg caacatcccc gacaagcagg caaagggctt 3961 atttggagca attgctggtt tcattgaaaa tggttggcaa ggcttggttg atgggtggta 4021 cgggttcagg caccaaaatg ctgagggaac aggaactgct gcagatttga aatcgactca 4081 ggcagccctt gaccaaataa atggcaagct gaacagatta atagagaaga caaacgaaaa 4141 atatcatcag atcgaaaaag agtttgaaca agtcgaagga agaatacaag accttgagaa 4201 atatgtagag gacactaaaa ttgacttgtg gtcatacaat gccgaactat tagtagcctt 4261 ggaaaatcag cacacgatag atgttacaga ctctgagatg aataaactct ttgaaagggt 4321 gagaagacaa ctgagagaaa atgcagagga tcaaggcaac ggctgtttcg agatattcca 4381 tcaatgtgac aataactgta tagaaagcat tagaaatgga acctatgatc acaacatcta 4441 cagggatgaa gccatcaaca atcggattaa gataaatccc gtgaatttga caatgggcta 4501 caaggacgta atcctgtgga tttctttctc catgtcatgc tttgtcttcg tggcactgat 4561 cttggggttt gttctttggg cttgtcagaa cggaaacatc cgatgccaaa tttgtatata 4621 aagaaaaaac acccttgttt ctactgtctc ctccccccca acttcggagg tcgaccagta 4681 ctccggttaa ctgctagcgt taacagcttg ggtctcccta tagtgagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.