pCMV-UBA7-Neo plasmid

pCMV-UBA7-Neo plasmid

PVT90683

pCMV-UBA7-Neo plasmid Information

Plasmid name: pCMV-UBA7(human)-Neo
Alias: NM_003335.3;D8; UBA1B; UBE1L; UBE2; UBE7
Gene lenght: 3039bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-UBA7-Neo plasmid Description

pCMV-UBA7-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-UBA7-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gatgccctgg acgcttcgaa gctactggat gaggagctgt attcaagaca 961 gctgtatgtg ctgggctcac ctgccatgca gaggattcag ggagccaggg tcctggtgtc 1021 aggcctgcag ggcctggggg ccgaggtggc caagaacttg gttctgatgg gtgtgggcag 1081 cctcactctg catgatcccc accccacctg ctggtccgac ctggctgccc agtttctcct 1141 ctcagagcag gacttggaaa ggagcagagc cgaggcctct caagagctct tggctcagct 1201 caacagagct gtccaggtcg tcgtgcacac gggtgacatc actgaggacc tgctgttgga 1261 cttccaggtg gtggtgctga ctgctgcaaa gctggaggag cagctgaagg tgggcacctt 1321 gtgtcataag catggagttt gctttctggc ggctgacacc cggggcctcg tggggcagtt 1381 gttctgtgac tttggtgagg acttcactgt gcaggacccc acagaggcag aacccctgac 1441 agctgccatc cagcacatct cccagggctc ccctggcatt ctcactctga ggaaaggggc 1501 caatacccac tacttccgtg atggagactt ggtgactttc tcgggaattg agggaatggt 1561 tgagctcaac gactgtgatc cccggtctat ccacgtgcgg gaggatgggt ccctggagat 1621 tggagacaca acaactttct ctcggtactt gcgtggtggg gctatcactg aagtcaagag 1681 acccaagact gtgagacata agtccctgga cacagccctg ctccagcccc atgtggtggc 1741 ccagagctcc caggaagttc accatgccca ctgcctgcat caggccttct gtgcactgca 1801 caagttccag cacctccatg gccggccacc ccagccctgg gatcctgttg atgcagagac 1861 tgtggtgggc ctggcccggg acctggaacc actgaagcgg acagaggaag agccactgga 1921 agagccactg gatgaggccc tagtgcggac agtcgcccta agcagtgcag gtgtcttgag 1981 ccctatggtg gccatgctgg gtgcagtagc tgcccaggaa gtgctgaagg caatctccag 2041 gaagttcatg cctctggacc agtggcttta ctttgatgcc ctcgattgtc ttccggaaga 2101 tggggagctc cttcccagtc ctgaggactg tgccctgaga ggcagccgct atgatgggca 2161 aattgcagtg tttggggctg gttttcagga gaaactgaga cgccagcact acctcctggt 2221 gggcgctggt gccattggtt gtgagctgct caaagtcttt gccctagtgg gactgggggc 2281 cgggaacagc gggggcttga ctgttgttga catggaccac atagagcgct ccaatctcag 2341 ccgtcagttc ctcttcaggt cccaggacgt tggtagaccc aaggcagagg tggctgcagc 2401 agctgcccgg ggcctgaacc cagacttaca ggtgatcccg ctcacctacc cactggatcc 2461 caccacagag cacatctatg gggataactt tttctcccgt gtggatggtg tggctgctgc 2521 cctggacagt ttccaggccc ggcgctatgt ggctgctcgt tgcacccact atctgaagcc 2581 actgctggag gcaggcacat cgggcacctg gggcagtgct acagtattca tgccacatgt 2641 gactgaggcc tacagagccc ctgcctcagc tgcagcttct gaggatgccc cctaccctgt 2701 ctgtaccgtg cggtacttcc ctagcacagc cgagcacacc ctgcagtggg cccggcatga 2761 gtttgaagaa ctcttccgac tgtctgcaga gaccatcaac caccaccaac aggcacacac 2821 ttccctggca gacatggatg agccacagac actcacctta ctgaagccag tgcttggggt 2881 cctgagagtg cgtccacaga actggcaaga ctgtgtggcg tgggctcttg gccactggaa 2941 actctgcttt cattatggca tcaaacagct gctgaggcac ttcccaccta ataaagtgct 3001 tgaggatgga actcccttct ggtcaggtcc caaacagtgt ccccagccct tggagtttga 3061 caccaaccaa gacacacacc tcctctacgt actggcagct gccaacctgt atgcccagat 3121 gcatgggctg cctggctcac aggactggac tgcactcagg gagctgctga agctgctgcc 3181 acagcctgac ccccaacaga tggcccccat ctttgctagt aatctagagc tggcttcggc 3241 ttctgctgag tttggccctg agcagcagaa ggaactgaac aaagccctgg aagtctggag 3301 tgtgggccct cccctgaagc ctctgatgtt tgagaaggat gatgacagca acttccatgt 3361 ggactttgtg gtagcggcag ctagcctgag atgtcagaac tacgggattc caccggtcaa 3421 ccgtgcccag agcaagcgaa ttgtgggcca gattatccca gccattgcca ccactacagc 3481 agctgtggca ggcctgttgg gcctggagct gtataaggtg gtgagtgggc cacggcctcg 3541 tagtgccttt cgccacagct acctacatct ggctgaaaac tacctcatcc gctatatgcc 3601 ttttgcccca gccatccaga cgttccatca cctgaagtgg acctcttggg accgtctgaa 3661 ggtaccagct gggcagcctg agaggaccct ggagtcgctg ctggctcatc ttcaggagca 3721 gcacgggttg agggtgagga tcctgctgca cggctcagcc ctgctctatg cggccggatg 3781 gtcacctgaa aagcaggccc agcacctgcc cctcagggtg acagaactgg ttcagcagct 3841 gacaggccag gcacctgctc ctgggcagcg ggtgttggtg ctagagctga gctgtgaggg 3901 tgacgacgag gacactgcct tcccacctct gcactatgag ctgtgaCTCG AGTCTAGAGG 3961 GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 4021 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 4081 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 4141 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 4201 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA 4261 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC 4321 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC 4381 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG 4441 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC 4501 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG 4561 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 4621 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 4681 CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 4741 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 4801 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 4861 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 4921 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG 4981 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 5041 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 5101 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 5161 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 5221 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 5281 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 5341 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 5401 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 5461 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 5521 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 5581 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 5641 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 5701 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 5761 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 5821 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 5881 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 5941 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 6001 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 6061 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 6121 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 6181 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 6241 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 6301 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 6361 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 6421 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 6481 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 6541 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 6601 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 6661 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 6721 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 6781 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 6841 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 6901 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 6961 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 7021 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 7081 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 7141 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 7201 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 7261 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 7321 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 7381 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 7441 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 7501 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 7561 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 7621 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 7681 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 7741 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 7801 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 7861 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 7921 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 7981 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 8041 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 8101 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 8161 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 8221 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 8281 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 8341 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG 8401 GAGATCTCCC GATCCCCTAT GGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.