pLV3-U6-Colec11-sgRNA1-Cas9-EG

pLV3-U6-Colec11-sgRNA1-Cas9-EGFP-Puro plasmid

PVT90673

pLV3-U6-Colec11-sgRNA1-Cas9-EGFP-Puro plasmid Information

Plasmid name: pLV3-U6-Colec11(mouse)-sgRNA1-Cas9-EGFP-Puro
Alias: ccaggaagagtcggccctac;
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Colec11-sgRNA1-Cas9-EGFP-Puro plasmid Description

pLV3-U6-Colec11-sgRNA1-Cas9-EGFP-Puro This is a plasmid suitable for gene editing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-Colec11-sgRNA1-Cas9-EGFP-Puro plasmid Sequence

1 GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG 61 ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA 121 AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT 181 ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 241 CGAAACACCG Gccaggaaga gtcggcccta cGTTTTAGAG CTAGAAATAG CAAGTTAAAA 301 TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTGAATTCG 361 CTAGCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA 421 CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGATCC GGTGCCTAGA 481 GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG 541 AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG 601 GGTTTGCCGC CAGAACACAG GACCGGTTCT AGAGCGCTGC CACCATGGAC AAGAAGTACA 661 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA 721 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA 781 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA 841 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT 901 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC 961 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG 1021 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA 1081 CCGACAAGGC CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG 1141 GCCACTTCCT GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA 1201 TCCAGCTGGT GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG 1261 TGGACGCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GAAAATCTGA 1321 TCGCCCAGCT GCCCGGCGAG AAGAAGAATG GCCTGTTCGG AAACCTGATT GCCCTGAGCC 1381 TGGGCCTGAC CCCCAACTTC AAGAGCAACT TCGACCTGGC CGAGGATGCC AAACTGCAGC 1441 TGAGCAAGGA CACCTACGAC GACGACCTGG ACAACCTGCT GGCCCAGATC GGCGACCAGT 1501 ACGCCGACCT GTTTCTGGCC GCCAAGAACC TGTCCGACGC CATCCTGCTG AGCGACATCC 1561 TGAGAGTGAA CACCGAGATC ACCAAGGCCC CCCTGAGCGC CTCTATGATC AAGAGATACG 1621 ACGAGCACCA CCAGGACCTG ACCCTGCTGA AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA 1681 AGTACAAAGA GATTTTCTTC GACCAGAGCA AGAACGGCTA CGCCGGCTAC ATTGACGGCG 1741 GAGCCAGCCA GGAAGAGTTC TACAAGTTCA TCAAGCCCAT CCTGGAAAAG ATGGACGGCA 1801 CCGAGGAACT GCTCGTGAAG CTGAACAGAG AGGACCTGCT GCGGAAGCAG CGGACCTTCG 1861 ACAACGGCAG CATCCCCCAC CAGATCCACC TGGGAGAGCT GCACGCCATT CTGCGGCGGC 1921 AGGAAGATTT TTACCCATTC CTGAAGGACA ACCGGGAAAA GATCGAGAAG ATCCTGACCT 1981 TCCGCATCCC CTACTACGTG GGCCCTCTGG CCAGGGGAAA CAGCAGATTC GCCTGGATGA 2041 CCAGAAAGAG CGAGGAAACC ATCACCCCCT GGAACTTCGA GGAAGTGGTG GACAAGGGCG 2101 CTTCCGCCCA GAGCTTCATC GAGCGGATGA CCAACTTCGA TAAGAACCTG CCCAACGAGA 2161 AGGTGCTGCC CAAGCACAGC CTGCTGTACG AGTACTTCAC CGTGTATAAC GAGCTGACCA 2221 AAGTGAAATA CGTGACCGAG GGAATGAGAA AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA 2281 AGGCCATCGT GGACCTGCTG TTCAAGACCA ACCGGAAAGT GACCGTGAAG CAGCTGAAAG 2341 AGGACTACTT CAAGAAAATC GAGTGCTTCG ACTCCGTGGA AATCTCCGGC GTGGAAGATC 2401 GGTTCAACGC CTCCCTGGGC ACATACCACG ATCTGCTGAA AATTATCAAG GACAAGGACT 2461 TCCTGGACAA TGAGGAAAAC GAGGACATTC TGGAAGATAT CGTGCTGACC CTGACACTGT 2521 TTGAGGACAG AGAGATGATC GAGGAACGGC TGAAAACCTA TGCCCACCTG TTCGACGACA 2581 AAGTGATGAA GCAGCTGAAG CGGCGGAGAT ACACCGGCTG GGGCAGGCTG AGCCGGAAGC 2641 TGATCAACGG CATCCGGGAC AAGCAGTCCG GCAAGACAAT CCTGGATTTC CTGAAGTCCG 2701 ACGGCTTCGC CAACAGAAAC TTCATGCAGC TGATCCACGA CGACAGCCTG ACCTTTAAAG 2761 AGGACATCCA GAAAGCCCAG GTGTCCGGCC AGGGCGATAG CCTGCACGAG CACATTGCCA 2821 ATCTGGCCGG CAGCCCCGCC ATTAAGAAGG GCATCCTGCA GACAGTGAAG GTGGTGGACG 2881 AGCTCGTGAA AGTGATGGGC CGGCACAAGC CCGAGAACAT CGTGATCGAA ATGGCCAGAG 2941 AGAACCAGAC CACCCAGAAG GGACAGAAGA ACAGCCGCGA GAGAATGAAG CGGATCGAAG 3001 AGGGCATCAA AGAGCTGGGC AGCCAGATCC TGAAAGAACA CCCCGTGGAA AACACCCAGC 3061 TGCAGAACGA GAAGCTGTAC CTGTACTACC TGCAGAATGG GCGGGATATG TACGTGGACC 3121 AGGAACTGGA CATCAACCGG CTGTCCGACT ACGATGTGGA CCATATCGTG CCTCAGAGCT 3181 TTCTGAAGGA CGACTCCATC GACAACAAGG TGCTGACCAG AAGCGACAAG AACCGGGGCA 3241 AGAGCGACAA CGTGCCCTCC GAAGAGGTCG TGAAGAAGAT GAAGAACTAC TGGCGGCAGC 3301 TGCTGAACGC CAAGCTGATT ACCCAGAGAA AGTTCGACAA TCTGACCAAG GCCGAGAGAG 3361 GCGGCCTGAG CGAACTGGAT AAGGCCGGCT TCATCAAGAG ACAGCTGGTG GAAACCCGGC 3421 AGATCACAAA GCACGTGGCA CAGATCCTGG ACTCCCGGAT GAACACTAAG TACGACGAGA 3481 ATGACAAGCT GATCCGGGAA GTGAAAGTGA TCACCCTGAA GTCCAAGCTG GTGTCCGATT 3541 TCCGGAAGGA TTTCCAGTTT TACAAAGTGC GCGAGATCAA CAACTACCAC CACGCCCACG 3601 ACGCCTACCT GAACGCCGTC GTGGGAACCG CCCTGATCAA AAAGTACCCT AAGCTGGAAA 3661 GCGAGTTCGT GTACGGCGAC TACAAGGTGT ACGACGTGCG GAAGATGATC GCCAAGAGCG 3721 AGCAGGAAAT CGGCAAGGCT ACCGCCAAGT ACTTCTTCTA CAGCAACATC ATGAACTTTT 3781 TCAAGACCGA GATTACCCTG GCCAACGGCG AGATCCGGAA GCGGCCTCTG ATCGAGACAA 3841 ACGGCGAAAC CGGGGAGATC GTGTGGGATA AGGGCCGGGA TTTTGCCACC GTGCGGAAAG 3901 TGCTGAGCAT GCCCCAAGTG AATATCGTGA AAAAGACCGA GGTGCAGACA GGCGGCTTCA 3961 GCAAAGAGTC TATCCTGCCC AAGAGGAACA GCGATAAGCT GATCGCCAGA AAGAAGGACT 4021 GGGACCCTAA GAAGTACGGC GGCTTCGACA GCCCCACCGT GGCCTATTCT GTGCTGGTGG 4081 TGGCCAAAGT GGAAAAGGGC AAGTCCAAGA AACTGAAGAG TGTGAAAGAG CTGCTGGGGA 4141 TCACCATCAT GGAAAGAAGC AGCTTCGAGA AGAATCCCAT CGACTTTCTG GAAGCCAAGG 4201 GCTACAAAGA AGTGAAAAAG GACCTGATCA TCAAGCTGCC TAAGTACTCC CTGTTCGAGC 4261 TGGAAAACGG CCGGAAGAGA ATGCTGGCCT CTGCCGGCGA ACTGCAGAAG GGAAACGAAC 4321 TGGCCCTGCC CTCCAAATAT GTGAACTTCC TGTACCTGGC CAGCCACTAT GAGAAGCTGA 4381 AGGGCTCCCC CGAGGATAAT GAGCAGAAAC AGCTGTTTGT GGAACAGCAC AAGCACTACC 4441 TGGACGAGAT CATCGAGCAG ATCAGCGAGT TCTCCAAGAG AGTGATCCTG GCCGACGCTA 4501 ATCTGGACAA AGTGCTGTCC GCCTACAACA AGCACCGGGA TAAGCCCATC AGAGAGCAGG 4561 CCGAGAATAT CATCCACCTG TTTACCCTGA CCAATCTGGG AGCCCCTGCC GCCTTCAAGT 4621 ACTTTGACAC CACCATCGAC CGGAAGAGGT ACACCAGCAC CAAAGAGGTG CTGGACGCCA 4681 CCCTGATCCA CCAGAGCATC ACCGGCCTGT ACGAGACACG GATCGACCTG TCTCAGCTGG 4741 GAGGCGACAA GCGACCTGCC GCCACAAAGA AGGCTGGACA GGCTAAGAAG AAGAAAGATT 4801 ACAAAGACGA TGACGATAAG GGATCCGGCG CAACAAACTT CTCTCTGCTG AAACAAGCCG 4861 GAGATGTCGA AGAGAATCCT GGACCGATGG TGTCTAAGGG CGAAGAGCTG TTCACCGGGG 4921 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 4981 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 5041 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 5101 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 5161 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 5221 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 5281 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 5341 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 5401 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 5461 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 5521 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 5581 TCGGCATGGA CGAGCTGTAC AAGgagggca gaggaagtct tctaacatgc ggtgacgtgg 5641 aggagaatcc cggccctATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG 5701 ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC 5761 ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA 5821 CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG 5881 TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA 5941 TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC 6001 CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGAGTCTCG CCCGACCACC 6061 AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG 6121 GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG 6181 GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC 6241 GCAAGCCCGG TGCCTGActc gagTACGCGT TAAGTCGACA ATCAACCTCT GGATTACAAA 6301 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 6361 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 6421 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 6481 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 6541 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 6601 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 6661 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 6721 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 6781 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 6841 CGGATCTCCC TTTGGGCCGC CTCCCCGCGT CGACTTTAAG ACCAATGACT TACAAGGCAG 6901 CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC 6961 AACGAAGACA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG 7021 CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT 7081 GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA 7141 GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGGGCCCG TTTAAACCCG CTGATCAGCC 7201 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 7261 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 7321 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG 7381 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG 7441 GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC 7501 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 7561 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 7621 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 7681 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 7741 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 7801 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 7861 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATCACG 7921 TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 7981 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 8041 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 8101 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 8161 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 8221 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 8281 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 8341 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 8401 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 8461 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 8521 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 8581 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 8641 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 8701 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 8761 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 8821 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 8881 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 8941 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 9001 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 9061 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 9121 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 9181 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 9241 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 9301 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 9361 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 9421 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 9481 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 9541 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 9601 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 9661 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 9721 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 9781 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 9841 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 9901 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 9961 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 10021 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 10081 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 10141 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 10201 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 10261 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 10321 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT 10381 CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA 10441 GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA 10501 AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGGGT 10561 TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC ATTGATTATT 10621 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT 10681 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC 10741 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG 10801 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT 10861 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 10921 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT 10981 TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG 11041 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA 11101 ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG 11161 TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG 11221 ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT 11281 AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT 11341 AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG 11401 GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA 11461 AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG 11521 CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG 11581 ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA 11641 TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC 11701 ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA 11761 AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA 11821 GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC 11881 CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT 11941 GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA 12001 CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT 12061 TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG 12121 TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA 12181 TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA 12241 GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT 12301 CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC 12361 TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA 12421 CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG 12481 AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC 12541 TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT 12601 TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA 12661 CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG 12721 AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT GCGCCAATTC 12781 TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC 12841 AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA AGAATTACAA 12901 AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG AGATCCAGTT 12961 TGGTTAATTA AGGTACC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.