pTac-GST-TOP1 plasmid

pTac-GST-TOP1 plasmid

PVT90661

pTac-GST-TOP1 plasmid Information

Plasmid name: pTac-GST-TOP1(human-Toxic-opt)
Alias: NM_003286.4；TOPI；
Gene lenght: 2295bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pGEX-3'
Copy number: High
Induction: IPTG Induction

pTac-GST-TOP1 plasmid Description

pTac-GST-TOP1 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-TOP1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccATGAG CGGCGACCAT TTGCACAATG 961 ACAGCCAGAT TGAAGCAGAC TTTCGACTCA ACGACTCCCA TAAACACAAG GATAAGCACA 1021 AGGATAGAGA GCACAGACAC AAAGAGCACA AGAAAGAGAA GGACAGAGAA AAGTCCAAGC 1081 ACAGCAACTC TGAGCATAAA GACTCCGAAA AAAAGCATAA AGAGAAAGAG AAGACCAAAC 1141 ATAAAGACGG GAGCAGTGAG AAACACAAAG ACAAGCACAA GGACCGGGAT AAAGAAAAGC 1201 GAAAAGAGGA AAAAGTGAGA GCGAGTGGCG ACGCCAAAAT CAAGAAAGAG AAAGAAAACG 1261 GCTTTAGCTC ACCTCCCCAG ATTAAGGACG AGCCAGAAGA TGATGGATAC TTCGTACCGC 1321 CAAAGGAAGA CATCAAGCCC CTCAAACGCC CTAGGGATGA GGATGATGCC GACTACAAGC 1381 CCAAGAAAAT AAAGACCGAG GATACGAAAA AAGAAAAAAA GAGGAAACTT GAGGAAGAGG 1441 AAGACGGCAA GTTGAAAAAG CCTAAGAATA AGGACAAAGA CAAGAAAGTG CCTGAGCCGG 1501 ACAACAAGAA AAAGAAACCT AAGAAAGAGG AAGAACAGAA GTGGAAATGG TGGGAAGAGG 1561 AGCGCTACCC TGAAGGCATC AAGTGGAAAT TCCTCGAGCA TAAGGGACCG GTGTTTGCTC 1621 CACCTTACGA ACCTCTTCCA GAAAATGTGA AATTCTACTA TGATGGTAAG GTAATGAAGC 1681 TCTCCCCAAA GGCTGAGGAA GTAGCAACTT TCTTCGCCAA GATGTTGGAT CATGAGTACA 1741 CCACCAAGGA AATCTTTCGG AAGAACTTTT TCAAAGATTG GAGGAAAGAG ATGACTAACG 1801 AGGAGAAGAA CATCATCACC AATCTGTCCA AGTGCGATTT TACACAGATG TCCCAGTATT 1861 TTAAGGCCCA GACTGAGGCC CGGAAACAGA TGAGCAAGGA AGAGAAGCTG AAGATTAAGG 1921 AGGAGAACGA GAAGCTGCTG AAAGAGTACG GCTTCTGCAT CATGGACAAC CACAAGGAGC 1981 GGATTGCCAA TTTTAAGATC GAGCCCCCTG GCCTGTTTCG GGGCCGGGGG AACCACCCCA 2041 AAATGGGGAT GCTGAAGAGA AGAATCATGC CCGAGGACAT CATTATCAAC TGCAGCAAGG 2101 ACGCCAAGGT GCCTAGCCCT CCACCAGGGC ACAAATGGAA GGAGGTGCGG CACGACAACA 2161 AGGTGACCTG GCTGGTGAGC TGGACCGAGA ACATCCAGGG AAGCATCAAA TATATTATGC 2221 TGAACCCCTC ATCTCGCATC AAAGGCGAGA AGGACTGGCA GAAGTACGAA ACAGCACGCA 2281 GGCTGAAGAA GTGCGTGGAT AAAATCCGCA ACCAGTACAG AGAGGATTGG AAGAGCAAGG 2341 AGATGAAGGT GCGCCAGCGG GCAGTCGCCC TGTATTTCAT CGACAAACTG GCCCTGAGGG 2401 CCGGCAACGA GAAAGAGGAG GGTGAGACCG CTGATACCGT GGGATGCTGC AGCCTGCGCG 2461 TGGAGCACAT CAACCTGCAC CCCGAGCTGG ATGGCCAGGA GTACGTGGTG GAATTCGATT 2521 TTCTGGGCAA GGATTCTATC CGCTATTATA ACAAGGTGCC TGTGGAGAAG AGAGTGTTTA 2581 AGAATCTGCA GCTGTTTATG GAGAATAAGC AGCCAGAGGA TGACCTGTTC GACAGACTGA 2641 ATACAGGAAT CCTGAACAAG CACCTGCAGG ACCTGATGGA AGGACTGACC GCTAAAGTGT 2701 TCCGGACATA TAACGCCTCA ATTACATTGC AGCAGCAGCT CAAGGAACTG ACAGCCCCAG 2761 ACGAGAATAT ACCCGCCAAA ATTCTCTCAT ATAACCGGGC CAATAGAGCT GTTGCGATAC 2821 TCTGTAATCA TCAACGGGCA CCTCCAAAAA CCTTCGAAAA GAGCATGATG AACCTCCAGA 2881 CCAAAATTGA TGCTAAAAAG GAACAACTGG CCGATGCCAG AAGGGATCTC AAAAGTGCTA 2941 AAGCCGACGC TAAGGTGATG AAGGACGCCA AGACCAAAAA GGTCGTTGAG TCCAAAAAGA 3001 AAGCGGTCCA GAGACTGGAA GAACAGCTGA TGAAGTTGGA GGTTCAGGCC ACTGACAGAG 3061 AAGAAAATAA GCAGATTGCA CTCGGGACCT CAAAACTGAA CTATTTGGAC CCCAGGATTA 3121 CCGTTGCCTG GTGTAAAAAG TGGGGAGTGC CCATTGAGAA GATTTACAAC AAAACCCAGA 3181 GGGAAAAATT TGCCTGGGCA ATCGATATGG CGGATGAGGA CTATGAATTT tagctcgagc 3241 ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 3301 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 3361 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 3421 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc 3481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 3541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 3601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 3661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 3721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 3781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 3841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 3901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 3961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 4021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 4081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 4141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 4201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 4261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 4321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 4381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 4441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 4501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 4561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 4801 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 4861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 4921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 4981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 5041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 5101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 5161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 5221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 5281 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 5341 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 5401 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 5461 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg 5521 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc 5581 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 5641 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 5701 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 5761 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 5821 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 5881 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 5941 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 6001 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 6061 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 6121 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 6181 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 6241 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 6301 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 6361 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 6421 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 6481 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 6541 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 6601 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 6661 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 6721 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 6781 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca 6841 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 6901 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 6961 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag 7021 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat 7081 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc 7141 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag 7201 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.