pCMV-SP100-3×HA-Neo plasmid

pCMV-SP100-3×HA-Neo plasmid

PVT90637

pCMV-SP100-3×HA-Neo plasmid Information

Plasmid name: pCMV-SP100(human)-3×HA-Neo
Alias: NM_001080391.2;lysp100b
Gene lenght: 2655bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SP100-3×HA-Neo plasmid Description

pCMV-SP100-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SP100-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gcaggtgggg gcggcgacct gagcaccagg aggctgaatg aatgtatttc 961 accagtagca aatgagatga accatcttcc tgcacacagc cacgatttgc aaaggatgtt 1021 cacggaagac cagggtgtag atgacaggct gctctatgac attgtattca agcacttcaa 1081 aagaaataag gtggagattt caaatgcaat aaaaaagaca tttccattcc tcgagggcct 1141 ccgtgatcgt gatctcatca caaataaaat gtttgaagat tctcaagatt cttgtagaaa 1201 cctggtccct gtacagagag tggtgtacaa tgttcttagt gaactggaga agacatttaa 1261 cctgccagtt ctggaagcac tgttcagcga tgtcaacatg caggaatacc ccgatttaat 1321 tcacatttat aaaggctttg aaaatgtaat ccatgacaaa ttgcctctcc aagaaagtga 1381 agaagaagag agggaggaga ggtctggcct ccaactaagt cttgaacaag gaactggtga 1441 aaactctttt cgaagcctga cttggccacc ttcgggttcc ccatctcatg ctggtacaac 1501 cccacctgaa aatggactct cagagcaccc ctgtgaaaca gaacagataa atgcaaagag 1561 aaaagataca accagtgaca aagatgattc gctaggaagc caacaaacaa atgaacaatg 1621 tgctcaaaag gctgagccaa cagagtcctg cgaacaaatt gctgtccaag tgaataatgg 1681 ggatgctgga agggagatgc cctgcccgtt gccctgtgat gaagaaagcc cagaggcaga 1741 gctacacaac catggaatcc aaattaattc ctgttctgtg cgactggtgg atataaaaaa 1801 ggaaaagcca ttttctaatt caaaagttga gtgccaagcc caagcaagaa ctcatcataa 1861 ccaggcatct gacataatag tcatcagcag tgaggactct gaaggatcca ctgacgttga 1921 tgagccctta gaagtcttca tctcagcacc gagaagtgag cctgtgatca ataatgacaa 1981 ccctttagaa tcaaatgatg aaaaggaggg ccaagaagcc acttgctcac gaccccagat 2041 tgtaccagag cccatggatt tcagaaaatt atctacattc agagaaagtt ttaagaaaag 2101 agtgatagga caagaccacg acttttcaga atccagtgag gaggaggcgc ccgcagaagc 2161 ctcgagcggg gcactgagaa gcaagcatgg tgagaaggct cctatgactt ctagaagtac 2221 atctacttgg agaataccca gcaggaagag acgtttcagc agtagtgact tttcagacct 2281 gagtaatgga gaagagcttc aggaaacctg cagctcatcc ctaagaagag ggtcaggatc 2341 acagccacaa gaacctgaaa ataagaagtg ctcctgtgtc atgtgttttc caaaaggtgt 2401 gccaagaagc caagaagcaa ggactgaaag tagtcaagca tctgacatga tggataccat 2461 ggatgttgaa aacaattcta ctttggaaaa acacagtggg aaaagaagaa aaaagagaag 2521 gcatagatct aaagtaaatg gtctccaaag agggagaaag aaagacagac ctagaaaaca 2581 tttaactctg aataacaaag tccaaaagaa aagatggcaa caaagaggaa gaaaagccaa 2641 cactagacct ttgaaaagaa gaagaaaaag aggtccaaga attcccaaag atgaaaatat 2701 taattttaaa caatctgaac ttcctgtgac ctgtggtgag gtgaagggca ctctatataa 2761 ggagcgattc aaacaaggaa cctcaaagaa gtgtatacag agtgaggata aaaagtggtt 2821 cactcccagg gaatttgaaa ttgaaggaga ccgcggagca tccaagaact ggaagctaag 2881 tatacgctgc ggtggatata ccctgaaagt cctgatggag aacaaatttc tgccagaacc 2941 accaagcaca agaaaaaaga gaatactgga atctcacaac aataccttag ttgacccttg 3001 tccggaaaac tcaaatatat gtgaggtgtg caacaaatgg ggacggctgt tctgctgcga 3061 cacttgtcca agatcctttc atgagcactg ccacatccca tccgtggaag ctaacaagaa 3121 cccgtggagt tgcatcttct gcaggataaa gactattcag gaaagatgcc cagaaagcca 3181 atcaggtcat caggaatctg aagtcctgat gaggcagatg ctgcctgagg agcagttgaa 3241 atgtgaattc ctcctcttga aggtctactg tgattcgaaa agctgctttt tcgcctcaga 3301 accgtattat aacagagagg ggtctcaggg cccacagaag cccatgtggt taaacaaagt 3361 caagacaagt ttgaatgagc agatgtacac ccgagtagaa gggtttgtgc aggacatgcg 3421 tctcatcttt cataaccaca aggaatttta cagggaagat aaattcacca gactgggaat 3481 tcaagtacag gacatctttg agaagaattt cagaaacatt tttgcaattc aggaaacaag 3541 caagaacatt ataatgttta ttCTCGAGTA CCCATACGAC GTACCTGATT ATGCAACTTA 3601 TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT GTCCCAGACT ACGCAtgaGG 3661 GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 3721 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 3781 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 3841 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 3901 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA 3961 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC 4021 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC 4081 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG 4141 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC 4201 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG 4261 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 4321 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 4381 CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 4441 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 4501 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 4561 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 4621 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG 4681 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 4741 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 4801 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 4861 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 4921 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 4981 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 5041 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 5101 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 5161 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 5221 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 5281 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 5341 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 5401 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 5461 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 5521 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 5581 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 5641 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 5701 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 5761 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 5821 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 5881 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 5941 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 6001 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 6061 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 6121 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 6181 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 6241 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 6301 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 6361 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 6421 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 6481 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 6541 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 6601 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 6661 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 6721 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 6781 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 6841 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 6901 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 6961 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 7021 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 7081 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 7141 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 7201 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 7261 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 7321 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 7381 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 7441 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 7501 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 7561 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 7621 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 7681 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 7741 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 7801 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 7861 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 7921 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 7981 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 8041 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG 8101 GAGATCTCCC GATCCCCTAT GGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.