pDC316-mCMV-18HNL01(HPV18)-EGF

pDC316-mCMV-18HNL01(HPV18)-EGFP plasmid

PVT90591

pDC316-mCMV-18HNL01(HPV18)-EGFP plasmid Information

Plasmid name: pDC316-mCMV-18HNL01(HPV18)-EGFP
Alias:
Gene lenght: 1704bp
Host: Mammalian cells,Adenovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human papilloma virus
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: MCMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PDC316.F
Reverse primer:
Copy number: High
Induction:

pDC316-mCMV-18HNL01(HPV18)-EGFP plasmid Description

pDC316-mCMV-18HNL01(HPV18)-EGFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for adenovirus packaging.

pDC316-mCMV-18HNL01(HPV18)-EGFP plasmid Sequence

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac 721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca 781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg 841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc 901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc 961 agtcttcggt ctgaccaccg tagaacgcag atcgaattcG CCACCatgtg cctgtataca 1021 cgggtcctga tattacatta ccatctacta cctctgtatg gcccattgta tcacccacag 1081 cccctgcctc tacacagtat attggtatac atggtacaca ttattatttg tggccattat 1141 attattttat tcctaagaaa cgtaaacgtg ttccctattt ttttgcagat ggctttgtgg 1201 cggcctagtg acaataccgt atatcttcca cctccttctg tggcaagagt tgtaaatacc 1261 gatgattatg tgactcgcac aagcatattt tatcatgctg gcagctctag attattaact 1321 gttggtaatc catattttag ggttcctgca ggtggtggca ataagcagga tattcctaag 1381 gtttctgcat accaatatag agtatttagg gtgcagttac ctgacccaaa taaatttggt 1441 ttacctgata ctagtattta taatcctgaa acacaacgtt tagtgtgggc ctgtgttgga 1501 gtggaaattg gccgtggtca gcctttaggt gttggcctta gtgggcatcc attttataat 1561 aaattagatg acactgaaag ttcccatgcc gccacgtcta atgtttctga ggacgttagg 1621 gacaatgtgt ctgtagatta taagcagaca cagttatgta ttttgggctg tgcccctgct 1681 attggggaac actgggctaa aggcactgct tgtaaatcgc gtcctttatc acagggcgat 1741 tgcccccctt tagaacttaa aaacacagtt ttggaagatg gtgatatggt agatactgga 1801 tatggtgcca tggactttag tacattgcaa gatactaaat gtgaggtacc attggatatt 1861 tgtcagtcta tttgtaaata tcctgattat ttacaaatgt ctgcagatcc ttatggggat 1921 tccatgtttt tttgcttacg gcgtgagcag ctttttgcta ggcatttttg gaatagagca 1981 ggtactatgg gtgacactgt gcctcaatcc ttatatatta aaggcacagg tatgcgtgct 2041 tcacctggca gctgtgtgta ttctccctct ccaagtggct ctattgttac ctctgactcc 2101 cagttgttta ataaaccata ttggttacat aaggcacagg gtcataacaa tggtgtttgc 2161 tggcataatc aattatttgt tactgtggta gataccactc gcagtaccaa tttaacaata 2221 tgtgcttcta cacagtctcc tgtacctggg caatatgatg ctaccaaatt taagcagtat 2281 agcagacatg ttgaggaata tgatttgcag tttatttttc agttgtgtac tattacttta 2341 actgcagatg ttatgtccta tattcatagt atgaatagca gtattttaga ggattggaac 2401 tttggtgttc cccccccgcc aactactagt ttggtggata catatcgttt tgtacaatct 2461 gttgctatta cctgtcaaaa ggatgctgca ccggctgaaa ataaggatcc ctatgataag 2521 ttaaagtttt ggaatgtgga tttaaaggaa aagttttctt tagacttaga tcaatatccc 2581 cttggacgta aatttttggt tcaggctgga ttgcgtcgca agcccaccat aggccctcgc 2641 aaacgttctg ctccatctgc cactacgtct tctaaacctg ccaagcgtgt gcgtgtacgt 2701 gccaggaagG GCGGTGGAGG AAGCggcgga ggtggatcta tggtgagcaa gggcgaggag 2761 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 2821 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 2881 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 2941 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 3001 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 3061 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 3121 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 3181 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 3241 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 3301 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 3361 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 3421 gccgggatca ctctcggcat ggacgagctg tacaagtaga agcttgagct cgtcgacttc 3481 gagcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 3541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 3601 atcttatcat gtctggatcg tctagcatcg aagatccaat aacttcgtat agcatacatt 3661 atacgaagtt ataagtagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 3721 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 3781 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 3841 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3901 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 3961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 4021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 4081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 4141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 4201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 4261 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 4321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 4381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4501 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 4561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4861 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4921 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4981 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 5041 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 5101 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 5161 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 5221 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 5281 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5341 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5401 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5461 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5521 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5581 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5641 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 5701 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 5761 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 5821 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 5881 ctataaaaat aggcgtatca ctctaggcaa aatagcaccc tcccgctcca gaacaacata 5941 cagcgcttca cagcggcagc ctaacagtca gccttaccag taaaaaagaa aacctattaa 6001 aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg 6061 cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc 6121 cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc 6181 aaatcgtcac ttccgttttc ccacgttacg taacttccca ttttaagaaa actacaattc 6241 ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc 6301 gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta 6361 ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.