pCMV-SHCBP1L-EGFP-Neo plasmid

pCMV-SHCBP1L-EGFP-Neo plasmid

PVT90558

pCMV-SHCBP1L-EGFP-Neo plasmid Information

Plasmid name: pCMV-SHCBP1L(human)-EGFP-Neo
Alias: NM_030933.4;C1orf14; GE36
Gene lenght: 1959bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SHCBP1L-EGFP-Neo plasmid Description

pCMV-SHCBP1L-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SHCBP1L-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatgg cgtcgggctc caaggcctcg gtgcccgcgg 961 actcattccg caccatcagc ccggacaggc gaggcgagaa gtccgcctcc gctgtctccg 1021 gggacacggc ggccgcgacc accctgaagg gcaccgcgat cccagtgcgg tcggtggtgg 1081 cctcccctcg cccggtgaag gggaaagcgg gccgggagac ggccaggctg cggctccagc 1141 gcctgcccgc ggctcaggcc gaggacacgg gagaggcggc ggcggcggcg gcggaggagc 1201 ccctgctgcc agtgcctgag gatgaagagg aggcgcagcc cctgccccca gtctgcgtgt 1261 cccgtatgag ggggatgtgg cgggacgaga aggtgtcgct gtattgcgac gaagtgctgc 1321 aggactgtaa ggcagaagat gctgatgaag ttatgggtaa atacctatca gaaaaattaa 1381 agttgaaaga caaatggctt ggagtctgga agactaatcc cagtgtattc tttgtgaaat 1441 atgaagaagc ttctatccct tttgttggta tattggttga ggtaacttgt gaaccatacc 1501 aagactcatc atctcgtttc aaagttactg tttctgttgc tgagcccttt tcttccaaca 1561 ttgcaaatat tccaagagat ttggttgatg agattttgga ggaactggaa cacagtgtgc 1621 ctcttttgga agtgtatcct gttgagggac aagatactga tatacatgtt attgctttgg 1681 ccttggaagt tgtcaggttt ttttatgact ttctttggag agactgggat gatgaagaaa 1741 gttgtgagaa ttatactgca cttattgaag aaagaattaa tttgtggtgt gatatacagg 1801 atggaacaat acctggtcct atcgcacaac gttttaaaaa aactttggag aaatataaaa 1861 acaaacgtgt cgagctcatt gagtatcaga gcaatattaa agaagatcca tctgcagcag 1921 aggctgttga atgctggaaa aaatactatg agatagtcat gctctgtgga ttactgaaaa 1981 tgtgggaaga tttacgcctc cgagttcatg gacctttctt tccaagaatt ctgaggcgta 2041 ggaaaggaaa aagagaattt ggaaagacta taacacatat tgtagcaaaa atgatgacta 2101 ctgaaatgat aaaggattta tcttcagaca cacttctcca acagcatggt gatttggatt 2161 tggctttgga taattgttat agtggagata cagtaataat ttttccagga gaatatcaag 2221 ctgcaaatct tgctttgttg acagatgaca tcattataaa gggagttgga aagagagagg 2281 aaattatgat tacttctgaa ccttctcgtg acagttttgt ggtgtccaaa gctgacaatg 2341 tgaaactaat gcatctatct ttgatacaac aagggacggt tgatggtatc gtggtggtgg 2401 agtctggtca catgactcta gaaaactgca tattaaaatg tgaaggaaca ggagtgtgtg 2461 ttcttacagg ggctgctttg acaattacag acagtgaaat aactggcgcc cagggtgctg 2521 gtgttgaact gtatcctgga agcatagcta ttttggaaag aaatgaaatt catcactgta 2581 ataacctcag aaccagtaac agttcaaaaa gcaccttagg tggagttaat atgaaggttc 2641 ttccagcacc caaattgaag atgactaata atcatattta tagcaacaaa ggctatggag 2701 taagcattct tcaaccaatg gaacagtttt ttatcgtagc agaagaagct ctcaacaaaa 2761 gggcttcttc aggagataaa aaagatgata aaatgctctt caaagtaatg caaaatctga 2821 atctggaaat gaataataat aagatagaag caaacgtcaa gggggatatc agaatagtca 2881 caagtTCctc gagtggcggt ggaggaagcg gcggaggtgg atctatggtg agcaagggcg 2941 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 3001 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 3061 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 3121 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 3181 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 3241 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 3301 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 3361 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 3421 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 3481 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 3541 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 3601 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtagtctaga gggcccgttt 3661 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 3721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 3781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 3841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 3901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 3961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 4021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 4081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 4141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 4201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4261 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4321 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4381 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 4441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 4501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 4561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 4621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 4681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 4741 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 4801 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 4861 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 4921 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 4981 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 5041 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 5101 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 5161 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 5221 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 5281 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 5341 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 5401 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 5461 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 5521 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 5581 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 5641 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 5701 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 5761 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 5821 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 5881 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 5941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 6001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 6061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 6121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 6181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 6361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 6421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 6541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 6661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 6721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 6781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 6841 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 6901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 6961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 7021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 7081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 7141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 7201 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 7261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 7321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 7381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 7441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 7501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 7561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 7621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 7681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 7741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 7801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 7861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 7921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 7981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 8041 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.