pCMV-LRP6-GFP plasmid

pCMV-LRP6-GFP plasmid

PVT90516

pCMV-LRP6-GFP plasmid Information

Plasmid name: pCMV-LRP6(human)-GFP
Alias: NM_002336.3;ADCAD2; STHAG7
Gene lenght: 4839bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-LRP6-GFP plasmid Description

pCMV-LRP6-GFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-LRP6-GFP plasmid Sequence

1 ctagtaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt 421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg 541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg 601 gactcagatc tcgagctcaa gcttcgaatt cgccaccatg ggggccgtcc tgaggagcct 661 cctggcctgc agcttctgtg tgctcctgag agcggcccct ttgttgcttt atgcaaacag 721 acgggacttg cgattggttg atgctacaaa tggcaaagag aatgctacga ttgtagttgg 781 aggcttggag gatgcagctg cggtggactt tgtgtttagt catggcttga tatactggag 841 tgatgtcagc gaagaagcca ttaaacgaac agaatttaac aaaactgaga gtgtgcagaa 901 tgttgttgtt tctggattat tgtcccccga tgggctggca tgtgattggc ttggagaaaa 961 attgtactgg acagattctg aaactaatcg gattgaagtt tctaatttag atggatcttt 1021 acgaaaagtt ttattttggc aagagttgga tcaacccaga gctattgcct tagatccttc 1081 aagtgggttc atgtactgga cagactgggg agaagtgcca aagatagaac gtgctggaat 1141 ggatggttca agtcgcttca ttataataaa cagtgaaatt tactggccaa atggactgac 1201 tttggattat gaagaacaaa agctttattg ggcagatgca aaacttaatt tcatccacaa 1261 atcaaatctg gatggaacaa atcggcaggc agtggttaaa ggttcccttc cacatccttt 1321 tgccttgacg ttatttgagg acatattgta ctggactgac tggagcacac actccatttt 1381 ggcttgcaac aagtatactg gtgagggtct gcgtgaaatc cattctgaca tcttctctcc 1441 catggatata catgccttca gccaacagag gcagccaaat gccacaaatc catgtggaat 1501 tgacaatggg ggttgttccc atttgtgttt gatgtctcca gtcaagcctt tttatcagtg 1561 tgcttgcccc actggggtca aactcctgga gaatggaaaa acctgcaaag atggtgccac 1621 agaattattg cttttagctc gaaggacaga cttgagacgc atttctttgg atacaccaga 1681 ttttacagac attgttctgc agttagaaga catccgtcat gccattgcca tagattacga 1741 tcctgtggaa ggctacatct actggactga tgatgaagtg agggccatac gccgttcatt 1801 tatagatgga tctggcagtc agtttgtggt cactgctcaa attgcccatc ctgatggtat 1861 tgctgtggac tgggttgcac gaaatcttta ttggacagac actggcactg atcgaataga 1921 agtgacaagg ctcaatggga ccatgaggaa gatcttgatt tcagaggact tagaggaacc 1981 ccgggctatt gtgttagatc ccatggttgg gtacatgtat tggactgact ggggagaaat 2041 tccgaaaatt gagcgagcag ctctggatgg ttctgaccgt gtagtattgg ttaacacttc 2101 tcttggttgg ccaaatggtt tagccttgga ttatgatgaa ggcaaaatat actggggaga 2161 tgccaaaaca gacaagattg aggttatgaa tactgatggc actgggagac gagtactagt 2221 ggaagacaaa attcctcaca tatttggatt tactttgttg ggtgactatg tttactggac 2281 tgactggcag aggcgtagca ttgaaagagt tcataaacga agtgcagaga gggaagtgat 2341 catagatcag ctgcctgacc tcatgggcct aaaggctaca aatgttcatc gagtgattgg 2401 ttccaacccc tgtgctgagg aaaacggggg atgtagccat ctctgcctct atagacctca 2461 gggccttcgc tgtgcttgcc ctattggctt tgaactcatc agtgacatga agacctgcat 2521 tgtcccagag gctttccttt tgttttcacg gagagcagat atcagacgaa tttctctgga 2581 aacaaacaat aataatgtgg ctattccact cactggtgtc aaagaagctt ctgctttgga 2641 ttttgatgtg acagacaacc gaatttattg gactgatata tcactcaaga ccatcagcag 2701 agcctttatg aatggcagtg cactggaaca tgtggtagaa ttcggcttag attatccaga 2761 aggcatggca gtagactggc ttgggaagaa cttgtactgg gcagacacag gaacgaatcg 2821 aattgaggtg tcaaagttgg atgggcagca ccgacaagtt ttggtgtgga aagacctaga 2881 tagtcccaga gctctcgcgt tggaccctgc cgaaggattt atgtattgga ctgaatgggg 2941 tggaaaacct aagatagaca gagctgcaat ggatggaagt gaacgtacta ccttagttcc 3001 aaatgtgggg cgggcaaacg gcctaactat tgattatgct aaaaggaggc tttattggac 3061 agacctggac accaacttaa tagaatcttc aaatatgctt gggctcaacc gtgaagttat 3121 agcagatgac ttgcctcatc cttttggctt aactcagtac caagattata tctactggac 3181 ggactggagc cgacgcagca ttgagcgtgc caacaaaacc agtggccaaa accgcaccat 3241 cattcagggc catttggatt atgtgatgga catcctcgtc tttcactcat ctcgacagtc 3301 agggtggaat gaatgtgctt ccagcaatgg gcactgctcc cacctctgct tggctgtgcc 3361 agttgggggt tttgtttgtg gatgccctgc ccactactct cttaatgctg acaacaggac 3421 ttgtagtgct cctacgactt tcctgctctt cagtcaaaag agtgccatca accgcatggt 3481 gattgatgaa caacagagcc ccgacatcat ccttcccatc cacagccttc ggaatgtccg 3541 ggccattgac tatgacccac tggacaagca actctattgg attgactcac gacaaaacat 3601 gatccgaaag gcacaagaag atggcagcca gggctttact gtggttgtga gctcagttcc 3661 gagtcagaac ctggaaatac aaccctatga cctcagcatt gatatttaca gccgctacat 3721 ctactggact tgtgaggcta ccaatgtcat taatgtgaca agattagatg ggagatcagt 3781 tggagtggtg ctgaaaggcg agcaggacag acctcgagcc gttgtggtaa acccagagaa 3841 agggtatatg tattttacca atcttcagga aaggtctcct aaaattgaac gggctgcttt 3901 ggatgggaca gaacgggagg tcctcttttt cagtggctta agtaaaccaa ttgctttagc 3961 ccttgatagc aggctgggca agctcttttg ggctgattca gatctccggc gaattgaaag 4021 cagtgatctc tcaggtgcta accggatagt attagaagac tccaatatct tgcagcctgt 4081 gggacttact gtgtttgaaa actggctcta ttggattgat aaacagcagc aaatgattga 4141 aaaaattgac atgacaggtc gagagggtag aaccaaagtc caagctcgaa ttgcccagct 4201 tagtgacatt catgcagtaa aggagctgaa ccttcaagaa tacagacagc acccttgtgc 4261 tcaggataat ggtggctgtt cacatatttg tcttgtaaag ggggatggta ctacaaggtg 4321 ttcttgcccc atgcacctgg ttctacttca agatgagcta tcatgtggag aacctccaac 4381 atgttctcct cagcagttta cttgtttcac gggggaaatt gactgtatcc ctgtggcttg 4441 gcggtgcgat gggtttactg aatgtgaaga ccacagtgat gaactcaatt gtcctgtatg 4501 ctcagagtcc cagttccagt gtgccagtgg gcagtgtatt gatggtgccc tccgatgcaa 4561 tggagatgca aactgccagg acaaatcaga tgagaagaac tgtgaagtgc tttgtttaat 4621 tgatcagttc cgctgtgcca atggtcagtg cattggaaag cacaagaagt gtgatcataa 4681 tgtggattgc agtgacaagt cagatgaact ggattgttat ccgactgaag aaccagcacc 4741 acaggccacc aatacagttg gttctgttat tggcgtaatt gtcaccattt ttgtgtctgg 4801 aactgtatac tttatctgcc agaggatgtt gtgtccacgt atgaagggag atggggaaac 4861 tatgactaat gactatgtag ttcatggacc agcttctgtg cctcttggtt atgtgccaca 4921 cccaagttct ttgtcaggat ctcttccagg aatgtctcga ggtaaatcaa tgatcagctc 4981 cctcagtatc atggggggaa gcagtggacc cccctatgac cgagcccatg ttacaggagc 5041 atcatcaagt agttcttcaa gcaccaaagg cacttacttc cctgcaattt tgaaccctcc 5101 accatcccca gccacagagc gatcacatta cactatggaa tttggatatt cttcaaacag 5161 tccttccact cataggtcat acagctacag gccatatagc taccggcact ttgcaccccc 5221 caccacaccc tgcagcacag atgtttgtga cagtgactat gctcctagtc ggagaatgac 5281 ctcagtggca acagccaagg gctataccag tgacttgaac tatgattcag aacctgtgcc 5341 cccacctccc acaccccgaa gccaatactt gtcagcagag gagaactatg aaagctgccc 5401 accttctcca tacacagaga ggagctattc tcatcacctc tacccaccgc caccctctcc 5461 ctgtacagac tcctccgGaG GCGGAGGTAG TGGAGGAGGG GGAAGCtcac cggtcgccac 5521 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 5581 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 5641 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 5701 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 5761 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 5821 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 5881 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 5941 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 6001 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 6061 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 6121 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 6181 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 6241 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa 6301 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat 6361 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 6421 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 6481 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc 6541 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca 6601 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt 6661 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt 6721 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga 6781 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6841 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6901 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6961 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac 7021 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 7081 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 7141 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct 7201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 7261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc 7321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 8101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 8161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 8221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 8281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 8341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 8401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 9121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 9181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 9241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9301 cacctgggtc gacattgatt attga

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.