pLV3-CMV-CHIT1-3×FLAG-CopGFP-P

pLV3-CMV-CHIT1-3×FLAG-CopGFP-Puro plasmid

PVT90499

pLV3-CMV-CHIT1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CHIT1(human)-3×FLAG-CopGFP-Puro
Alias: NM_003465.3;CHI3; CHIT; CHITD
Gene lenght: 1398bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CHIT1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CHIT1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CHIT1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg tgcggtctgt ggcctgggca 2341 ggtttcatgg tcctgctgat gatcccatgg ggctctgctg caaaactggt ctgctacttc 2401 accaactggg cccagtacag acagggggag gctcgcttcc tgcccaagga cttggacccc 2461 agcctttgca cccacctcat ctacgccttc gctggcatga ccaaccacca gctgagcacc 2521 actgagtgga atgacgagac tctctaccag gagttcaatg gcctgaagaa gatgaatccc 2581 aagctgaaga ccctgttagc catcggaggc tggaatttcg gcactcagaa gttcacagat 2641 atggtagcca cggccaacaa ccgtcagacc tttgtcaact cggccatcag gtttctgcgc 2701 aaatacagct ttgacggcct tgaccttgac tgggagtacc caggaagcca ggggagccct 2761 gccgtagaca aggagcgctt cacaaccctg gtacaggact tggccaatgc cttccagcag 2821 gaagcccaga cctcagggaa ggaacgcctt cttctgagtg cagcggttcc agctgggcag 2881 acctatgtgg atgctggata cgaggtggac aaaatcgccc agaacctgga ttttgtcaac 2941 cttatggcct acgacttcca tggctcttgg gagaaggtca cgggacataa cagccccctc 3001 tacaagaggc aagaagagag tggtgcagca gccagcctca acgtggatgc tgctgtgcaa 3061 cagtggctgc agaaggggac ccctgccagc aagctgatcc ttggcatgcc tacctacgga 3121 cgctccttca cactggcctc ctcatcagac accagagtgg gggccccagc cacagggtct 3181 ggcactccag gccccttcac caaggaagga gggatgctgg cctactatga agtctgctcc 3241 tggaaggggg ccaccaaaca gagaatccag gatcagaagg tgccctacat cttccgggac 3301 aaccagtggg tgggctttga tgatgtggag agcttcaaaa ccaaggtcag ctatctgaag 3361 cagaagggac tgggcggggc catggtctgg gcactggact tagatgactt tgccggcttc 3421 tcctgcaacc agggccgata ccccctcatc cagacgctac ggcaggaact gagtcttcca 3481 tacttgcctt caggcacccc agagcttgaa gttccaaaac caggtcagcc ctctgaacct 3541 gagcatggcc ccagccctgg acaagacacg ttctgccagg gcaaagctga tgggctctat 3601 cccaatcctc gggaacggtc cagcttctac agctgtgcag cggggcggct gttccagcaa 3661 agctgcccga caggcctggt gttcagcaac tcctgcaaat gctgcacctg gaatggatcc 3721 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3781 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3841 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 3901 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 3961 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 4021 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4081 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4141 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4201 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4261 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4321 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4381 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4441 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4501 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4561 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4621 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4681 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4741 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4801 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4861 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4921 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4981 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 5041 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 5101 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5161 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5221 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5281 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5341 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5401 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5461 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5521 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5581 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5641 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5701 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5761 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 5821 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 5881 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 5941 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6001 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6061 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6121 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6181 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6241 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6301 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6361 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6421 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6481 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6541 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6601 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6661 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6721 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6841 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 6901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 6961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 7021 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 7081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8221 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8281 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8341 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8401 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8461 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8521 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8581 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8641 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8701 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8761 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8821 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8881 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8941 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9001 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9061 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9181 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9241 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9301 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9361 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9421 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9481 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9541 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9601 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9661 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.