pHAGE-CMV-EPHA4-FLAG-Puro plas

pHAGE-CMV-EPHA4-FLAG-Puro plasmid

PVT90483

pHAGE-CMV-EPHA4-FLAG-Puro plasmid Information

Plasmid name: pHAGE-CMV-EPHA4(human)-FLAG-Puro
Alias: NM_001304536.2;EK8; HEK8; SEK; TYRO1
Gene lenght: 2958bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pHAGE-CMV-EPHA4-FLAG-Puro plasmid Description

pHAGE-CMV-EPHA4-FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pHAGE-CMV-EPHA4-FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaccgtca gatcgcctgg 2761 agacgccatc cacgctgttt tgacctccat agaagacacc ggcggccacg cgtCGAAGCC 2821 CGGGCGGATC GGGTTTAAAC GGATCCGCCA CCatggctgg gattttctat ttcgccctat 2881 tttcgtgtct cttcgggatt tgcgacgctg tcacaggttc cagggtatac cccgcgaatg 2941 aagttacctt attggattcc agatctgttc agggagaact tgggtggata gcaagccctc 3001 tggaaggagg gtgggaggaa gtgagtatca tggatgaaaa aaatacacca atccgaacct 3061 accaagtgtg caatgtgatg gaacccagcc agaataactg gctacgaact gattggatca 3121 cccgagaagg ggctcagagg gtgtatattg agattaaatt caccttgagg gactgcaata 3181 gtcttccggg cgtcatgggg acttgcaagg agacgtttaa cctgtactac tatgaatcag 3241 acaacgacaa agagcgtttc atcagagaga accagtttgt caaaattgac accattgctg 3301 ctgatgagag cttcacccaa gtggacattg gtgacagaat catgaagctg aacaccgaga 3361 tccgggatgt agggccatta agcaaaaagg ggttttacct ggcttttcag gatgtggggg 3421 cctgcatcgc cctggtatca gtccgtgtgt tctataaaaa gtgtccactc acagtccgca 3481 atctggccca gtttcctgac accatcacag gggctgatac gtcttccctg gtggaagttc 3541 gaggctcctg tgtcaacaac tcagaagaga aagatgtgcc aaaaatgtac tgtggggcag 3601 atggtgaatg gctggtaccc attggcaact gcctatgcaa cgctgggcat gaggagcgga 3661 gcggagaatg ccaagcttgc aaaattggat attacaaggc tctctccacg gatgccacct 3721 gtgccaagtg cccaccccac agctactctg tctgggaagg agccacctcg tgcacctgtg 3781 accgaggctt tttcagagct gacaacgatg ctgcctctat gccctgcacc cgtccaccat 3841 ctgctcccct gaacttgatt tcaaatgtca acgagacatc tgtgaacttg gaatggagta 3901 gccctcagaa tacaggtggc cgccaggaca tttcctataa tgtggtatgc aagaaatgtg 3961 gagctggtga ccccagcaag tgccgaccct gtggaagtgg ggtccactac accccacagc 4021 agaatggctt gaagaccacc aaagtctcca tcactgacct cctagctcat accaattaca 4081 cctttgaaat ctgggctgtg aatggagtgt ccaaatataa ccctaaccca gaccaatcag 4141 tttctgtcac tgtgaccacc aaccaagcag caccatcatc cattgctttg gtccaggcta 4201 aagaagtcac aagatacagt gtggcactgg cttggctgga accagatcgg cccaatgggg 4261 taatcctgga atatgaagtc aagtattatg agaaggatca gaatgagcga agctatcgta 4321 tagttcggac agctgccagg aacacagata tcaaaggcct gaaccctctc acttcctatg 4381 ttttccacgt gcgagccagg acagcagctg gctatggaga cttcagtgag cccttggagg 4441 ttacaaccaa cacagtgcct tcccggatca ttggagatgg ggctaactcc acagtccttc 4501 tggtctctgt ctcgggcagt gtggtgctgg tggtaattct cattgcagct tttgtcatca 4561 gccggagacg gagtaaatac agtaaagcca aacaagaagc ggatgaagag aaacatttga 4621 atcaaggtgt aagaacatat gtggacccct ttacgtacga agatcccaac caagcagtgc 4681 gagagtttgc caaagaaatt gacgcatcct gcattaagat tgaaaaagtt ataggagttg 4741 gtgaatttgg tgaggtatgc agtgggcgtc tcaaagtgcc tggcaagaga gagatctgtg 4801 tggctatcaa gactctgaaa gctggttata cagacaaaca gaggagagac ttcctgagtg 4861 aggccagcat catgggacag tttgaccatc cgaacatcat tcacttggaa ggcgtggtca 4921 ctaaatgtaa accagtaatg atcataacag agtacatgga gaatggctcc ttggatgcat 4981 tcctcaggaa aaatgatggc agatttacag tcattcagct ggtgggcatg cttcgtggca 5041 ttgggtctgg gatgaagtat ttatctgata tgagctatgt gcatcgtgat ctggccgcac 5101 ggaacatcct ggtgaacagc aacttggtct gcaaagtgtc tgattttggc atgtcccgag 5161 tgcttgagga tgatccggaa gcagcttaca ccaccagggg tggcaagatt cctatccggt 5221 ggactgcgcc agaagcaatt gcctatcgta aattcacatc agcaagtgat gtatggagct 5281 atggaatcgt tatgtgggaa gtgatgtcgt acggggagag gccctattgg gatatgtcca 5341 atcaagatgt gattaaagcc attgaggaag gctatcggtt accccctcca atggactgcc 5401 ccattgcgct ccaccagctg atgctagact gctggcagaa ggagaggagc gacaggccta 5461 aatttgggca gattgtcaac atgttggaca aactcatccg caaccccaac agcttgaaga 5521 ggacagggac ggagagctcc agacctaaca ctgccttgtt ggatccaagc tcccctgaat 5581 tctctgctgt ggtatcagtg ggcgattggc tccaggccat taaaatggac cggtataagg 5641 ataacttcac agctgctggt tataccacac tagaggctgt ggtgcacgtg aaccaggagg 5701 acctggcaag aattggtatc acagccatca cgcaccagaa taagattttg agcagtgtcc 5761 aggcaatgcg aacccaaatg cagcagatgc acggcagaat ggttcccgtc TCTAGAGACT 5821 ACAAGGACGA CGATGACAAG TAGTCGAGAA TTCTACCggg taggggaggc gcttttccca 5881 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc 5941 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 6001 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag 6061 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg 6121 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt 6181 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg 6241 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 6301 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 6361 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 6421 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 6481 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 6541 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 6601 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 6661 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 6721 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 6781 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 6841 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 6901 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 6961 gcaagcccgg tgcctgaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa 7021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 7081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 7141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 7201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 7261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 7321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 7381 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 7441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7501 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 7561 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag 7621 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 7681 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 7741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7861 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 7921 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg 7981 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 8041 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 8101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 8161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 8221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 8281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 8341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 8401 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa 8461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 8521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 8581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 8641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 8701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 8761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 8821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 8881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 8941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 9001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 9061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 9121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 9181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 9241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 9301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 9361 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 9421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 9481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 9541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 9601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 9661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 9721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 9781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 9841 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 9901 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 9961 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 10021 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta 10081 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 10141 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg 10201 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 10261 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 10321 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg 10381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 10441 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 10501 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 10561 agaaattgta tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.