pLV3-CMV-EGFP-Atg2a-3×FLAG-Bla

pLV3-CMV-EGFP-Atg2a-3×FLAG-Blast plasmid

PVT89876

pLV3-CMV-EGFP-Atg2a-3×FLAG-Blast plasmid Information

Plasmid name: pLV3-CMV-EGFP-Atg2a(rat)-3×FLAG-Blast
Alias: NM_001109545.1;
Gene lenght: 5748bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-EGFP-Atg2a-3×FLAG-Blast plasmid Description

pLV3-CMV-EGFP-Atg2a-3×FLAG-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EGFP-Atg2a-3×FLAG-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG 2341 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG 2401 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG 2461 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC 2521 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC 2581 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG 2641 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG 2701 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC 2761 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG 2821 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC 2881 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC 2941 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC 3001 ATGGACGAGC TGTACAAGgg tggcggtggc tcgggcggtg gtgggtcggg tggcggcgga 3061 tctatcgatg aattggaatt catgtcacga tggctgtggc catggtcgaa ctgtgtgaaa 3121 gagcgtgtct gccgctacct gctacagcac tacttgggtc acttctttca ggagcacctc 3181 agcctggacc agctcagcct ggatctgtac aagggcagcg ttgtcctgcg ggatattcac 3241 ctggaaacct ggtctgtgaa cgaggttttg aggtcaatgg agtcaccact ggaactggtg 3301 gaaggcttcg tgagctccat cgaggtggct gtcccctggg ctgcacttct cactgaccac 3361 tgcactgtgt gtgtgtcagg ccttcagctt atcctgcaac cccgccaggg ttcagggcca 3421 gaggctgcag actctcagac ttgggcctca tgcatgacca caagcctaca gctggctcaa 3481 gagtgccttc gggaagggtt gccagaaccc tcagaaccac cacagcccct agaggggctg 3541 gaaatgtttg cacagaccat tgagactgtg ctccggagga tcaaggtgac cttcctgaac 3601 actgttgtga gggtggagca ttcactgggt gatgaggaac ggagtgtagc tgtggaggtc 3661 cgagtgcaga gactggaata ctgtgacgaa gcagtgcggg acccaagcca ggcaccacct 3721 gtggacgtgc accagccacc tgccttcttg cacaagctgc tgcagctgac tggggtctgc 3781 ctgtactttg aagagctgcc ctcccaggtg gacccgccaa agccaccttt gcaaatcggc 3841 agctgcacag ggtacatgga gctcatggtg aagctgaaac agaatgaggc cttcccgggg 3901 cccaagttgg aggtatcagg acagctggga tccctgcatc tgctcttgac cccgcgtcag 3961 ctccagcagc tccaaaaact gctcagtgcc atcaaccttg cagatccagg aggcctggct 4021 gacaagctga acaagagccg cccactaggt gctgaagact tgtggctcat tgagcaagat 4081 cttaaccagc agctgcaggc gggtgcagtg gctgagtccc tcagcctgca ccccatcacc 4141 agccccctca atttggacag tgcagaccta ttcttctcca tggctggcct cacaagcagt 4201 gtgacctccg gtgtctctga gctctcagtg tatagtgtag acctgggctc ctcggttcac 4261 agcaacatgg ccctccatcg accctcaacc ccaacccact caggtggcaa gacggctccg 4321 actccccttc tggataccat gcgccctgac tcactggtga agatgacctt gggaggtttg 4381 agcctgacct tgcttcagac agcttcccca tcttcaggat cacctgacct caccacccac 4441 ttttttgctg agttcgatgc tgccaaagat gggccgttcg ggtcccgaga cttctaccac 4501 ctccggccac gcttccagag ggcctgccct tgtagccatg tccgattaac aggcacagcc 4561 gtgcagctgt cctgggagct gcgtactggc ggccacagcc ggaggacaag cagcacagaa 4621 gtgcacttcg gacagctgga ggtgctggag tgcctgtggc ccagggctgc ctcagagcca 4681 gagtacacag agatcctgag cttccccagt tactctggct ccgaagcctc agctcggccc 4741 tgcgcccatc tgcgccacac acaaaccttt cgccgggtgc tcaagagccg gtcccggcgc 4801 tccactgcct gccattgtca ctcagaactc tccctggacc tggctgattt ccaagctgac 4861 gtggagctgg ggtcgctgga ccgccttgct gtcttgctcc gtcaaatcac cacaccctct 4921 gagccacccg ctggcctact aacagagccc ccacaggcca ctgagcagca gacagtgttt 4981 cgtctctcag caccccgggc caccctgagg ctacgcttcc ccatcccaga cttacggcct 5041 gaacgggacc cctgggctgg ccaggctgta cggactgagc agctgcgact ggagctgagt 5101 gagcctcagt tccgctcaga gctcaacagt gggcctggcc ccccagctcc tacccgcttg 5161 gaacttactt gcttagacct acaaggaatc tacgaagatg gagagaagcc acctgtcccc 5221 tgcctgcggg tctctaaggc tctggatccc aggagcactg aggccaagta cttcctacct 5281 caggtagtgg tgaccctgaa cccccagtcc agtggcacac agtgggagac agcctctgag 5341 aaaggccggg atctagaact gtctaccgag agtccatgtg agctccagca gcctgagccc 5401 tcacctttct cctccaagcg gactatgtat gaaactgagg agatggtgat ccctggagac 5461 cctgaggaga tgagggcatt ccagaaccgg acgctggcgc tgtcccgctg tactctggat 5521 gtgatcatgc ccagtgccca catcttcctg cccagcaagg aggtctacga aagcatctac 5581 aacaggatca acaatgacct gctcatgtgg gagcctgcag acctacttcc cacatccacc 5641 actgctgctc ggccgcccgg ctcttctggg ttcaagatgt gcaagtcagc cttcaagcta 5701 gactcggatt cggatgaaga agacacccag ttcttctcga tggcatcagg tgccccccag 5761 actcctgccc cggaaccttc ccatcgtcag tctcagagta ccttctccac actggtgaca 5821 gtgctgaagg gtcggatcac tgcgctctgt gaggctaagg atgagacagg gaagcggctg 5881 gatgccactc acggggagct ggtactagat gtggagcaag gcaccatctt cagcgtagcc 5941 cagtaccgtg gccagccggg gctcggctac ttctgcttgg aagctgaaaa ggcaacactc 6001 taccatcgag cggccattga ggactacctg ctgcccactc acttggaagt acccagcttt 6061 gctcccccag ctcagctggc tccaaccatc tacccatcag aggaaggggt gactgagcgg 6121 ggaacattgg gccgcaagag ccagggtccc cccatgctgt ctgcagctgt ccgcatccac 6181 ctggaccccc acaagaatgt caaggaattt ttggtgacaa tacggctgca caaagccact 6241 ctacgccact acatggcccc tcctgagcag agctggcact cccagctatt ggacttcttg 6301 gatgtactgg atgaccctgt gctgggttac ctgcccccaa cagtcatcac tattctgcac 6361 acacacctct tctcctgtgc tgtggattac aggcccctct acctccctgt gcgtgtcctt 6421 atcactgctg agaccttcac tctctccagc aacattgtca tggacacgtc gaccttcctg 6481 ctcaggttca tcctcgatga ctccgctctg tacctgtctg acaagtgtga ggtggagaac 6541 ctggacctgc ggcgagatta tgtctgcgtc ctggacattg acctcctgga gcttgtaatc 6601 aaaacctgga agggcagcac cgagggcaga ctgagccagc cgctgtttga gctgcgctgc 6661 tccaacaatg tggtgcatgt acacagctgt gctgactcct gcgccctgct ggtcaacctg 6721 ctccagtatg taactagctc cggtgacctg catcccccgc cccagccccc cagccctacg 6781 gagatcgccg gccagaagct ttcagagagc ccggcctccc tgccttcctg cctaccagtg 6841 gagacagccc tcatcaacca gcgagacctg actgatgccc ttctggacac tgaacggcgt 6901 agcctacggg agctggccca gtcatcaggt ggcccacttc ctcaggcctc tcccgtctcc 6961 gtctatctgt tcccaggtga acggagtggg gcccaggccc ccttgcctcc tcctgggggc 7021 ccttctcata ccttggggtc ctgttctgag gcaaaggaac atgacaaaga agaggaaggg 7081 gatggagaca ccctggacag tgacgagttc tgcatccttg acgcccctgg cctaggcatt 7141 gcgccccgag acggtgagcc catcgtgact cagctgcatc caggccccat cgttgtgcat 7201 gatgggcact tctcccagcc cctgggaagc actgacctgc tgcgggcgcc tgcccacttc 7261 ccagtgccca gcagccgtgt ggtactacgg gaggtctcct tcatctggca cctttatggg 7321 ggtagagact ttggccttca ccccacttac agagcaagag taggcctcgc aggccctagg 7381 gtctcacctt cccgttcctc tggtcccaac cggccacaga actcctggcg tacccaaggt 7441 ggcattggac ggcaacacca ggtcctcatg gagatccagc tcagcaaagt gagcttccaa 7501 catgaagtat acccagaaga atcagccata gccgggggcc tggaccagga actggatgag 7561 cgaccactgt cccgccaggt gctcattgtt caggagctag aaatccgaga ccggctggcc 7621 acctccaaga tcaacaagtt ccttcatttg cacacgagcg agcggctgcc acggcgcaca 7681 cactccaaca tgcttaccat caaggcactg cacgtggctc ccaccggcag tgtaggaggg 7741 ccggagtgct gtcttcgagt ctccatgatg cctctgaggc tcaatgtgga ccaggatgcc 7801 cttttcttcc tcaaggactt tttcaccagc ctagcagcca gcatcaaccc catggtccca 7861 ggggacactt ttgaggctca ccgagagacc cacagccggc ccagcagccc ccaggaaggg 7921 cagtctgagg gcacagagac agccagcagc ccccaggaag ctccaggcag tagccacagc 7981 tcctctgacc aacagcccat ctacttcagg gagttccgct tcacatctga ggtgcccatc 8041 tgcctggatt accatggcaa acatgtcact gtggaccagg tgggcacttt catgggactc 8101 ctaattggcc tggcccagct caactgctcc gagctgaagc tcaagaggct ttgctgccgg 8161 catgggctcc taggtgtgga caaagtgctc ggctatgccc tcaatgaatg gctgcaggac 8221 atccgaaaga accagctgcc tggcctgctg ggaggcgtgg gtcccatgca ttcggttgta 8281 caactcttcc aggggttccg ggacctgctg tggctaccca tcgagcagta caggaaagat 8341 gggcgcctca ttcgggggct gcagcgtggc gctgcctcct ttgggtcatc cacagcctct 8401 gctgctctgg aactgagcaa caggctggtc caggccatcc aggctacagc tgagacagta 8461 tatgacatcc tgtcccctgc atctcccatc tcccgatccc tacaagacaa acgttcttca 8521 cggaaactac gcagaggtca acaacctgct gacctccggg aaggcatggc taaggcatat 8581 gacgctgttc gagagggtat cctggataca gctcaaacca tctgtgatgt ggcatcaagg 8641 ggccatgagc agaagggact gacaggtgcc gtggggggtg tgatccgaca gctcccccca 8701 actgtggtaa aaccaataat tgtagcaaca gaggcaacat ccaacgtgct agggggcatg 8761 cgtaaccaga tcctgccaga tgcacacaag gaccatgctc tcaagtggcg attggaggag 8821 acccaggacG GATCCGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC 8881 TACAAGGATG ACGATGACAA GtaGCGGCCG CGAAGGATCT GCGATCGCTC CGGTGCCCGT 8941 CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT 9001 TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG 9061 CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC 9121 GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC 9181 TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT 9241 CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG 9301 CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG 9361 CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT 9421 CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGGCCACCA TGGCCAAGCC 9481 TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC 9541 CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC 9601 TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC 9661 TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG 9721 GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT 9781 CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT 9841 GCCCTCTGGT TATGTGTGGG AGGGCTAAGT CGACAATCAA CCTCTGGATT ACAAAATTTG 9901 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC 9961 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA 10021 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT 10081 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA 10141 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC 10201 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT 10261 GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG 10321 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG 10381 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT 10441 CTCCCTTTGG GCCGCCTCCC CGCCTGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG 10501 TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC 10561 GAAAATAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 10621 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 10681 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 10741 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT 10801 ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT 10861 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 10921 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC 10981 TATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 11041 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG 11101 AGGCTTTTTT GGAGGCCTAG ACTTTTGCAG AGACGGCCCA AATTCGTAAT CATGGTCATA 11161 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 11221 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 11281 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 11341 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 11401 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 11461 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 11521 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 11581 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 11641 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 11701 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 11761 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 11821 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 11881 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 11941 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 12001 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 12061 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 12121 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 12181 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 12241 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 12301 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 12361 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 12421 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 12481 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 12541 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 12601 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 12661 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 12721 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 12781 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 12841 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 12901 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 12961 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 13021 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 13081 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 13141 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 13201 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 13261 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC 13321 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC 13381 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 13441 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 13501 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 13561 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC 13621 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG 13681 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC 13741 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.