pLV3-CMV-Tas1r2-EF1a-CopGFP-Pu

pLV3-CMV-Tas1r2-EF1a-CopGFP-Puro plasmid

PVT89442

pLV3-CMV-Tas1r2-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Tas1r2(mouse)-EF1a-CopGFP-Puro
Alias: NM_031873.1;Gpr71; T1r2; TR2
Gene lenght: 2532bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Tas1r2-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Tas1r2-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Tas1r2-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gaccccaggc gaggacactc 2341 catttgctgt ttctcctgct gcatgctctg cctaagccag tcatgctggt agggaactcc 2401 gactttcacc tggctgggga ctacctcctg ggtggcctct ttaccctcca tgccaacgtg 2461 aagagcgtct ctcacctcag ctacctgcag gtgcccaagt gcaatgagta caacatgaag 2521 gtcttgggct acaacctcat gcaggccatg cgattcgccg tggaggaaat caacaactgt 2581 agctctctgc tgcccggcgt gctgctcggc tacgagatgg tggatgtctg ctacctctcc 2641 aacaatatcc agcctgggct ctacttcctg tcacagatag atgacttcct gcccatcctc 2701 aaagactaca gccagtacag gccccaagtg gtggccgtca ttggcccaga caactctgag 2761 tccgccatca ccgtgtccaa cattctctcc tacttcctcg tgccacaggt cacatatagc 2821 gccatcaccg acaagctgcg agacaagcgg cgcttccctg ccatgctgcg cactgtgccc 2881 agcgccaccc accacatcga ggccatggtg caactgatgg ttcacttcca gtggaactgg 2941 atcgtggtgc tggtgagcga tgacgattat ggccgagaga acagccacct gctgagccag 3001 cgtctgacca acactggcga tatctgcatt gccttccagg aggttctgcc tgtaccagaa 3061 cccaaccagg ccgtgaggcc tgaggagcag gaccaactgg acaacatcct ggacaagctg 3121 cggcggacct cggcgcgtgt ggtggtgata ttctcgccag agctgagcct gcacaacttc 3181 ttccgcgagg tgctgcgctg gaacttcaca ggctttgtgt ggattgcctc tgagtcctgg 3241 gccatcgacc ctgttctaca caacctcaca gagctgcgcc acacgggcac tttcctgggc 3301 gtcaccatcc agagggtgtc catccctggc ttcagccagt tccgagtgcg ccacgacaag 3361 ccagagtatc ccatgcctaa cgagaccagc ctgaggacta cctgtaacca ggactgtgac 3421 gcctgcatga acatcaccga gtcctttaac aacgttctca tgctttcggg ggagcgtgtg 3481 gtctacagtg tgtactcggc cgtctacgcg gtagcccaca ccctccacag actcctccac 3541 tgcaaccagg tccgctgcac caagcaaatc gtctatccat ggcagctact cagggagatc 3601 tggcatgtca acttcacgct cctgggcaac cagctcttct tcgacgaaca aggggacatg 3661 ccgatgctcc tggacatcat ccagtggcaa tggggcctga gccagaaccc cttccaaagc 3721 atcgcctcct actcccccac cgagacgagg ctgacctaca ttagcaatgt gtcctggtac 3781 acccccaaca acacggtccc catatccatg tgttctaaga gttgccagcc tgggcaaatg 3841 aaaaaaccca taggcctcca cccgtgctgc ttcgagtgtg tggactgtcc gccgggcacc 3901 tacctcaacc gatcagtaga tgagtttaac tgtctgtcct gcccgggttc catgtggtct 3961 tacaagaaca acatcgcttg cttcaagcgg cggctggcct tcctggagtg gcacgaagtg 4021 cccactatcg tggtgaccat cctggccgcc ctgggcttca tcagtacgct ggccattctg 4081 ctcatcttct ggagacattt ccagacgccc atggtgcgct cggcgggcgg ccccatgtgc 4141 ttcctgatgc tggtgcccct gctgctggcg ttcgggatgg tccccgtgta tgtgggcccc 4201 cccacggtct tctcctgttt ctgccgccag gctttcttca ccgtttgctt ctccgtctgc 4261 ctctcctgca tcacggtgcg ctccttccag attgtgtgcg tcttcaagat ggccagacgc 4321 ctgccaagcg cctacggttt ctggatgcgt taccacgggc cctacgtctt tgtggccttc 4381 atcacggccg tcaaggtggc cctggtggca ggcaacatgc tggccaccac catcaacccc 4441 attggccgga ccgaccccga tgaccccaat atcataatcc tctcctgcca ccctaactac 4501 cgcaacgggc tactcttcaa caccagcatg gacttgctgc tgtccgtgct gggtttcagc 4561 ttcgcgtacg tgggcaagga actgcccacc aactacaacg aagccaagtt catcaccctc 4621 agcatgacct tctccttcac ctcctccatc tccctctgca cgttcatgtc tgtccacgat 4681 ggcgtgctgg tcaccatcat ggatctcctg gtcactgtgc tcaactttct ggccatcggc 4741 ttggggtact ttggccccaa gtgttacatg atccttttct acccggagcg caacacttca 4801 gcttatttca atagcatgat tcagggctac acgatgagga agagctaggg atccCTGCGA 4861 TATCCAGCgc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 4921 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4981 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 5041 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 5101 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 5161 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 5221 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 5281 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 5341 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 5401 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 5461 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 5521 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 5581 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 5641 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 5701 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 5761 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 5821 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 5881 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 5941 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 6001 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 6061 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 6121 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 6181 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 6241 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 6301 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 6361 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 6421 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 6481 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 6541 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 6601 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 6661 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 6721 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 6781 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 6841 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 7021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 7081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 7141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 7201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 7261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 7381 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta 7441 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 7501 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact 7561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 7621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 7681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 7741 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 7801 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 7861 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 7921 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 7981 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 8041 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 8101 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 8161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 8221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 8281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 8341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 8401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 8461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 8521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 8581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 8641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 8701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 8761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 8821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 8881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8941 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 9001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 9061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 9121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 9181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 9241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 9301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 9361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 9421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 9481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 9541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 9601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 9721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 9781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 10021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 10081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 10141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 10201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 10261 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 10321 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 10381 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 10441 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 10501 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 10561 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 10621 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 10681 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 10741 gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.