pLV2-CMV-PIAS3-3×FLAG-Puro pla

pLV2-CMV-PIAS3-3×FLAG-Puro plasmid

PVT88962

pLV2-CMV-PIAS3-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-PIAS3(human)-3×FLAG-Puro
Alias: NM_006099.3;ZMIZ5
Gene lenght: 1887bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-PIAS3-3×FLAG-Puro plasmid Description

pLV2-CMV-PIAS3-3×FLAG-Puro This is a plasmid suitable for protein expression in mammalian cells and can also be used for packaging lentivirus.

pLV2-CMV-PIAS3-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCATGGCGGA GCTGGGCGAA TTAAAGCACA TGGTGATGAG 2881 TTTCCGGGTG TCTGAGCTCC AGGTGCTTCT TGGCTTTGCT GGCCGGAACA AGAGTGGACG 2941 GAAGCACGAG CTCCTGGCCA AGGCTCTGCA CCTCCTGAAG TCCAGCTGTG CCCCTAGTGT 3001 CCAGATGAAG ATCAAAGAGC TTTACCGACG ACGCTTTCCC CGGAAGACCC TGGGGCCCTC 3061 TGATCTCTCC CTTCTCTCTT TGCCCCCTGG CACCTCTCCT GTAGGCTCCC CTGGTCCTCT 3121 AGCTCCCATT CCCCCAACGC TGTTGGCCCC TGGCACCCTG CTGGGCCCCA AGCGTGAGGT 3181 GGACATGCAC CCCCCTCTGC CCCAGCCTGT GCACCCTGAT GTCACCATGA AACCATTGCC 3241 CTTCTATGAA GTCTATGGGG AGCTCATCCG GCCCACCACC CTTGCATCCA CTTCTAGCCA 3301 GCGGTTTGAG GAAGCGCACT TTACCTTTGC CCTCACACCC CAGCAAGTGC AGCAGATTCT 3361 TACATCCAGA GAGGTTCTGC CAGGAGCCAA ATGTGATTAT ACCATACAGG TGCAGCTAAG 3421 GTTCTGTCTC TGTGAGACCA GCTGCCCCCA GGAAGATTAT TTTCCCCCCA ACCTCTTTGT 3481 CAAGGTCAAT GGGAAACTGT GCCCCCTGCC GGGTTACCTT CCCCCAACCA AGAATGGGGC 3541 CGAGCCCAAG AGGCCCAGCC GCCCCATCAA CATCACACCC CTGGCTCGAC TCTCAGCCAC 3601 TGTTCCCAAC ACCATTGTGG TCAATTGGTC ATCTGAGTTC GGACGGAATT ACTCCTTGTC 3661 TGTGTACCTG GTGAGGCAGT TGACTGCAGG AACCCTTCTA CAAAAACTCA GAGCAAAGGG 3721 TATCCGGAAC CCAGACCACT CGCGGGCACT GATCAAGGAG AAATTGACTG CTGACCCTGA 3781 CAGTGAGGTG GCCACTACAA GTCTCCGGGT GTCACTCATG TGCCCGCTAG GGAAGATGCG 3841 CCTGACTGTC CCTTGTCGTG CCCTCACCTG CGCCCACCTG CAGAGCTTCG ATGCTGCCCT 3901 TTATCTACAG ATGAATGAGA AGAAGCCTAC ATGGACATGT CCTGTGTGTG ACAAGAAGGC 3961 TCCCTATGAA TCTCTTATCA TTGATGGTTT ATTTATGGAG ATTCTTAGTT CCTGTTCAGA 4021 TTGTGATGAG ATCCAATTCA TGGAAGATGG ATCCTGGTGC CCAATGAAAC CCAAGAAGGA 4081 GGCATCTGAG GTTTGCCCCC CGCCAGGGTA TGGGCTGGAT GGCCTCCAGT ACAGCCCAGT 4141 CCAGGGGGGA GATCCATCAG AGAATAAGAA GAAGGTCGAA GTTATTGACT TGACAATAGA 4201 AAGCTCATCA GATGAGGAGG ATCTGCCCCC TACCAAGAAG CACTGTTCTG TCACCTCAGC 4261 TGCCATCCCG GCCCTACCTG GAAGCAAAGG AGTCCTGACA TCTGGCCACC AGCCATCCTC 4321 GGTGCTAAGG AGCCCTGCTA TGGGCACGTT GGGTGGGGAT TTCCTGTCCA GTCTCCCACT 4381 ACATGAGTAC CCACCTGCCT TCCCACTGGG AGCCGACATC CAAGGTTTAG ATTTATTTTC 4441 ATTTCTTCAG ACAGAGAGTC AGCACTATGG CCCCTCTGTC ATCACCTCAC TAGATGAACA 4501 GGATGCCCTT GGCCACTTCT TCCAGTACCG AGGGACCCCT TCTCACTTTC TGGGCCCACT 4561 GGCCCCCACG CTGGGGAGCT CCCACTGCAG CGCCACTCCG GCGCCCCCTC CTGGCCGTGT 4621 CAGCAGCATT GTGGCCCCTG GGGGGGCCTT GAGGGAGGGG CATGGAGGAC CCCTGCCCTC 4681 AGGTCCCTCT TTGACTGGCT GTCGGTCAGA CATCATTTCC CTGGACggat ccGACTACAA 4741 AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGta 4801 gtctagataa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc 4861 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc 4921 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc 4981 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg 5041 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg 5101 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc 5161 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc 5221 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc 5281 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg 5341 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 5401 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 5461 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5521 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5581 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5641 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5701 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5761 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5821 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5881 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg 5941 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 6001 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 6061 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 6121 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 6181 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 6241 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 6301 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 6361 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 6421 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 6481 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 6541 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca 6601 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt 6661 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 6721 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat 6781 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca 6841 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt 6901 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg 6961 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc 7021 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat 7081 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg 7141 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg 7201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 7261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 7321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 7381 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 7441 gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt 7501 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 7561 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 7621 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 7681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 7741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 7801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 7861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 7921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 7981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 8041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 8101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 8161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 8221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 8281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 8341 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 8401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 8461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 8521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 8581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 8641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 8701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 8761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 8821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 8881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 8941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 9001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 9061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 9121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 9181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 9241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 9301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 9361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 9421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 9481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 9541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 9601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 9661 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc 9721 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 9781 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa 9841 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat 9901 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa 9961 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa 10021 acttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.