pCMV-Mpeg1-3×HA-Neo plasmid

pCMV-Mpeg1-3×HA-Neo plasmid

PVT87673

pCMV-Mpeg1-3×HA-Neo plasmid Information

Plasmid name: pCMV-Mpeg1(mouse)-3×HA-Neo
Alias: NM_010821.2;mPFN2; Mpg-1; MPS1; P-2; PFN2
Gene lenght: 2142bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-3×HA-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Mpeg1-3×HA-Neo plasmid Description

pCMV-Mpeg1-3×HA-Neo This plasmid is suitable for overexpression in mammalian cells. The CMV enhancer and CMV promoter included in the plasmid can jointly initiate the efficient expression of the mouse Mpeg1 gene, promoting the C-terminal fusion expression of the Mpeg1 gene with an HA tag, which can be detected, localized, and purified using HA antibodies. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-Mpeg1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCg ccaccATGAA CAGCTTCATG 721 GCCTTGGTCC TCATCTGGAT GATAATAGCG TGTGCTGAAG CAGACAAGCC TCTTGGAGAA 781 ACGGGTACCA CTGGATTTCA AATATGCAAG AATGCCCTGA AACTACCTGT CTTGGAGGTC 841 CTACCAGGAG GAGGCTGGGA TAATCTGAGA AATGTAGACA TGGGACGGGT GATGGACTTG 901 ACATACACCA ACTGTAAGAC CACAGAAGAT GGGCAGTACA TCATCCCCGA TGAAGTGTAT 961 ACTATTCCTC AGAAAGAGAG CAACCTGGAG ATGAACTCAG AAGTCCTGGA GTCCTGGATG 1021 AATTACCAGA GTACCACCTC ACTTTCTATC AACACAGAAC TCGCCCTTTT CTCCAGAGTC 1081 AACGGCAAGT TCTCTACTGA GTTCCAAAGG ATGAAGACCC TTCAAGTAAA GGACCAAGCT 1141 GTGACTACCA GGGTTCAGGT AAGAAACCGG ATCTACACAG TGAAAACCAC CCCAACTTCA 1201 GAGCTCAGCT TGGGGTTTAC GAAGGCACTT ATGGACATCT GTGACCAACT AGAGAAAAAC 1261 CAGACGAAGA TGGCCACCTA CCTGGCAGAG CTCTTGATCC TCAACTATGG CACACACGTA 1321 ATCACTAGTG TGGATGCTGG GGCTGCACTG GTTCAGGAGG ATCACGTAAG GTCCTCCTTC 1381 CTTCTGGACA ACCAGAATAG CCAGAACACC GTGACCGCTT CTGCAGGGAT TGCCTTCTTA 1441 AACATTGTGA ACTTCAAAGT TGAAACAGAC TACATTTCTC AGACCAGTTT GACGAAGGAC 1501 TACCTGTCGA ACAGGACCAA CTCCAGGGTG CAGAGTTTTG GAGGGGTTCC CTTCTATCCA 1561 GGCATCACCT TAGAAACCTG GCAGAAGGGC ATCACTAACC ACCTAGTGGC AATAGACCGT 1621 GCTGGCTTGC CTCTGCATTT CTTCATTAAA CCTGACAAGC TACCTGGCTT GCCAGGTCCC 1681 TTGGTGAAGA AGCTGTCGAA GACAGTGGAA ACTGCTGTGA GACACTATTA CACTTTTAAC 1741 ACTCACCCAG GATGCACAAA TGTTGATTCC CCCAACTTTA ATTTTCAAGC CAATATGGAT 1801 GATGATTCCT GTGATGCGAA AGTCACCAAC TTCACCTTTG GTGGAGTTTA TCAGGAATGC 1861 ACTGAACTGT CAGGTGATGT TCTTTGCCAA AACCTGGAGC AGAAGAACCT GCTCACAGGT 1921 GATTTCTCTT GTCCCCCTGG CTACACCCCT GTCCATCTGC TCTCCCAGAC CCATGAAGAG 1981 GGTTACAGTC GTCTGGAATG TAAAAAGAAA TGCACCCTCA AGATTTTCTG CAAGACAGTG 2041 TGTGAAGATG TGTTCAGAGT GGCCAAGGCT GAATTTAGGG CTTATTGGTG TGTGGCTGCT 2101 GGCCAAGTAC CTGACAACTC AGGACTTCTC TTTGGAGGAG TCTTCACTGA CAAGACCATC 2161 AACCCTATGA CAAATGCACA GTCATGCCCA GCAGGCTACA TCCCACTGAA CCTGTTTGAA 2221 AGCCTCAAGG TATGTGTGTC CCTGGATTAT GAGTTGGGGT TCAAGTTTTC AGTCCCCTTT 2281 GGTGGGTTCT TCAGTTGTAT AATGGGGAAC CCCTTGGTTA ATTCTGATAC AGCTAAAGAC 2341 GTCAGAGCAC CATCTCTGAA AAAGTGTCCC GGGGGCTTCA GCCAACACCT AGCTGTTATC 2401 AGTGATGGAT GCCAAGTGTC CTACTGTGTC AAGGCTGGAA TCTTCACAGG AGGGTCCCTG 2461 CTCCCTGTCA GGCTCCCACC TTATACCAAA CCACCTCTTA TGAGCCAGGT TGCCACCAAC 2521 ACTGTCATAG TGACCAATAG TGAGACTGCC AGATCCTGGA TTAAGGATCC TCAGACCAAC 2581 CAGTGGAAGC TGGGAGAACC TCTGGAGCTT CGTAGGGCCA TGACAGTCAT CCATGGGGAC 2641 AGTAATGGAA TGTCAGGAGG GGAAGCTGCT GGAATCACTT TGGGAGTCAC CATAGCACTA 2701 GGAGTTGTCA TTACCTTGGC CATCTATGGT ACTCGGAAGT ACAAGAAGAA GGAATACCAG 2761 GAAATTGAGG AGCAGGAGAG TTTGGTTGGA AGCTTAGCAA CAGATGCAAC AGTCCTTAAT 2821 GGAGAAGAGG ATCCAAGTCC AGCTCTCGAG TACCCATACG ACGTACCTGA TTATGCAACT 2881 TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA CTACGCATAG 2941 TGAGGGCCCG ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 3001 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 3061 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 3121 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 3181 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 3241 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 3301 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 3361 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 3421 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 3481 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 3541 CCTCCCCGCC TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG 3601 AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG 3661 TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC 3721 ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG 3781 AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA 3841 GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 3901 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 3961 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 4021 GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 4081 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 4141 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 4201 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA 4261 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG 4321 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 4381 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 4441 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 4501 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 4561 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 4621 CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA 4681 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC 4741 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 4801 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 4861 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC 4921 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 4981 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT 5041 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT 5101 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 5161 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG 5221 GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT 5281 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG 5341 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 5401 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 5461 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA 5521 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 5581 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 5641 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 5701 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 5761 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 5821 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 5881 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 5941 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 6001 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 6061 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 6121 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 6181 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 6241 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 6301 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 6361 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 6421 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 6481 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 6541 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 6601 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 6661 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 6721 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 6781 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 6841 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 6901 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 6961 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 7021 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 7081 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 7141 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 7201 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 7261 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 7321 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 7381 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 7441 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 7501 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 7561 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 7621 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 7681 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 7741 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 7801 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 7861 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 7921 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 7981 GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT 8041 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT 8101 GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA 8161 CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.